Experiment: Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS12845 and cls are separated by 557 nucleotides cls and LU632_RS12855 are separated by 45 nucleotides LU632_RS12855 and oppF are separated by 227 nucleotides
LU632_RS12845: LU632_RS12845 - IS256 family transposase, at 2,444,049 to 2,445,244
_RS12845
LU632_RS12850: cls - cardiolipin synthase, at 2,445,802 to 2,447,262
cls
LU632_RS12855: LU632_RS12855 - HI1450 family dsDNA-mimic protein, at 2,447,308 to 2,447,631
_RS12855
LU632_RS12860: oppF - murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF, at 2,447,859 to 2,448,860
oppF
Position (kb)
2445
2446
2447
2448 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2445.267 kb on + strand at 2445.271 kb on + strand at 2445.303 kb on - strand at 2445.493 kb on + strand at 2445.542 kb on - strand at 2445.586 kb on - strand at 2445.626 kb on + strand at 2445.626 kb on + strand at 2445.626 kb on + strand at 2445.627 kb on - strand at 2445.658 kb on + strand at 2445.936 kb on + strand at 2446.118 kb on + strand, within cls at 2446.202 kb on + strand, within cls at 2446.498 kb on + strand, within cls at 2446.502 kb on + strand, within cls at 2446.508 kb on - strand, within cls at 2446.627 kb on + strand, within cls at 2446.689 kb on + strand, within cls at 2446.719 kb on + strand, within cls at 2447.017 kb on - strand, within cls at 2447.362 kb on - strand, within LU632_RS12855 at 2447.407 kb on - strand, within LU632_RS12855 at 2447.520 kb on - strand, within LU632_RS12855 at 2447.601 kb on + strand at 2447.602 kb on - strand at 2447.638 kb on - strand at 2447.765 kb on + strand at 2447.768 kb on + strand at 2447.768 kb on + strand at 2447.768 kb on + strand at 2447.768 kb on + strand at 2447.771 kb on + strand at 2447.771 kb on + strand at 2447.771 kb on + strand at 2448.021 kb on + strand, within oppF at 2448.021 kb on + strand, within oppF at 2448.022 kb on - strand, within oppF at 2448.083 kb on + strand, within oppF at 2448.086 kb on - strand, within oppF at 2448.107 kb on - strand, within oppF at 2448.118 kb on + strand, within oppF at 2448.118 kb on + strand, within oppF at 2448.119 kb on - strand, within oppF at 2448.148 kb on + strand, within oppF at 2448.149 kb on - strand, within oppF at 2448.149 kb on - strand, within oppF at 2448.203 kb on + strand, within oppF at 2448.203 kb on + strand, within oppF at 2448.204 kb on - strand, within oppF at 2448.207 kb on + strand, within oppF
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS) remove 2,445,267 + -0.5 2,445,271 + -1.6 2,445,303 - -0.5 2,445,493 + -0.3 2,445,542 - -2.5 2,445,586 - -0.5 2,445,626 + +1.6 2,445,626 + +4.2 2,445,626 + +2.6 2,445,627 - -0.6 2,445,658 + +0.8 2,445,936 + -2.3 2,446,118 + cls LU632_RS12850 0.22 -2.5 2,446,202 + cls LU632_RS12850 0.27 +0.7 2,446,498 + cls LU632_RS12850 0.48 -2.8 2,446,502 + cls LU632_RS12850 0.48 -1.9 2,446,508 - cls LU632_RS12850 0.48 -0.9 2,446,627 + cls LU632_RS12850 0.56 -1.3 2,446,689 + cls LU632_RS12850 0.61 -1.3 2,446,719 + cls LU632_RS12850 0.63 -0.3 2,447,017 - cls LU632_RS12850 0.83 -1.9 2,447,362 - LU632_RS12855 0.17 -0.5 2,447,407 - LU632_RS12855 0.31 -0.5 2,447,520 - LU632_RS12855 0.65 -0.8 2,447,601 + -3.0 2,447,602 - -2.3 2,447,638 - -3.9 2,447,765 + +0.1 2,447,768 + +0.8 2,447,768 + +1.7 2,447,768 + +1.6 2,447,768 + -1.9 2,447,771 + -0.3 2,447,771 + -1.5 2,447,771 + +3.2 2,448,021 + oppF LU632_RS12860 0.16 -2.3 2,448,021 + oppF LU632_RS12860 0.16 -0.7 2,448,022 - oppF LU632_RS12860 0.16 +1.5 2,448,083 + oppF LU632_RS12860 0.22 -2.1 2,448,086 - oppF LU632_RS12860 0.23 -2.3 2,448,107 - oppF LU632_RS12860 0.25 -1.7 2,448,118 + oppF LU632_RS12860 0.26 -2.7 2,448,118 + oppF LU632_RS12860 0.26 +0.8 2,448,119 - oppF LU632_RS12860 0.26 +1.5 2,448,148 + oppF LU632_RS12860 0.29 -3.2 2,448,149 - oppF LU632_RS12860 0.29 -1.1 2,448,149 - oppF LU632_RS12860 0.29 +2.7 2,448,203 + oppF LU632_RS12860 0.34 -4.1 2,448,203 + oppF LU632_RS12860 0.34 +1.8 2,448,204 - oppF LU632_RS12860 0.34 -1.7 2,448,207 + oppF LU632_RS12860 0.35 +0.4
Or see this region's nucleotide sequence