Strain Fitness in Escherichia coli HS(pFamp)R (ATCC 700891) around OHPLBJKB_00032

Experiment: K5

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOHPLBJKB_00029 and OHPLBJKB_00030 are separated by 3 nucleotidesOHPLBJKB_00030 and OHPLBJKB_00031 are separated by 74 nucleotidesOHPLBJKB_00031 and OHPLBJKB_00032 overlap by 1 nucleotidesOHPLBJKB_00032 and OHPLBJKB_00033 are separated by 9 nucleotides OHPLBJKB_00029: OHPLBJKB_00029 - Acetolactate synthase isozyme 1 large subunit, at 28,536 to 30,224 _00029 OHPLBJKB_00030: OHPLBJKB_00030 - Acetolactate synthase isozyme 1 small subunit, at 30,228 to 30,518 _00030 OHPLBJKB_00031: OHPLBJKB_00031 - Transcriptional regulatory protein UhpA, at 30,593 to 31,183 _00031 OHPLBJKB_00032: OHPLBJKB_00032 - Signal transduction histidine-protein kinase/phosphatase UhpB, at 31,183 to 32,685 _00032 OHPLBJKB_00033: OHPLBJKB_00033 - Membrane sensor protein UhpC, at 32,695 to 34,014 _00033 Position (kb) 31 32 33Strain fitness (log2 ratio) -2 -1 0 1at 30.223 kb on - strandat 30.253 kb on + strandat 30.253 kb on + strandat 30.254 kb on - strandat 30.381 kb on - strand, within OHPLBJKB_00030at 30.381 kb on - strand, within OHPLBJKB_00030at 30.467 kb on + strand, within OHPLBJKB_00030at 30.468 kb on - strand, within OHPLBJKB_00030at 30.558 kb on + strandat 30.558 kb on + strandat 30.559 kb on - strandat 30.559 kb on - strandat 30.559 kb on - strandat 30.610 kb on + strandat 30.610 kb on + strandat 30.610 kb on + strandat 30.611 kb on - strandat 30.612 kb on + strandat 30.612 kb on + strandat 30.613 kb on - strandat 30.678 kb on + strand, within OHPLBJKB_00031at 30.678 kb on + strand, within OHPLBJKB_00031at 30.678 kb on + strand, within OHPLBJKB_00031at 30.678 kb on + strand, within OHPLBJKB_00031at 30.679 kb on - strand, within OHPLBJKB_00031at 30.679 kb on - strand, within OHPLBJKB_00031at 30.679 kb on - strand, within OHPLBJKB_00031at 30.679 kb on - strand, within OHPLBJKB_00031at 30.745 kb on + strand, within OHPLBJKB_00031at 30.745 kb on + strand, within OHPLBJKB_00031at 30.745 kb on + strand, within OHPLBJKB_00031at 30.746 kb on - strand, within OHPLBJKB_00031at 30.789 kb on + strand, within OHPLBJKB_00031at 30.790 kb on - strand, within OHPLBJKB_00031at 30.808 kb on + strand, within OHPLBJKB_00031at 30.820 kb on + strand, within OHPLBJKB_00031at 30.820 kb on + strand, within OHPLBJKB_00031at 30.932 kb on - strand, within OHPLBJKB_00031at 30.932 kb on - strand, within OHPLBJKB_00031at 30.953 kb on + strand, within OHPLBJKB_00031at 30.976 kb on + strand, within OHPLBJKB_00031at 30.977 kb on - strand, within OHPLBJKB_00031at 30.977 kb on - strand, within OHPLBJKB_00031at 31.099 kb on - strand, within OHPLBJKB_00031at 31.150 kb on - strandat 31.205 kb on + strandat 31.205 kb on + strandat 31.205 kb on + strandat 31.205 kb on + strandat 31.205 kb on + strandat 31.206 kb on - strandat 31.206 kb on - strandat 31.206 kb on - strandat 31.206 kb on - strandat 31.263 kb on + strandat 