Experiment: Experiment:TE2, Cucumber 1 Petiole 2 (TE2-S-C1-P2)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gloB and LU632_RS01085 are separated by 45 nucleotides LU632_RS01085 and rnhA overlap by 16 nucleotides rnhA and dnaQ are separated by 51 nucleotides dnaQ and LU632_RS01100 are separated by 122 nucleotides
LU632_RS01080: gloB - hydroxyacylglutathione hydrolase, at 233,072 to 233,827
gloB
LU632_RS01085: LU632_RS01085 - class I SAM-dependent methyltransferase, at 233,873 to 234,610
_RS01085
LU632_RS01090: rnhA - ribonuclease HI, at 234,595 to 235,062
rnhA
LU632_RS01095: dnaQ - DNA polymerase III subunit epsilon, at 235,114 to 235,848
dnaQ
LU632_RS01100: LU632_RS01100 - tRNA-Asp, at 235,971 to 236,047
_RS01100
Position (kb) 234
235
236 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 233.604 kb on + strand, within gloB at 233.711 kb on + strand, within gloB at 233.790 kb on + strand at 233.790 kb on + strand at 233.791 kb on - strand at 233.791 kb on - strand at 233.791 kb on - strand at 233.819 kb on - strand at 233.850 kb on - strand at 233.851 kb on + strand at 234.055 kb on + strand, within LU632_RS01085 at 234.055 kb on + strand, within LU632_RS01085 at 234.370 kb on + strand, within LU632_RS01085 at 234.370 kb on + strand, within LU632_RS01085 at 234.371 kb on - strand, within LU632_RS01085 at 234.375 kb on - strand, within LU632_RS01085 at 234.628 kb on + strand at 234.659 kb on - strand, within rnhA at 234.845 kb on + strand, within rnhA at 234.846 kb on - strand, within rnhA at 234.866 kb on - strand, within rnhA at 235.102 kb on + strand at 235.850 kb on + strand at 235.851 kb on - strand at 235.889 kb on + strand at 235.889 kb on + strand at 235.890 kb on - strand at 235.938 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Cucumber 1 Petiole 2 (TE2-S-C1-P2) remove 233,604 + gloB LU632_RS01080 0.70 -0.6 233,711 + gloB LU632_RS01080 0.85 -2.6 233,790 + -1.9 233,790 + +2.3 233,791 - -0.4 233,791 - -0.2 233,791 - +0.8 233,819 - +1.8 233,850 - +0.8 233,851 + -2.1 234,055 + LU632_RS01085 0.25 +0.3 234,055 + LU632_RS01085 0.25 -3.2 234,370 + LU632_RS01085 0.67 -1.5 234,370 + LU632_RS01085 0.67 -2.6 234,371 - LU632_RS01085 0.67 -0.5 234,375 - LU632_RS01085 0.68 -1.6 234,628 + -1.9 234,659 - rnhA LU632_RS01090 0.14 -2.4 234,845 + rnhA LU632_RS01090 0.53 -1.6 234,846 - rnhA LU632_RS01090 0.54 -1.8 234,866 - rnhA LU632_RS01090 0.58 -0.8 235,102 + +0.2 235,850 + -0.2 235,851 - +2.8 235,889 + -1.0 235,889 + +1.9 235,890 - +1.6 235,938 - -1.9
Or see this region's nucleotide sequence