Experiment: Rhamnogalacturonan I from potato pectic fiber 2.5 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS14020 and HMPREF1181_RS14025 are separated by 37 nucleotides HMPREF1181_RS14025 and HMPREF1181_RS14030 are separated by 97 nucleotides HMPREF1181_RS14030 and HMPREF1181_RS14035 are separated by 150 nucleotides
HMPREF1181_RS14020: HMPREF1181_RS14020 - electron transfer flavoprotein subunit alpha/FixB family protein, at 324,906 to 325,925
_RS14020
HMPREF1181_RS14025: HMPREF1181_RS14025 - acyl-CoA dehydrogenase family protein, at 325,963 to 327,669
_RS14025
HMPREF1181_RS14030: HMPREF1181_RS14030 - N-acetyltransferase, at 327,767 to 328,285
_RS14030
HMPREF1181_RS14035: HMPREF1181_RS14035 - chaperone modulator CbpM, at 328,436 to 328,732
_RS14035
Position (kb)
325
326
327
328 Strain fitness (log2 ratio)
-2
-1
0
1 at 325.063 kb on + strand, within HMPREF1181_RS14020 at 325.094 kb on - strand, within HMPREF1181_RS14020 at 325.229 kb on - strand, within HMPREF1181_RS14020 at 325.503 kb on + strand, within HMPREF1181_RS14020 at 325.507 kb on + strand, within HMPREF1181_RS14020 at 325.741 kb on - strand, within HMPREF1181_RS14020 at 325.899 kb on - strand at 325.905 kb on + strand at 325.919 kb on + strand at 325.962 kb on + strand at 326.092 kb on + strand at 326.675 kb on - strand, within HMPREF1181_RS14025 at 326.697 kb on - strand, within HMPREF1181_RS14025 at 326.816 kb on + strand, within HMPREF1181_RS14025 at 326.816 kb on + strand, within HMPREF1181_RS14025 at 326.865 kb on - strand, within HMPREF1181_RS14025 at 327.030 kb on - strand, within HMPREF1181_RS14025 at 327.178 kb on + strand, within HMPREF1181_RS14025 at 327.585 kb on - strand at 327.590 kb on - strand at 327.631 kb on - strand at 327.672 kb on + strand at 327.731 kb on + strand at 327.744 kb on - strand at 327.768 kb on + strand at 327.768 kb on + strand at 327.768 kb on + strand at 327.773 kb on - strand at 327.831 kb on + strand, within HMPREF1181_RS14030 at 327.859 kb on + strand, within HMPREF1181_RS14030 at 327.864 kb on + strand, within HMPREF1181_RS14030 at 327.880 kb on + strand, within HMPREF1181_RS14030 at 327.880 kb on + strand, within HMPREF1181_RS14030 at 327.880 kb on + strand, within HMPREF1181_RS14030 at 327.881 kb on - strand, within HMPREF1181_RS14030 at 327.900 kb on + strand, within HMPREF1181_RS14030 at 327.931 kb on + strand, within HMPREF1181_RS14030 at 327.931 kb on + strand, within HMPREF1181_RS14030 at 327.931 kb on + strand, within HMPREF1181_RS14030 at 327.994 kb on + strand, within HMPREF1181_RS14030 at 327.994 kb on + strand, within HMPREF1181_RS14030 at 328.030 kb on + strand, within HMPREF1181_RS14030 at 328.030 kb on + strand, within HMPREF1181_RS14030 at 328.030 kb on + strand, within HMPREF1181_RS14030 at 328.031 kb on - strand, within HMPREF1181_RS14030 at 328.036 kb on + strand, within HMPREF1181_RS14030 at 328.036 kb on + strand, within HMPREF1181_RS14030 at 328.037 kb on - strand, within HMPREF1181_RS14030 at 328.037 kb on - strand, within HMPREF1181_RS14030 at 328.041 kb on + strand, within HMPREF1181_RS14030 at 328.068 kb on + strand, within HMPREF1181_RS14030 at 328.069 kb on - strand, within HMPREF1181_RS14030 at 328.121 kb on + strand, within HMPREF1181_RS14030 at 328.122 kb on - strand, within HMPREF1181_RS14030 at 