Experiment: Experiment:TE2, Melon 5 Petiole 2 (TE2-S-M5-P2)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinB and pepD are separated by 167 nucleotides pepD and gpt are separated by 252 nucleotides gpt and frsA are separated by 131 nucleotides
LU632_RS01150: dinB - DNA polymerase IV, at 244,193 to 245,248
dinB
LU632_RS01155: pepD - beta-Ala-His dipeptidase, at 245,416 to 246,873
pepD
LU632_RS01160: gpt - xanthine phosphoribosyltransferase, at 247,126 to 247,584
gpt
LU632_RS01165: frsA - esterase FrsA, at 247,716 to 248,948
frsA
Position (kb) 245
246
247 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 244.427 kb on + strand, within dinB at 244.428 kb on - strand, within dinB at 244.429 kb on + strand, within dinB at 244.430 kb on - strand, within dinB at 244.448 kb on - strand, within dinB at 244.508 kb on + strand, within dinB at 244.508 kb on + strand, within dinB at 244.509 kb on - strand, within dinB at 244.518 kb on + strand, within dinB at 244.518 kb on + strand, within dinB at 244.519 kb on - strand, within dinB at 244.519 kb on - strand, within dinB at 244.539 kb on - strand, within dinB at 244.580 kb on - strand, within dinB at 244.948 kb on + strand, within dinB at 245.056 kb on - strand, within dinB at 245.430 kb on + strand at 245.430 kb on + strand at 245.431 kb on - strand at 245.431 kb on - strand at 245.462 kb on + strand at 245.481 kb on + strand at 245.481 kb on + strand at 245.485 kb on + strand at 245.485 kb on + strand at 245.485 kb on + strand at 245.486 kb on - strand at 245.486 kb on - strand at 245.486 kb on - strand at 245.486 kb on - strand at 245.486 kb on - strand at 245.563 kb on - strand, within pepD at 245.613 kb on + strand, within pepD at 245.753 kb on + strand, within pepD at 245.753 kb on + strand, within pepD at 245.753 kb on + strand, within pepD at 245.794 kb on - strand, within pepD at 246.157 kb on + strand, within pepD at 246.157 kb on + strand, within pepD at 246.157 kb on + strand, within pepD at 246.189 kb on + strand, within pepD at 246.216 kb on + strand, within pepD at 246.216 kb on + strand, within pepD at 246.217 kb on - strand, within pepD at 246.270 kb on + strand, within pepD at 246.271 kb on - strand, within pepD at 246.344 kb on + strand, within pepD at 246.344 kb on + strand, within pepD at 246.344 kb on + strand, within pepD at 246.344 kb on + strand, within pepD at 246.345 kb on - strand, within pepD at 246.345 kb on - strand, within pepD at 246.345 kb on - strand, within pepD at 246.345 kb on - strand, within pepD at 246.345 kb on - strand, within pepD at 246.345 kb on - strand, within pepD at 246.601 kb on - strand, within pepD at 246.610 kb on + strand, within pepD at 246.610 kb on + strand, within pepD at 246.611 kb on - strand, within pepD at 246.712 kb on - strand, within pepD at 246.723 kb on + strand, within pepD at 246.724 kb on - strand, within pepD at 246.768 kb on + strand at 246.768 kb on + strand at 246.771 kb on - strand at 246.771 kb on - strand at 246.876 kb on + strand at 246.887 kb on + strand at 246.888 kb on - strand at 246.982 kb on - strand at 246.999 kb on - strand at 247.061 kb on + strand at 247.229 kb on + strand, within gpt at 247.298 kb on + strand, within gpt at 247.435 kb on + strand, within gpt at 247.560 kb on + strand at 247.667 kb on + strand at 247.667 kb on + strand at 247.667 kb on - strand at 247.697 kb on + strand at 247.698 kb on - strand at 247.702 kb on + strand at 247.703 kb on - strand at 247.703 kb on - strand at 247.734 kb on - strand at 247.806 kb on + strand at 247.806 kb on + strand at 247.807 kb on - strand at 247.862 kb on - strand, within frsA
