Experiment: Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tatD and tatC are separated by 74 nucleotides tatC and tatB are separated by 2 nucleotides tatB and tatA are separated by 3 nucleotides tatA and ubiB are separated by 115 nucleotides
LU632_RS23975: tatD - 3'-5' ssDNA/RNA exonuclease TatD, at 4,571,204 to 4,571,998
tatD
LU632_RS23980: tatC - Sec-independent protein translocase subunit TatC, at 4,572,073 to 4,572,831
tatC
LU632_RS23985: tatB - Sec-independent protein translocase protein TatB, at 4,572,834 to 4,573,481
tatB
LU632_RS23990: tatA - twin-arginine translocase TatA/TatE family subunit, at 4,573,485 to 4,573,733
tatA
LU632_RS23995: ubiB - ubiquinone biosynthesis regulatory protein kinase UbiB, at 4,573,849 to 4,575,486
ubiB
Position (kb) 4572
4573
4574 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 4571.903 kb on + strand, within tatD at 4571.903 kb on + strand, within tatD at 4571.903 kb on + strand, within tatD at 4571.904 kb on - strand, within tatD at 4571.904 kb on - strand, within tatD at 4571.904 kb on - strand, within tatD at 4571.904 kb on - strand, within tatD at 4571.987 kb on + strand at 4571.988 kb on - strand at 4571.989 kb on + strand at 4572.062 kb on - strand at 4572.075 kb on - strand at 4572.075 kb on - strand at 4572.075 kb on - strand at 4572.126 kb on - strand at 4572.126 kb on - strand at 4572.279 kb on + strand, within tatC at 4572.280 kb on - strand, within tatC at 4572.283 kb on + strand, within tatC at 4572.283 kb on + strand, within tatC at 4572.284 kb on - strand, within tatC at 4572.284 kb on - strand, within tatC at 4572.381 kb on + strand, within tatC at 4572.489 kb on - strand, within tatC at 4572.534 kb on - strand, within tatC at 4572.657 kb on + strand, within tatC at 4572.658 kb on - strand, within tatC at 4572.658 kb on - strand, within tatC at 4572.700 kb on - strand, within tatC at 4572.726 kb on - strand, within tatC at 4572.726 kb on - strand, within tatC at 4572.759 kb on + strand at 4572.760 kb on - strand at 4572.768 kb on + strand at 4572.768 kb on + strand at 4572.959 kb on + strand, within tatB at 4573.148 kb on + strand, within tatB at 4573.194 kb on - strand, within tatB at 4573.195 kb on + strand, within tatB at 4573.196 kb on - strand, within tatB at 4573.196 kb on - strand, within tatB at 4573.196 kb on - strand, within tatB at 4573.248 kb on - strand, within tatB at 4573.320 kb on + strand, within tatB at 4573.423 kb on - strand at 4573.452 kb on + strand at 4573.471 kb on - strand at 4573.487 kb on - strand at 4573.661 kb on + strand, within tatA at 4573.662 kb on - strand, within tatA at 4573.722 kb on - strand at 4573.776 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS) remove 4,571,903 + tatD LU632_RS23975 0.88 -0.7 4,571,903 + tatD LU632_RS23975 0.88 +2.5 4,571,903 + tatD LU632_RS23975 0.88 -1.8 4,571,904 - tatD LU632_RS23975 0.88 +0.9 4,571,904 - tatD LU632_RS23975 0.88 +0.6 4,571,904 - tatD LU632_RS23975 0.88 +1.5 4,571,904 - tatD LU632_RS23975 0.88 -0.8 4,571,987 + +1.2 4,571,988 - -1.1 4,571,989 + -0.0 4,572,062 - -2.5 4,572,075 - -0.5 4,572,075 - -0.4 4,572,075 - -1.0 4,572,126 - +2.2 4,572,126 - -1.3 4,572,279 + tatC LU632_RS23980 0.27 +0.2 4,572,280 - tatC LU632_RS23980 0.27 -3.2 4,572,283 + tatC LU632_RS23980 0.28 -6.6 4,572,283 + tatC LU632_RS23980 0.28 -3.5 4,572,284 - tatC LU632_RS23980 0.28 -2.7 4,572,284 - tatC LU632_RS23980 0.28 -4.0 4,572,381 + tatC LU632_RS23980 0.41 -5.1 4,572,489 - tatC LU632_RS23980 0.55 -4.5 4,572,534 - tatC LU632_RS23980 0.61 -3.0 4,572,657 + tatC LU632_RS23980 0.77 -6.3 4,572,658 - tatC LU632_RS23980 0.77 -4.8 4,572,658 - tatC LU632_RS23980 0.77 -2.2 4,572,700 - tatC LU632_RS23980 0.83 -5.4 4,572,726 - tatC LU632_RS23980 0.86 -4.9 4,572,726 - tatC LU632_RS23980 0.86 -3.8 4,572,759 + -3.6 4,572,760 - -3.6 4,572,768 + -4.2 4,572,768 + +0.2 4,572,959 + tatB LU632_RS23985 0.19 -2.8 4,573,148 + tatB LU632_RS23985 0.48 -2.5 4,573,194 - tatB LU632_RS23985 0.56 -2.8 4,573,195 + tatB LU632_RS23985 0.56 -4.1 4,573,196 - tatB LU632_RS23985 0.56 -0.1 4,573,196 - tatB LU632_RS23985 0.56 -1.6 4,573,196 - tatB LU632_RS23985 0.56 +0.1 4,573,248 - tatB LU632_RS23985 0.64 -0.4 4,573,320 + tatB LU632_RS23985 0.75 -3.6 4,573,423 - -3.8 4,573,452 + -2.4 4,573,471 - -5.1 4,573,487 - -3.0 4,573,661 + tatA LU632_RS23990 0.71 -3.1 4,573,662 - tatA LU632_RS23990 0.71 +0.6 4,573,722 - +0.2 4,573,776 - -0.7
Or see this region's nucleotide sequence