Experiment: Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frsA and crl are separated by 104 nucleotides crl and LU632_RS01175 are separated by 79 nucleotides LU632_RS01175 and LU632_RS01180 are separated by 48 nucleotides LU632_RS01180 and proB are separated by 321 nucleotides
LU632_RS01165: frsA - esterase FrsA, at 247,716 to 248,948
frsA
LU632_RS01170: crl - sigma factor-binding protein Crl, at 249,053 to 249,454
crl
LU632_RS01175: LU632_RS01175 - porin, at 249,534 to 249,732
_RS01175
LU632_RS01180: LU632_RS01180 - hypothetical protein, at 249,781 to 249,870
_RS01180
LU632_RS01185: proB - glutamate 5-kinase, at 250,192 to 251,295
proB
Position (kb) 249
250 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 248.788 kb on - strand, within frsA at 248.788 kb on - strand, within frsA at 248.887 kb on + strand at 248.942 kb on - strand at 249.033 kb on + strand at 249.089 kb on - strand at 249.183 kb on + strand, within crl at 249.257 kb on + strand, within crl at 249.258 kb on - strand, within crl at 249.263 kb on + strand, within crl at 249.263 kb on + strand, within crl at 249.264 kb on - strand, within crl at 249.267 kb on - strand, within crl at 249.279 kb on - strand, within crl at 249.355 kb on + strand, within crl at 249.355 kb on + strand, within crl at 249.356 kb on - strand, within crl at 249.356 kb on - strand, within crl at 249.401 kb on - strand, within crl at 249.509 kb on + strand at 249.510 kb on - strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.519 kb on + strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.520 kb on - strand at 249.528 kb on - strand at 249.652 kb on + strand, within LU632_RS01175 at 249.653 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.656 kb on - strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.676 kb on + strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.677 kb on - strand, within LU632_RS01175 at 249.687 kb on - strand, within LU632_RS01175 at 249.731 kb on + strand at 249.785 kb on + strand at 249.785 kb on + strand at 249.810 kb on + strand, within LU632_RS01180 at 250.021 kb on - strand at 250.149 kb on + strand at 250.149 kb on + strand at 250.163 kb on + strand at 250.164 kb on - strand at 250.170 kb on - strand at 250.174 kb on - strand at 250.238 kb on + strand at 250.272 kb on + strand at 250.273 kb on - strand at 250.273 kb on - strand at 250.273 kb on - strand at 250.416 kb on - strand, within proB at 250.472 kb on - strand, within proB at 250.482 kb on + strand, within proB at 250.482 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.484 kb on + strand, within proB at 250.485 kb on - strand, within proB at 250.485 kb on - strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.553 kb on + strand, within proB at 250.554 kb on - strand, within proB at 250.575 kb on + strand, within proB at 250.575 kb on + strand, within proB at 250.576 kb on - strand, within proB at 250.576 kb on - strand, within proB at 250.809 kb on - strand, within proB
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS) remove 248,788 - frsA LU632_RS01165 0.87 -1.4 248,788 - frsA LU632_RS01165 0.87 +0.7 248,887 + +1.2 248,942 - +0.0 249,033 + +1.4 249,089 - -1.8 249,183 + crl LU632_RS01170 0.32 -1.2 249,257 + crl LU632_RS01170 0.51 +1.0 249,258 - crl LU632_RS01170 0.51 +2.8 249,263 + crl LU632_RS01170 0.52 +0.9 249,263 + crl LU632_RS01170 0.52 +0.7 249,264 - crl LU632_RS01170 0.52 +3.3 249,267 - crl LU632_RS01170 0.53 +1.6 249,279 - crl LU632_RS01170 0.56 +1.8 249,355 + crl LU632_RS01170 0.75 +3.6 249,355 + crl LU632_RS01170 0.75 -0.8 249,356 - crl LU632_RS01170 0.75 -4.0 249,356 - crl LU632_RS01170 0.75 +1.1 249,401 - crl LU632_RS01170 0.87 +0.8 249,509 + +1.2 249,510 - +0.3 249,519 + +1.0 249,519 + +0.1 249,519 + -0.8 249,519 + -1.4 249,520 - -1.4 249,520 - -1.2 249,520 - +2.2 249,520 - -0.5 249,528 - -0.3 249,652 + LU632_RS01175 0.59 +0.2 249,653 - LU632_RS01175 0.60 -1.2 249,656 - LU632_RS01175 0.61 -0.4 249,656 - LU632_RS01175 0.61 -2.7 249,656 - LU632_RS01175 0.61 +1.1 249,676 + LU632_RS01175 0.71 -1.3 249,676 + LU632_RS01175 0.71 +0.4 249,676 + LU632_RS01175 0.71 +1.2 249,677 - LU632_RS01175 0.72 -0.5 249,677 - LU632_RS01175 0.72 -1.0 249,677 - LU632_RS01175 0.72 +0.2 249,687 - LU632_RS01175 0.77 +0.5 249,731 + -2.0 249,785 + -1.2 249,785 + +1.2 249,810 + LU632_RS01180 0.32 -1.7 250,021 - -0.7 250,149 + -0.5 250,149 + -0.5 250,163 + -1.0 250,164 - +2.5 250,170 - -0.3 250,174 - -1.1 250,238 + -2.2 250,272 + +1.4 250,273 - +0.4 250,273 - -1.7 250,273 - +0.0 250,416 - proB LU632_RS01185 0.20 +0.3 250,472 - proB LU632_RS01185 0.25 -1.2 250,482 + proB LU632_RS01185 0.26 -1.6 250,482 + proB LU632_RS01185 0.26 -1.8 250,484 + proB LU632_RS01185 0.26 -0.1 250,484 + proB LU632_RS01185 0.26 +2.9 250,485 - proB LU632_RS01185 0.27 -1.3 250,485 - proB LU632_RS01185 0.27 -2.0 250,553 + proB LU632_RS01185 0.33 +0.5 250,553 + proB LU632_RS01185 0.33 -0.4 250,553 + proB LU632_RS01185 0.33 +0.5 250,554 - proB LU632_RS01185 0.33 -2.4 250,575 + proB LU632_RS01185 0.35 -2.2 250,575 + proB LU632_RS01185 0.35 +1.2 250,576 - proB LU632_RS01185 0.35 +0.2 250,576 - proB LU632_RS01185 0.35 -3.5 250,809 - proB LU632_RS01185 0.56 -0.5
Or see this region's nucleotide sequence