Per-strain Table
Position Strand Gene LocusTag Fraction Experiment:TE2, Melon 5 Petiole 2 (TE2-S-M5-P2) remove 244,427 + dinB LU632_RS01150 0.22 -0.4 244,428 - dinB LU632_RS01150 0.22 -0.1 244,429 + dinB LU632_RS01150 0.22 -3.0 244,430 - dinB LU632_RS01150 0.22 +1.2 244,448 - dinB LU632_RS01150 0.24 -0.6 244,508 + dinB LU632_RS01150 0.30 -0.6 244,508 + dinB LU632_RS01150 0.30 +0.2 244,509 - dinB LU632_RS01150 0.30 -0.3 244,518 + dinB LU632_RS01150 0.31 +0.2 244,518 + dinB LU632_RS01150 0.31 -0.1 244,519 - dinB LU632_RS01150 0.31 +0.0 244,519 - dinB LU632_RS01150 0.31 +1.2 244,539 - dinB LU632_RS01150 0.33 -0.3 244,580 - dinB LU632_RS01150 0.37 +1.2 244,948 + dinB LU632_RS01150 0.71 +0.1 245,056 - dinB LU632_RS01150 0.82 +0.0 245,430 + -0.3 245,430 + +0.1 245,431 - +0.3 245,431 - -1.0 245,462 + +0.9 245,481 + +0.6 245,481 + -1.0 245,485 + +1.2 245,485 + -0.6 245,485 + -2.9 245,486 - +0.2 245,486 - +0.1 245,486 - -0.6 245,486 - -2.0 245,486 - -1.2 245,563 - pepD LU632_RS01155 0.10 -0.5 245,613 + pepD LU632_RS01155 0.14 -1.6 245,753 + pepD LU632_RS01155 0.23 +0.1 245,753 + pepD LU632_RS01155 0.23 +1.2 245,753 + pepD LU632_RS01155 0.23 -0.7 245,794 - pepD LU632_RS01155 0.26 -1.0 246,157 + pepD LU632_RS01155 0.51 +1.6 246,157 + pepD LU632_RS01155 0.51 -0.6 246,157 + pepD LU632_RS01155 0.51 +0.4 246,189 + pepD LU632_RS01155 0.53 +1.2 246,216 + pepD LU632_RS01155 0.55 -1.4 246,216 + pepD LU632_RS01155 0.55 +3.6 246,217 - pepD LU632_RS01155 0.55 +0.7 246,270 + pepD LU632_RS01155 0.59 -0.7 246,271 - pepD LU632_RS01155 0.59 -2.3 246,344 + pepD LU632_RS01155 0.64 +0.1 246,344 + pepD LU632_RS01155 0.64 +1.6 246,344 + pepD LU632_RS01155 0.64 -1.1 246,344 + pepD LU632_RS01155 0.64 -0.1 246,345 - pepD LU632_RS01155 0.64 +0.0 246,345 - pepD LU632_RS01155 0.64 +0.3 246,345 - pepD LU632_RS01155 0.64 -1.5 246,345 - pepD LU632_RS01155 0.64 -0.0 246,345 - pepD LU632_RS01155 0.64 +2.8 246,345 - pepD LU632_RS01155 0.64 -0.7 246,601 - pepD LU632_RS01155 0.81 -1.6 246,610 + pepD LU632_RS01155 0.82 +0.2 246,610 + pepD LU632_RS01155 0.82 -2.0 246,611 - pepD LU632_RS01155 0.82 +0.2 246,712 - pepD LU632_RS01155 0.89 -0.8 246,723 + pepD LU632_RS01155 0.90 -1.6 246,724 - pepD LU632_RS01155 0.90 -1.8 246,768 + -1.2 246,768 + +1.4 246,771 - -2.4 246,771 - -0.8 246,876 + -1.6 246,887 + +1.2 246,888 - +1.2 246,982 - +1.0 246,999 - -1.6 247,061 + -0.8 247,229 + gpt LU632_RS01160 0.22 +2.2 247,298 + gpt LU632_RS01160 0.37 -1.1 247,435 + gpt LU632_RS01160 0.67 +0.2 247,560 + +3.2 247,667 + -1.1 247,667 + -2.5 247,667 - -0.2 247,697 + +0.2 247,698 - +0.8 247,702 + +1.1 247,703 - -0.1 247,703 - +3.2 247,734 - +0.2 247,806 + -0.8 247,806 + +1.4 247,807 - -0.1 247,862 - frsA LU632_RS01165 0.12 +0.9
Or see this region's nucleotide sequence