Experiment: Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 2 nucleotides thrB and thrC are separated by 3 nucleotides
LU632_RS02610: thrA - bifunctional aspartate kinase/homoserine dehydrogenase I, at 520,215 to 522,677
thrA
LU632_RS02615: thrB - homoserine kinase, at 522,680 to 523,609
thrB
LU632_RS02620: thrC - threonine synthase, at 523,613 to 524,899
thrC
Position (kb) 520
521
522
523 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 519.395 kb on - strand at 519.405 kb on - strand at 519.587 kb on - strand at 519.620 kb on + strand at 519.662 kb on - strand at 519.764 kb on - strand at 519.784 kb on + strand at 519.785 kb on - strand at 519.809 kb on - strand at 519.863 kb on - strand at 519.884 kb on - strand at 519.979 kb on + strand at 520.023 kb on - strand at 520.049 kb on - strand at 520.056 kb on - strand at 520.071 kb on + strand at 520.095 kb on + strand at 520.099 kb on - strand at 520.099 kb on - strand at 520.111 kb on - strand at 520.140 kb on - strand at 520.395 kb on - strand at 520.403 kb on + strand at 520.528 kb on + strand, within thrA at 520.580 kb on - strand, within thrA at 520.913 kb on + strand, within thrA at 520.965 kb on + strand, within thrA at 520.989 kb on + strand, within thrA at 521.021 kb on + strand, within thrA at 521.021 kb on + strand, within thrA at 521.228 kb on + strand, within thrA at 521.229 kb on - strand, within thrA at 521.281 kb on - strand, within thrA at 521.416 kb on + strand, within thrA at 521.450 kb on - strand, within thrA at 521.700 kb on - strand, within thrA at 521.700 kb on - strand, within thrA at 521.855 kb on + strand, within thrA at 521.905 kb on - strand, within thrA at 521.976 kb on + strand, within thrA at 521.976 kb on + strand, within thrA at 521.977 kb on - strand, within thrA at 521.977 kb on - strand, within thrA at 521.977 kb on - strand, within thrA at 521.989 kb on + strand, within thrA at 521.989 kb on + strand, within thrA at 521.989 kb on + strand, within thrA at 521.994 kb on + strand, within thrA at 522.061 kb on + strand, within thrA at 522.140 kb on + strand, within thrA at 522.141 kb on - strand, within thrA at 522.141 kb on - strand, within thrA at 522.166 kb on + strand, within thrA at 522.167 kb on - strand, within thrA at 522.435 kb on - strand at 522.519 kb on + strand at 522.519 kb on + strand at 522.650 kb on + strand at 522.651 kb on - strand at 522.723 kb on + strand at 522.787 kb on + strand, within thrB at 522.842 kb on - strand, within thrB at 522.869 kb on - strand, within thrB at 523.016 kb on + strand, within thrB at 523.016 kb on + strand, within thrB at 523.017 kb on - strand, within thrB at 523.017 kb on - strand, within thrB at 523.219 kb on + strand, within thrB at 523.219 kb on + strand, within thrB at 523.262 kb on + strand, within thrB at 523.263 kb on - strand, within thrB at 523.263 kb on - strand, within thrB at 523.384 kb on + strand, within thrB at 523.427 kb on - strand, within thrB at 523.432 kb on + strand, within thrB at 523.499 kb on - strand, within thrB at 523.539 kb on - strand at 523.539 kb on - strand at 523.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS) remove 519,395 - +1.8 519,405 - +0.9 519,587 - +1.5 519,620 + +1.4 519,662 - +0.5 519,764 - -0.7 519,784 + +1.3 519,785 - +0.4 519,809 - -2.7 519,863 - -2.6 519,884 - -0.2 519,979 + -1.0 520,023 - -0.5 520,049 - -2.1 520,056 - -2.7 520,071 + -1.0 520,095 + +0.9 520,099 - +0.2 520,099 - -3.4 520,111 - -1.7 520,140 - -2.7 520,395 - +2.9 520,403 + -1.9 520,528 + thrA LU632_RS02610 0.13 -3.5 520,580 - thrA LU632_RS02610 0.15 +0.1 520,913 + thrA LU632_RS02610 0.28 -3.3 520,965 + thrA LU632_RS02610 0.30 -1.7 520,989 + thrA LU632_RS02610 0.31 -2.3 521,021 + thrA LU632_RS02610 0.33 -1.2 521,021 + thrA LU632_RS02610 0.33 -0.9 521,228 + thrA LU632_RS02610 0.41 -1.7 521,229 - thrA LU632_RS02610 0.41 -1.5 521,281 - thrA LU632_RS02610 0.43 -0.5 521,416 + thrA LU632_RS02610 0.49 -0.9 521,450 - thrA LU632_RS02610 0.50 +0.1 521,700 - thrA LU632_RS02610 0.60 -2.5 521,700 - thrA LU632_RS02610 0.60 -1.7 521,855 + thrA LU632_RS02610 0.67 -1.2 521,905 - thrA LU632_RS02610 0.69 +0.5 521,976 + thrA LU632_RS02610 0.71 -0.5 521,976 + thrA LU632_RS02610 0.71 -2.2 521,977 - thrA LU632_RS02610 0.72 -1.7 521,977 - thrA LU632_RS02610 0.72 -2.1 521,977 - thrA LU632_RS02610 0.72 +1.1 521,989 + thrA LU632_RS02610 0.72 -2.7 521,989 + thrA LU632_RS02610 0.72 +1.1 521,989 + thrA LU632_RS02610 0.72 -1.2 521,994 + thrA LU632_RS02610 0.72 -0.8 522,061 + thrA LU632_RS02610 0.75 -2.4 522,140 + thrA LU632_RS02610 0.78 -0.5 522,141 - thrA LU632_RS02610 0.78 -1.6 522,141 - thrA LU632_RS02610 0.78 -0.9 522,166 + thrA LU632_RS02610 0.79 -2.5 522,167 - thrA LU632_RS02610 0.79 -1.3 522,435 - -2.3 522,519 + -1.8 522,519 + +2.1 522,650 + -1.2 522,651 - +1.7 522,723 + +0.7 522,787 + thrB LU632_RS02615 0.12 +0.1 522,842 - thrB LU632_RS02615 0.17 -1.0 522,869 - thrB LU632_RS02615 0.20 -0.5 523,016 + thrB LU632_RS02615 0.36 -0.9 523,016 + thrB LU632_RS02615 0.36 +1.1 523,017 - thrB LU632_RS02615 0.36 -2.3 523,017 - thrB LU632_RS02615 0.36 +0.1 523,219 + thrB LU632_RS02615 0.58 +1.1 523,219 + thrB LU632_RS02615 0.58 -3.9 523,262 + thrB LU632_RS02615 0.63 -2.6 523,263 - thrB LU632_RS02615 0.63 -0.9 523,263 - thrB LU632_RS02615 0.63 -1.9 523,384 + thrB LU632_RS02615 0.76 -0.9 523,427 - thrB LU632_RS02615 0.80 +1.1 523,432 + thrB LU632_RS02615 0.81 -2.1 523,499 - thrB LU632_RS02615 0.88 -2.5 523,539 - -2.1 523,539 - -1.5 523,568 - -3.2
Or see this region's nucleotide sequence