Experiment: Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mpl and LU632_RS02140 are separated by 61 nucleotides LU632_RS02140 and pmbA are separated by 166 nucleotides
LU632_RS02135: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 423,703 to 425,082
mpl
LU632_RS02140: LU632_RS02140 - ribosome-associated protein, at 425,144 to 425,683
_RS02140
LU632_RS02145: pmbA - metalloprotease PmbA, at 425,850 to 427,190
pmbA
Position (kb) 425
426 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 424.186 kb on + strand, within mpl at 424.187 kb on - strand, within mpl at 424.300 kb on - strand, within mpl at 424.322 kb on + strand, within mpl at 424.322 kb on + strand, within mpl at 424.322 kb on + strand, within mpl at 424.323 kb on - strand, within mpl at 424.323 kb on - strand, within mpl at 424.618 kb on - strand, within mpl at 424.674 kb on - strand, within mpl at 424.682 kb on - strand, within mpl at 424.725 kb on + strand, within mpl at 424.791 kb on + strand, within mpl at 425.109 kb on - strand at 425.283 kb on - strand, within LU632_RS02140 at 425.365 kb on + strand, within LU632_RS02140 at 425.445 kb on - strand, within LU632_RS02140 at 425.550 kb on - strand, within LU632_RS02140 at 425.730 kb on + strand at 425.757 kb on + strand at 425.775 kb on + strand at 425.775 kb on - strand at 425.833 kb on - strand at 425.906 kb on - strand at 426.141 kb on + strand, within pmbA at 426.308 kb on + strand, within pmbA at 426.344 kb on - strand, within pmbA at 426.344 kb on - strand, within pmbA at 426.382 kb on - strand, within pmbA at 426.450 kb on + strand, within pmbA at 426.450 kb on + strand, within pmbA at 426.450 kb on + strand, within pmbA at 426.451 kb on - strand, within pmbA at 426.456 kb on + strand, within pmbA at 426.456 kb on + strand, within pmbA at 426.456 kb on + strand, within pmbA at 426.456 kb on + strand, within pmbA at 426.457 kb on - strand, within pmbA at 426.457 kb on - strand, within pmbA at 426.457 kb on - strand, within pmbA at 426.458 kb on + strand, within pmbA at 426.458 kb on + strand, within pmbA at 426.461 kb on + strand, within pmbA at 426.527 kb on - strand, within pmbA at 426.548 kb on + strand, within pmbA at 426.548 kb on + strand, within pmbA at 426.548 kb on + strand, within pmbA at 426.630 kb on - strand, within pmbA
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS) remove 424,186 + mpl LU632_RS02135 0.35 +0.4 424,187 - mpl LU632_RS02135 0.35 -2.4 424,300 - mpl LU632_RS02135 0.43 +1.1 424,322 + mpl LU632_RS02135 0.45 -1.2 424,322 + mpl LU632_RS02135 0.45 -1.7 424,322 + mpl LU632_RS02135 0.45 +1.1 424,323 - mpl LU632_RS02135 0.45 +0.5 424,323 - mpl LU632_RS02135 0.45 +1.1 424,618 - mpl LU632_RS02135 0.66 -1.9 424,674 - mpl LU632_RS02135 0.70 -0.5 424,682 - mpl LU632_RS02135 0.71 +0.5 424,725 + mpl LU632_RS02135 0.74 -2.4 424,791 + mpl LU632_RS02135 0.79 -1.9 425,109 - -2.2 425,283 - LU632_RS02140 0.26 -4.8 425,365 + LU632_RS02140 0.41 -1.7 425,445 - LU632_RS02140 0.56 +1.1 425,550 - LU632_RS02140 0.75 -2.4 425,730 + -1.4 425,757 + +0.7 425,775 + -3.8 425,775 - -1.5 425,833 - -0.7 425,906 - -2.8 426,141 + pmbA LU632_RS02145 0.22 -0.1 426,308 + pmbA LU632_RS02145 0.34 -2.4 426,344 - pmbA LU632_RS02145 0.37 -0.2 426,344 - pmbA LU632_RS02145 0.37 -4.2 426,382 - pmbA LU632_RS02145 0.40 -0.5 426,450 + pmbA LU632_RS02145 0.45 -0.8 426,450 + pmbA LU632_RS02145 0.45 -2.7 426,450 + pmbA LU632_RS02145 0.45 -0.6 426,451 - pmbA LU632_RS02145 0.45 +1.6 426,456 + pmbA LU632_RS02145 0.45 +2.4 426,456 + pmbA LU632_RS02145 0.45 -2.1 426,456 + pmbA LU632_RS02145 0.45 -1.5 426,456 + pmbA LU632_RS02145 0.45 -0.9 426,457 - pmbA LU632_RS02145 0.45 -1.5 426,457 - pmbA LU632_RS02145 0.45 -3.2 426,457 - pmbA LU632_RS02145 0.45 +0.2 426,458 + pmbA LU632_RS02145 0.45 -1.6 426,458 + pmbA LU632_RS02145 0.45 -1.2 426,461 + pmbA LU632_RS02145 0.46 -3.1 426,527 - pmbA LU632_RS02145 0.50 -1.7 426,548 + pmbA LU632_RS02145 0.52 -3.0 426,548 + pmbA LU632_RS02145 0.52 -3.6 426,548 + pmbA LU632_RS02145 0.52 +0.2 426,630 - pmbA LU632_RS02145 0.58 +1.7
Or see this region's nucleotide sequence