31.308 kb on - strandat 31.397 kb on - strand, within OHPLBJKB_00032at 31.643 kb on + strand, within OHPLBJKB_00032at 31.643 kb on + strand, within OHPLBJKB_00032at 31.663 kb on + strand, within OHPLBJKB_00032at 31.664 kb on - strand, within OHPLBJKB_00032at 31.675 kb on - strand, within OHPLBJKB_00032at 31.747 kb on + strand, within OHPLBJKB_00032at 31.747 kb on + strand, within OHPLBJKB_00032at 31.858 kb on + strand, within OHPLBJKB_00032at 32.019 kb on + strand, within OHPLBJKB_00032at 32.019 kb on + strand, within OHPLBJKB_00032at 32.019 kb on + strand, within OHPLBJKB_00032at 32.148 kb on + strand, within OHPLBJKB_00032at 32.154 kb on + strand, within OHPLBJKB_00032at 32.231 kb on + strand, within OHPLBJKB_00032at 32.276 kb on + strand, within OHPLBJKB_00032at 32.625 kb on - strandat 32.666 kb on + strandat 32.667 kb on - strandat 32.667 kb on - strandat 32.667 kb on - strandat 32.667 kb on - strandat 32.677 kb on + strandat 32.677 kb on + strandat 32.678 kb on - strandat 32.678 kb on - strandat 32.678 kb on - strandat 32.733 kb on - strandat 32.742 kb on + strandat 32.746 kb on + strandat 32.785 kb on - strandat 32.822 kb on - strandat 32.822 kb on - strandat 32.917 kb on + strand, within OHPLBJKB_00033at 32.917 kb on + strand, within OHPLBJKB_00033at 32.917 kb on + strand, within OHPLBJKB_00033at 32.917 kb on + strand, within OHPLBJKB_00033at 32.922 kb on + strand, within OHPLBJKB_00033at 32.923 kb on - strand, within OHPLBJKB_00033at 32.923 kb on - strand, within OHPLBJKB_00033at 32.923 kb on - strand, within OHPLBJKB_00033at 32.923 kb on - strand, within OHPLBJKB_00033at 32.923 kb on - strand, within OHPLBJKB_00033at 32.926 kb on + strand, within OHPLBJKB_00033at 32.926 kb on + strand, within OHPLBJKB_00033at 32.927 kb on - strand, within OHPLBJKB_00033at 32.927 kb on - strand, within OHPLBJKB_00033at 32.986 kb on - strand, within OHPLBJKB_00033at 33.000 kb on + strand, within OHPLBJKB_00033at 33.016 kb on - strand, within OHPLBJKB_00033at 33.063 kb on - strand, within OHPLBJKB_00033at 33.133 kb on + strand, within OHPLBJKB_00033at 33.231 kb on - strand, within OHPLBJKB_00033at 33.270 kb on + strand, within OHPLBJKB_00033at 33.271 kb on - strand, within OHPLBJKB_00033at 33.329 kb on + strand, within OHPLBJKB_00033at 33.427 kb on + strand, within OHPLBJKB_00033at 33.427 kb on + strand, within OHPLBJKB_00033at 33.427 kb on + strand, within OHPLBJKB_00033at 33.427 kb on + strand, within OHPLBJKB_00033at 33.428 kb on - strand, within OHPLBJKB_00033at 33.428 kb on - strand, within OHPLBJKB_00033at 33.428 kb on - strand, within OHPLBJKB_00033at 33.445 kb on + strand, within OHPLBJKB_00033at 33.446 kb on - strand, within OHPLBJKB_00033at 33.446 kb on - strand, within OHPLBJKB_00033at 33.447 kb on + strand, within OHPLBJKB_00033at 33.448 kb on - strand, within OHPLBJKB_00033at 33.469 kb on + strand, within OHPLBJKB_00033at 33.469 kb on + strand, within OHPLBJKB_00033at 33.470 kb on - strand, within