Experiment: Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LU632_RS00950 and thiS are separated by 2 nucleotides thiS and LU632_RS00960 overlap by 17 nucleotides LU632_RS00960 and thiE overlap by 11 nucleotides thiE and thiC overlap by 4 nucleotides
LU632_RS00950: LU632_RS00950 - thiazole synthase, at 207,517 to 208,293
_RS00950
LU632_RS00955: thiS - sulfur carrier protein ThiS, at 208,296 to 208,496
thiS
LU632_RS00960: LU632_RS00960 - HesA/MoeB/ThiF family protein, at 208,480 to 209,241
_RS00960
LU632_RS00965: thiE - thiamine phosphate synthase, at 209,231 to 209,878
thiE
LU632_RS00970: thiC - phosphomethylpyrimidine synthase ThiC, at 209,875 to 211,809
thiC
Position (kb) 209
210 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 208.289 kb on - strand at 208.298 kb on - strand at 208.482 kb on - strand at 208.591 kb on - strand, within LU632_RS00960 at 208.657 kb on + strand, within LU632_RS00960 at 208.658 kb on - strand, within LU632_RS00960 at 208.892 kb on - strand, within LU632_RS00960 at 209.103 kb on - strand, within LU632_RS00960 at 209.176 kb on - strand at 209.212 kb on - strand at 209.432 kb on + strand, within thiE at 209.433 kb on - strand, within thiE at 209.433 kb on - strand, within thiE at 209.488 kb on + strand, within thiE at 209.489 kb on - strand, within thiE at 209.503 kb on + strand, within thiE at 209.504 kb on - strand, within thiE at 209.505 kb on + strand, within thiE at 209.505 kb on + strand, within thiE at 209.505 kb on + strand, within thiE at 209.506 kb on - strand, within thiE at 209.506 kb on - strand, within thiE at 209.609 kb on + strand, within thiE at 209.609 kb on + strand, within thiE at 209.630 kb on + strand, within thiE at 209.630 kb on + strand, within thiE at 209.631 kb on - strand, within thiE at 209.668 kb on - strand, within thiE at 209.668 kb on - strand, within thiE at 209.878 kb on + strand at 209.915 kb on + strand at 209.916 kb on - strand at 210.320 kb on + strand, within thiC at 210.320 kb on + strand, within thiC at 210.320 kb on + strand, within thiC at 210.321 kb on - strand, within thiC at 210.490 kb on + strand, within thiC at 210.491 kb on - strand, within thiC at 210.640 kb on + strand, within thiC at 210.648 kb on - strand, within thiC at 210.782 kb on - strand, within thiC at 210.786 kb on - strand, within thiC at 210.819 kb on - strand, within thiC at 210.819 kb on - strand, within thiC
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 4 Main Stem (TE2-S-C4-MS) remove 208,289 - +3.0 208,298 - -1.4 208,482 - -0.0 208,591 - LU632_RS00960 0.15 +0.7 208,657 + LU632_RS00960 0.23 +0.7 208,658 - LU632_RS00960 0.23 -0.4 208,892 - LU632_RS00960 0.54 +1.4 209,103 - LU632_RS00960 0.82 +0.9 209,176 - -0.2 209,212 - -0.8 209,432 + thiE LU632_RS00965 0.31 -3.8 209,433 - thiE LU632_RS00965 0.31 -0.8 209,433 - thiE LU632_RS00965 0.31 -2.4 209,488 + thiE LU632_RS00965 0.40 -0.4 209,489 - thiE LU632_RS00965 0.40 +1.2 209,503 + thiE LU632_RS00965 0.42 +1.0 209,504 - thiE LU632_RS00965 0.42 +0.9 209,505 + thiE LU632_RS00965 0.42 -0.2 209,505 + thiE LU632_RS00965 0.42 -1.2 209,505 + thiE LU632_RS00965 0.42 +1.5 209,506 - thiE LU632_RS00965 0.42 -2.5 209,506 - thiE LU632_RS00965 0.42 +1.1 209,609 + thiE LU632_RS00965 0.58 +0.6 209,609 + thiE LU632_RS00965 0.58 -0.1 209,630 + thiE LU632_RS00965 0.62 +0.2 209,630 + thiE LU632_RS00965 0.62 -0.3 209,631 - thiE LU632_RS00965 0.62 -1.1 209,668 - thiE LU632_RS00965 0.67 -0.4 209,668 - thiE LU632_RS00965 0.67 -2.6 209,878 + +2.2 209,915 + -0.3 209,916 - +0.7 210,320 + thiC LU632_RS00970 0.23 +1.2 210,320 + thiC LU632_RS00970 0.23 +2.6 210,320 + thiC LU632_RS00970 0.23 -0.8 210,321 - thiC LU632_RS00970 0.23 -1.4 210,490 + thiC LU632_RS00970 0.32 +1.9 210,491 - thiC LU632_RS00970 0.32 -0.5 210,640 + thiC LU632_RS00970 0.40 +0.4 210,648 - thiC LU632_RS00970 0.40 +0.3 210,782 - thiC LU632_RS00970 0.47 -1.4 210,786 - thiC LU632_RS00970 0.47 -2.5 210,819 - thiC LU632_RS00970 0.49 -0.3 210,819 - thiC LU632_RS00970 0.49 +0.2
Or see this region's nucleotide sequence