Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_02532

Experiment: K5

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	K5
2,643,013	-				+0.8
2,643,127	+				+1.5
2,643,354	+				-0.1
2,643,439	+				+0.0
2,643,529	+				+0.7
2,643,789	-				+0.4
2,643,853	+				+0.4
2,645,270	+				-2.0
2,645,270	+				-0.4
2,645,270	+				+0.7
2,645,270	+				-0.2
2,645,271	-				+0.4
2,645,271	-				+0.4
2,645,271	-				-0.8
2,645,272	+				+0.5
2,645,273	-				+0.4
2,645,361	-		OHPLBJKB_02533	0.13	+0.7
2,645,416	-		OHPLBJKB_02533	0.22	+0.4
2,645,491	-		OHPLBJKB_02533	0.33	-0.2
2,645,601	+		OHPLBJKB_02533	0.50	-0.4
2,645,602	-		OHPLBJKB_02533	0.50	+0.2
2,645,602	-		OHPLBJKB_02533	0.50	+0.3
2,645,665	+		OHPLBJKB_02533	0.60	-1.2
2,645,665	+		OHPLBJKB_02533	0.60	+0.9
2,645,788	+		OHPLBJKB_02533	0.79	+0.3
2,645,788	+		OHPLBJKB_02533	0.79	+1.5
2,645,788	+		OHPLBJKB_02533	0.79	-1.2
2,645,788	+		OHPLBJKB_02533	0.79	-0.9
2,645,788	+		OHPLBJKB_02533	0.79	-0.5
2,645,789	-		OHPLBJKB_02533	0.79	+0.1
2,645,789	-		OHPLBJKB_02533	0.79	+0.5
2,645,789	-		OHPLBJKB_02533	0.79	+1.1
2,645,789	-		OHPLBJKB_02533	0.79	-0.6
2,645,790	+		OHPLBJKB_02533	0.79	+0.4
2,645,790	+		OHPLBJKB_02533	0.79	-1.1
2,645,790	+		OHPLBJKB_02533	0.79	+0.9
2,645,790	+		OHPLBJKB_02533	0.79	-0.0
2,645,791	-		OHPLBJKB_02533	0.79	-0.1
2,645,801	+		OHPLBJKB_02533	0.81	+0.8
2,645,801	+		OHPLBJKB_02533	0.81	-2.1
2,645,802	-		OHPLBJKB_02533	0.81	+0.3
2,645,877	+				-1.0
2,645,942	+				-1.1
2,645,943	-				-1.4