OHPLBJKB_00033at 33.470 kb on - strand, within OHPLBJKB_00033at 33.470 kb on - strand, within OHPLBJKB_00033at 33.481 kb on + strand, within OHPLBJKB_00033at 33.481 kb on + strand, within OHPLBJKB_00033at 33.481 kb on + strand, within OHPLBJKB_00033at 33.481 kb on + strand, within OHPLBJKB_00033at 33.481 kb on + strand, within OHPLBJKB_00033at 33.482 kb on - strand, within OHPLBJKB_00033at 33.482 kb on - strand, within OHPLBJKB_00033at 33.482 kb on - strand, within OHPLBJKB_00033at 33.482 kb on - strand, within OHPLBJKB_00033at 33.482 kb on - strand, within OHPLBJKB_00033at 33.520 kb on + strand, within OHPLBJKB_00033at 33.520 kb on + strand, within OHPLBJKB_00033at 33.520 kb on + strand, within OHPLBJKB_00033at 33.520 kb on + strand, within OHPLBJKB_00033at 33.520 kb on + strand, within OHPLBJKB_00033at 33.521 kb on - strand, within OHPLBJKB_00033at 33.521 kb on - strand, within OHPLBJKB_00033at 33.521 kb on - strand, within OHPLBJKB_00033at 33.521 kb on - strand, within OHPLBJKB_00033at 33.521 kb on - strand, within OHPLBJKB_00033at 33.522 kb on + strand, within OHPLBJKB_00033at 33.522 kb on + strand, within OHPLBJKB_00033at 33.523 kb on - strand, within OHPLBJKB_00033at 33.523 kb on - strand, within OHPLBJKB_00033at 33.523 kb on - strand, within OHPLBJKB_00033at 33.523 kb on - strand, within OHPLBJKB_00033

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Per-strain Table

Position Strand Gene LocusTag Fraction K5
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30,223 - -1.0
30,253 + -2.1
30,253 + +1.0
30,254 - +1.3
30,381 - OHPLBJKB_00030 0.53 -1.5
30,381 - OHPLBJKB_00030 0.53 +0.3
30,467 + OHPLBJKB_00030 0.82 +0.7
30,468 - OHPLBJKB_00030 0.82 -2.1
30,558 + +0.2
30,558 + +0.1
30,559 - -0.9
30,559 - -0.5
30,559 - +0.1
30,610 + -0.8
30,610 + +0.4
30,610 + +0.2
30,611 - -0.4
30,612 + -0.3
30,612 + -0.5
30,613 - -0.1
30,678 + OHPLBJKB_00031 0.14 +1.4
30,678 + OHPLBJKB_00031 0.14 -0.6
30,678 + OHPLBJKB_00031 0.14 +0.0
30,678 + OHPLBJKB_00031 0.14 -0.0
30,679 - OHPLBJKB_00031 0.15 +0.1
30,679 - OHPLBJKB_00031 0.15 +1.1
30,679 - OHPLBJKB_00031 0.15 -0.2
30,679 - OHPLBJKB_00031 0.15 -0.1
30,745 + OHPLBJKB_00031 0.26 -0.3
30,745 + OHPLBJKB_00031 0.26 +0.1
30,745 + OHPLBJKB_00031 0.26 -0.8
30,746 - OHPLBJKB_00031 0.26 +0.2
30,789 + OHPLBJKB_00031 0.33 -0.3
30,790 - OHPLBJKB_00031 0.33 -0.5
30,808 + OHPLBJKB_00031 0.36 +0.1
30,820 + OHPLBJKB_00031 0.38 +0.3
30,820 + OHPLBJKB_00031 0.38 -0.7
30,932 - OHPLBJKB_00031 0.57 +0.7
30,932 - OHPLBJKB_00031 0.57 +0.4
30,953 + OHPLBJKB_00031 0.61 +0.5
30,976 + OHPLBJKB_00031 0.65 -0.4
30,977 - OHPLBJKB_00031 0.65 +1.0
30,977 - OHPLBJKB_00031 0.65 +1.4
31,099 - OHPLBJKB_00031 0.86 +0.6
31,150 - -2.7
31,205 + +0.3
31,205 + -0.1
31,205 + +1.6
31,205 + +0.1
31,205 + +0.5
31,206 - +0.0
31,206 - +0.9
31,206 - +1.5
31,206 - +0.3
31,263 + -0.3
31,308 - -0.2
31,397 - OHPLBJKB_00032 0.14 -0.0
31,643 + OHPLBJKB_00032 0.31 +1.7
31,643 + OHPLBJKB_00032 0.31 -0.5
