Experiment: Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purD and purH are separated by 14 nucleotides purH and LU632_RS01025 are separated by 590 nucleotides
LU632_RS01015: purD - phosphoribosylamine--glycine ligase, at 217,496 to 218,776
purD
LU632_RS01020: purH - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 218,791 to 220,380
purH
LU632_RS01025: LU632_RS01025 - 16S ribosomal RNA, at 220,971 to 222,512
_RS01025
Position (kb) 218
219
220
221 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 217.833 kb on - strand, within purD at 217.915 kb on + strand, within purD at 218.131 kb on + strand, within purD at 218.132 kb on - strand, within purD at 218.132 kb on - strand, within purD at 218.227 kb on + strand, within purD at 218.227 kb on + strand, within purD at 218.320 kb on + strand, within purD at 218.321 kb on - strand, within purD at 218.366 kb on - strand, within purD at 218.366 kb on - strand, within purD at 218.372 kb on + strand, within purD at 218.373 kb on - strand, within purD at 218.377 kb on - strand, within purD at 218.377 kb on - strand, within purD at 218.621 kb on - strand, within purD at 218.766 kb on - strand at 218.793 kb on - strand at 219.030 kb on + strand, within purH at 219.067 kb on - strand, within purH at 219.100 kb on - strand, within purH at 219.112 kb on + strand, within purH at 219.113 kb on - strand, within purH at 219.193 kb on + strand, within purH at 219.194 kb on - strand, within purH at 219.316 kb on - strand, within purH at 219.334 kb on - strand, within purH at 219.423 kb on + strand, within purH at 219.471 kb on - strand, within purH at 219.597 kb on - strand, within purH at 219.661 kb on + strand, within purH at 219.662 kb on - strand, within purH at 219.662 kb on - strand, within purH at 219.662 kb on - strand, within purH at 219.665 kb on + strand, within purH at 219.665 kb on + strand, within purH at 219.666 kb on - strand, within purH at 219.780 kb on - strand, within purH at 219.803 kb on + strand, within purH at 219.834 kb on + strand, within purH at 219.857 kb on - strand, within purH at 219.891 kb on + strand, within purH at 220.105 kb on + strand, within purH at 220.106 kb on - strand, within purH at 220.388 kb on - strand at 220.409 kb on + strand at 220.410 kb on - strand at 220.414 kb on - strand at 220.422 kb on + strand at 220.423 kb on - strand at 220.650 kb on - strand at 220.650 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS) remove 217,833 - purD LU632_RS01015 0.26 -2.1 217,915 + purD LU632_RS01015 0.33 -2.9 218,131 + purD LU632_RS01015 0.50 +2.0 218,132 - purD LU632_RS01015 0.50 -0.4 218,132 - purD LU632_RS01015 0.50 -3.8 218,227 + purD LU632_RS01015 0.57 -1.8 218,227 + purD LU632_RS01015 0.57 -3.1 218,320 + purD LU632_RS01015 0.64 -1.6 218,321 - purD LU632_RS01015 0.64 +1.2 218,366 - purD LU632_RS01015 0.68 -1.6 218,366 - purD LU632_RS01015 0.68 +0.2 218,372 + purD LU632_RS01015 0.68 -1.6 218,373 - purD LU632_RS01015 0.68 -3.8 218,377 - purD LU632_RS01015 0.69 -2.0 218,377 - purD LU632_RS01015 0.69 -3.1 218,621 - purD LU632_RS01015 0.88 -0.8 218,766 - +0.2 218,793 - -0.9 219,030 + purH LU632_RS01020 0.15 -3.2 219,067 - purH LU632_RS01020 0.17 +1.2 219,100 - purH LU632_RS01020 0.19 -0.4 219,112 + purH LU632_RS01020 0.20 +0.2 219,113 - purH LU632_RS01020 0.20 -1.8 219,193 + purH LU632_RS01020 0.25 -1.8 219,194 - purH LU632_RS01020 0.25 -0.8 219,316 - purH LU632_RS01020 0.33 +1.2 219,334 - purH LU632_RS01020 0.34 -2.7 219,423 + purH LU632_RS01020 0.40 -3.0 219,471 - purH LU632_RS01020 0.43 -0.8 219,597 - purH LU632_RS01020 0.51 -1.4 219,661 + purH LU632_RS01020 0.55 -1.4 219,662 - purH LU632_RS01020 0.55 -1.6 219,662 - purH LU632_RS01020 0.55 -0.4 219,662 - purH LU632_RS01020 0.55 -1.6 219,665 + purH LU632_RS01020 0.55 +1.2 219,665 + purH LU632_RS01020 0.55 -0.4 219,666 - purH LU632_RS01020 0.55 -0.8 219,780 - purH LU632_RS01020 0.62 +1.2 219,803 + purH LU632_RS01020 0.64 -2.8 219,834 + purH LU632_RS01020 0.66 -1.6 219,857 - purH LU632_RS01020 0.67 -0.4 219,891 + purH LU632_RS01020 0.69 -3.3 220,105 + purH LU632_RS01020 0.83 -2.5 220,106 - purH LU632_RS01020 0.83 +0.2 220,388 - -0.8 220,409 + -2.0 220,410 - +0.9 220,414 - +1.6 220,422 + -4.1 220,423 - +0.6 220,650 - -1.8 220,650 - -2.6
Or see this region's nucleotide sequence