328.163 kb on + strand, within HMPREF1181_RS14030 at 328.170 kb on + strand, within HMPREF1181_RS14030 at 328.171 kb on - strand, within HMPREF1181_RS14030 at 328.230 kb on + strand, within HMPREF1181_RS14030 at 328.242 kb on + strand at 328.269 kb on - strand at 328.310 kb on - strand at 328.364 kb on - strand at 328.400 kb on - strand at 328.426 kb on - strand at 328.437 kb on + strand at 328.468 kb on + strand, within HMPREF1181_RS14035 at 328.469 kb on - strand, within HMPREF1181_RS14035 at 328.496 kb on - strand, within HMPREF1181_RS14035 at 328.563 kb on + strand, within HMPREF1181_RS14035 at 328.563 kb on + strand, within HMPREF1181_RS14035 at 328.568 kb on - strand, within HMPREF1181_RS14035 at 328.641 kb on - strand, within HMPREF1181_RS14035 at 328.641 kb on - strand, within HMPREF1181_RS14035
Per-strain Table
Position Strand Gene LocusTag Fraction Rhamnogalacturonan I from potato pectic fiber 2.5 mg/mL (C) remove 325,063 + HMPREF1181_RS14020 0.15 -1.7 325,094 - HMPREF1181_RS14020 0.18 -1.3 325,229 - HMPREF1181_RS14020 0.32 +0.1 325,503 + HMPREF1181_RS14020 0.59 -1.4 325,507 + HMPREF1181_RS14020 0.59 -2.4 325,741 - HMPREF1181_RS14020 0.82 +0.1 325,899 - -0.9 325,905 + -0.7 325,919 + +0.4 325,962 + +0.3 326,092 + -2.7 326,675 - HMPREF1181_RS14025 0.42 -0.7 326,697 - HMPREF1181_RS14025 0.43 -1.6 326,816 + HMPREF1181_RS14025 0.50 +0.2 326,816 + HMPREF1181_RS14025 0.50 -1.6 326,865 - HMPREF1181_RS14025 0.53 -1.1 327,030 - HMPREF1181_RS14025 0.63 +0.3 327,178 + HMPREF1181_RS14025 0.71 -0.4 327,585 - +0.3 327,590 - -1.1 327,631 - -2.3 327,672 + -0.3 327,731 + +0.1 327,744 - -1.8 327,768 + -0.4 327,768 + +0.2 327,768 + +0.2 327,773 - -0.4 327,831 + HMPREF1181_RS14030 0.12 +0.1 327,859 + HMPREF1181_RS14030 0.18 +0.2 327,864 + HMPREF1181_RS14030 0.19 -0.2 327,880 + HMPREF1181_RS14030 0.22 +0.1 327,880 + HMPREF1181_RS14030 0.22 +0.6 327,880 + HMPREF1181_RS14030 0.22 +0.1 327,881 - HMPREF1181_RS14030 0.22 -0.0 327,900 + HMPREF1181_RS14030 0.26 -0.1 327,931 + HMPREF1181_RS14030 0.32 -0.1 327,931 + HMPREF1181_RS14030 0.32 +1.1 327,931 + HMPREF1181_RS14030 0.32 -1.3 327,994 + HMPREF1181_RS14030 0.44 -0.2 327,994 + HMPREF1181_RS14030 0.44 -0.1 328,030 + HMPREF1181_RS14030 0.51 -0.9 328,030 + HMPREF1181_RS14030 0.51 +0.3 328,030 + HMPREF1181_RS14030 0.51 +0.5 328,031 - HMPREF1181_RS14030 0.51 -0.4 328,036 + HMPREF1181_RS14030 0.52 -1.3 328,036 + HMPREF1181_RS14030 0.52 -1.8 328,037 - HMPREF1181_RS14030 0.52 +0.0 328,037 - HMPREF1181_RS14030 0.52 -0.3 328,041 + HMPREF1181_RS14030 0.53 -0.8 328,068 + HMPREF1181_RS14030 0.58 -0.3 328,069 - HMPREF1181_RS14030 0.58 +0.1 328,121 + HMPREF1181_RS14030 0.68 +1.1 328,122 - HMPREF1181_RS14030 0.68 +0.0 328,163 + HMPREF1181_RS14030 0.76 +0.9 328,170 + HMPREF1181_RS14030 0.78 +0.4 328,171 - HMPREF1181_RS14030 0.78 -0.8 328,230 + HMPREF1181_RS14030 0.89 +0.1 328,242 + +0.4 328,269 - -0.6 328,310 - -0.0 328,364 - +0.0 328,400 - -0.6 328,426 - -0.2 328,437 + +0.7 328,468 + HMPREF1181_RS14035 0.11 -0.3 328,469 - HMPREF1181_RS14035 0.11 -0.6 328,496 - HMPREF1181_RS14035 0.20 +1.7 328,563 + HMPREF1181_RS14035 0.43 -0.5 328,563 + HMPREF1181_RS14035 0.43 -0.4 328,568 - HMPREF1181_RS14035 0.44 -0.3 328,641 - HMPREF1181_RS14035 0.69 +0.1 328,641 - HMPREF1181_RS14035 0.69 +0.2
Or see this region's nucleotide sequence