31,663 + OHPLBJKB_00032 0.32 +0.6
31,664 - OHPLBJKB_00032 0.32 +0.3
31,675 - OHPLBJKB_00032 0.33 +1.1
31,747 + OHPLBJKB_00032 0.38 +0.7
31,747 + OHPLBJKB_00032 0.38 -0.4
31,858 + OHPLBJKB_00032 0.45 +0.8
32,019 + OHPLBJKB_00032 0.56 -0.0
32,019 + OHPLBJKB_00032 0.56 -0.8
32,019 + OHPLBJKB_00032 0.56 -1.0
32,148 + OHPLBJKB_00032 0.64 +0.2
32,154 + OHPLBJKB_00032 0.65 +0.9
32,231 + OHPLBJKB_00032 0.70 -0.3
32,276 + OHPLBJKB_00032 0.73 +1.5
32,625 - -1.3
32,666 + +0.6
32,667 - -0.5
32,667 - -0.2
32,667 - -0.7
32,667 - -0.9
32,677 + -0.3
32,677 + -0.5
32,678 - -0.0
32,678 - -0.5
32,678 - +0.4
32,733 - +0.9
32,742 + +0.5
32,746 + -0.2
32,785 - +0.4
32,822 - -0.4
32,822 - -0.3
32,917 + OHPLBJKB_00033 0.17 +0.1
32,917 + OHPLBJKB_00033 0.17 -0.5
32,917 + OHPLBJKB_00033 0.17 -0.6
32,917 + OHPLBJKB_00033 0.17 -0.6
32,922 + OHPLBJKB_00033 0.17 -0.2
32,923 - OHPLBJKB_00033 0.17 +0.2
32,923 - OHPLBJKB_00033 0.17 +1.2
32,923 - OHPLBJKB_00033 0.17 +0.1
32,923 - OHPLBJKB_00033 0.17 +1.0
32,923 - OHPLBJKB_00033 0.17 -0.2
32,926 + OHPLBJKB_00033 0.17 -0.5
32,926 + OHPLBJKB_00033 0.17 -0.1
32,927 - OHPLBJKB_00033 0.18 +0.5
32,927 - OHPLBJKB_00033 0.18 -0.6
32,986 - OHPLBJKB_00033 0.22 -0.2
33,000 + OHPLBJKB_00033 0.23 -1.6
33,016 - OHPLBJKB_00033 0.24 -0.8
33,063 - OHPLBJKB_00033 0.28 -0.0
33,133 + OHPLBJKB_00033 0.33 +0.6
33,231 - OHPLBJKB_00033 0.41 -0.5
33,270 + OHPLBJKB_00033 0.44 +1.2
33,271 - OHPLBJKB_00033 0.44 +1.1
33,329 + OHPLBJKB_00033 0.48 -0.3
33,427 + OHPLBJKB_00033 0.55 +1.0
33,427 + OHPLBJKB_00033 0.55 -0.7
33,427 + OHPLBJKB_00033 0.55 +0.1
33,427 + OHPLBJKB_00033 0.55 +0.0
33,428 - OHPLBJKB_00033 0.56 -1.2
33,428 - OHPLBJKB_00033 0.56 +1.2
33,428 - OHPLBJKB_00033 0.56 +1.0
33,445 + OHPLBJKB_00033 0.57 +0.7
33,446 - OHPLBJKB_00033 0.57 -0.2
33,446 - OHPLBJKB_00033 0.57 -0.4
33,447 + OHPLBJKB_00033 0.57 -1.5
33,448 - OHPLBJKB_00033 0.57 -1.3
33,469 + OHPLBJKB_00033 0.59 -0.3
33,469 + OHPLBJKB_00033 0.59 -0.4
33,470 - OHPLBJKB_00033 0.59 -1.1
33,470 - OHPLBJKB_00033 0.59 -0.2
33,470 - OHPLBJKB_00033 0.59 -0.0
33,481 + OHPLBJKB_00033 0.60 -0.7
33,481 + OHPLBJKB_00033 0.60 +0.9
33,481 + OHPLBJKB_00033 0.60 -1.7
33,481 + OHPLBJKB_00033 0.60 +0.1
33,481 + OHPLBJKB_00033 0.60 -0.3
33,482 - OHPLBJKB_00033 0.60 -0.1
33,482 - OHPLBJKB_00033 0.60 -1.3
33,482 - OHPLBJKB_00033 0.60 -0.6
33,482 - OHPLBJKB_00033 0.60 -0.0
33,482 - OHPLBJKB_00033 0.60 +0.3
33,520 + OHPLBJKB_00033 0.62 -1.5
33,520 + OHPLBJKB_00033 0.62 -1.0
33,520 + OHPLBJKB_00033 0.62 -1.4
33,520 + OHPLBJKB_00033 0.62 +0.8
33,520 + OHPLBJKB_00033 0.62 -0.1
33,521 - OHPLBJKB_00033 0.63 +0.6
33,521 - OHPLBJKB_00033 0.63 -0.2
33,521 - OHPLBJKB_00033 0.63 -0.6
33,521 - OHPLBJKB_00033 0.63 +1.0
33,521 - OHPLBJKB_00033 0.63 +0.2
33,522 + OHPLBJKB_00033 0.63 -0.1
33,522 + OHPLBJKB_00033 0.63 +0.3
33,523 - OHPLBJKB_00033 0.63 +0.7
33,523 - OHPLBJKB_00033 0.63 -0.6
33,523 - OHPLBJKB_00033 0.63 +1.1
33,523 - OHPLBJKB_00033 0.63 +0.6

Or see this region's nucleotide sequence