Experiment: K5
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OHPLBJKB_01179 and OHPLBJKB_01180 are separated by 27 nucleotides OHPLBJKB_01180 and OHPLBJKB_01181 are separated by 16 nucleotides OHPLBJKB_01181 and OHPLBJKB_01182 are separated by 95 nucleotides OHPLBJKB_01182 and OHPLBJKB_01183 are separated by 16 nucleotides
OHPLBJKB_01179: OHPLBJKB_01179 - Cysteine desulfurase IscS, at 1,211,576 to 1,212,790
_01179
OHPLBJKB_01180: OHPLBJKB_01180 - Iron-sulfur cluster assembly scaffold protein IscU, at 1,212,818 to 1,213,204
_01180
OHPLBJKB_01181: OHPLBJKB_01181 - Iron-binding protein IscA, at 1,213,221 to 1,213,544
_01181
OHPLBJKB_01182: OHPLBJKB_01182 - Co-chaperone protein HscB, at 1,213,640 to 1,214,155
_01182
OHPLBJKB_01183: OHPLBJKB_01183 - Chaperone protein HscA, at 1,214,172 to 1,216,022
_01183
Position (kb)
1212
1213
1214 Strain fitness (log2 ratio)
-1
0
1 at 1212.743 kb on + strand at 1213.180 kb on + strand at 1213.180 kb on + strand at 1213.202 kb on + strand at 1213.202 kb on + strand at 1213.258 kb on + strand, within OHPLBJKB_01181 at 1213.338 kb on + strand, within OHPLBJKB_01181 at 1213.484 kb on + strand, within OHPLBJKB_01181 at 1213.847 kb on + strand, within OHPLBJKB_01182 at 1214.104 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K5 remove 1,212,743 + -1.0 1,213,180 + +0.4 1,213,180 + +1.7 1,213,202 + -0.1 1,213,202 + +0.0 1,213,258 + OHPLBJKB_01181 0.11 -0.3 1,213,338 + OHPLBJKB_01181 0.36 -1.0 1,213,484 + OHPLBJKB_01181 0.81 +1.0 1,213,847 + OHPLBJKB_01182 0.40 -0.6 1,214,104 + +0.4
Or see this region's nucleotide sequence