Experiment: Yeast Extract 2.5 mg/mL (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS02210 and HMPREF1181_RS02215 are separated by 107 nucleotides HMPREF1181_RS02215 and HMPREF1181_RS17735 are separated by 120 nucleotides HMPREF1181_RS17735 and HMPREF1181_RS17190 overlap by 42 nucleotides
HMPREF1181_RS02210: HMPREF1181_RS02210 - mannonate dehydratase, at 542,264 to 543,436
_RS02210
HMPREF1181_RS02215: HMPREF1181_RS02215 - SDR family oxidoreductase, at 543,544 to 544,353
_RS02215
HMPREF1181_RS17735: HMPREF1181_RS17735 - IS982 family transposase, at 544,474 to 544,944
_RS17735
HMPREF1181_RS17190: HMPREF1181_RS17190 - epoxyqueuosine reductase QueH, at 544,903 to 545,334
_RS17190
Position (kb)
543
544
545 Strain fitness (log2 ratio)
-2
-1
0
1 at 542.545 kb on + strand, within HMPREF1181_RS02210 at 542.787 kb on + strand, within HMPREF1181_RS02210 at 542.788 kb on - strand, within HMPREF1181_RS02210 at 542.790 kb on - strand, within HMPREF1181_RS02210 at 542.790 kb on - strand, within HMPREF1181_RS02210 at 542.864 kb on - strand, within HMPREF1181_RS02210 at 543.124 kb on - strand, within HMPREF1181_RS02210 at 543.186 kb on + strand, within HMPREF1181_RS02210 at 543.186 kb on + strand, within HMPREF1181_RS02210 at 543.228 kb on - strand, within HMPREF1181_RS02210 at 543.431 kb on + strand at 543.441 kb on + strand at 543.442 kb on - strand at 543.442 kb on - strand at 543.447 kb on + strand at 543.449 kb on + strand at 543.458 kb on - strand at 543.474 kb on + strand at 543.500 kb on - strand at 543.500 kb on - strand at 543.707 kb on + strand, within HMPREF1181_RS02215 at 543.822 kb on + strand, within HMPREF1181_RS02215 at 543.824 kb on + strand, within HMPREF1181_RS02215 at 543.825 kb on + strand, within HMPREF1181_RS02215 at 543.832 kb on - strand, within HMPREF1181_RS02215 at 543.832 kb on - strand, within HMPREF1181_RS02215 at 543.833 kb on + strand, within HMPREF1181_RS02215 at 543.834 kb on - strand, within HMPREF1181_RS02215 at 543.834 kb on - strand, within HMPREF1181_RS02215 at 543.868 kb on + strand, within HMPREF1181_RS02215 at 544.272 kb on - strand, within HMPREF1181_RS02215 at 544.322 kb on - strand at 544.358 kb on + strand at 544.359 kb on - strand at 544.366 kb on + strand at 544.366 kb on + strand at 544.395 kb on - strand at 544.504 kb on + strand at 544.505 kb on - strand at 544.562 kb on + strand, within HMPREF1181_RS17735 at 544.572 kb on + strand, within HMPREF1181_RS17735 at 544.620 kb on + strand, within HMPREF1181_RS17735 at 544.621 kb on - strand, within HMPREF1181_RS17735 at 544.621 kb on - strand, within HMPREF1181_RS17735 at 544.623 kb on - strand, within HMPREF1181_RS17735 at 544.632 kb on + strand, within HMPREF1181_RS17735 at 544.633 kb on - strand, within HMPREF1181_RS17735 at 544.675 kb on + strand, within HMPREF1181_RS17735 at 544.675 kb on + strand, within HMPREF1181_RS17735 at 544.725 kb on + strand, within HMPREF1181_RS17735 at 544.822 kb on - strand, within HMPREF1181_RS17735 at 544.865 kb on + strand, within HMPREF1181_RS17735 at 544.892 kb on + strand, within HMPREF1181_RS17735 at 544.892 kb on + strand, within HMPREF1181_RS17735 at 544.892 kb on + strand, within HMPREF1181_RS17735 at 544.893 kb on - strand, within HMPREF1181_RS17735 at 544.893 kb on - strand, within HMPREF1181_RS17735 at 544.922 kb on - strand at 544.923 kb on + strand at 544.924 kb on - strand at 545.038 kb on + strand, within HMPREF1181_RS17190 at 545.039 kb on - strand, within HMPREF1181_RS17190 at 545.067 kb on + strand, within HMPREF1181_RS17190 at 545.118 kb on - strand, within HMPREF1181_RS17190 at 545.123 kb on + strand, within HMPREF1181_RS17190 at 545.124 kb on - strand, within HMPREF1181_RS17190 at 545.224 kb on - strand, within HMPREF1181_RS17190 at 545.231 kb on - strand, within HMPREF1181_RS17190 at 545.254 kb on + strand, within HMPREF1181_RS17190 at 545.255 kb on - strand, within HMPREF1181_RS17190
Per-strain Table
Position Strand Gene LocusTag Fraction Yeast Extract 2.5 mg/mL (C) complex media remove 542,545 + HMPREF1181_RS02210 0.24 -0.5 542,787 + HMPREF1181_RS02210 0.45 +0.1 542,788 - HMPREF1181_RS02210 0.45 -0.4 542,790 - HMPREF1181_RS02210 0.45 -0.2 542,790 - HMPREF1181_RS02210 0.45 +1.0 542,864 - HMPREF1181_RS02210 0.51 +0.9 543,124 - HMPREF1181_RS02210 0.73 -0.7 543,186 + HMPREF1181_RS02210 0.79 +1.3 543,186 + HMPREF1181_RS02210 0.79 +0.6 543,228 - HMPREF1181_RS02210 0.82 +0.4 543,431 + +1.0 543,441 + +0.6 543,442 - +0.9 543,442 - -0.1 543,447 + -1.2 543,449 + -0.4 543,458 - +0.1 543,474 + -1.9 543,500 - +0.1 543,500 - +0.5 543,707 + HMPREF1181_RS02215 0.20 +1.5 543,822 + HMPREF1181_RS02215 0.34 -0.1 543,824 + HMPREF1181_RS02215 0.35 +0.1 543,825 + HMPREF1181_RS02215 0.35 -0.1 543,832 - HMPREF1181_RS02215 0.36 -0.4 543,832 - HMPREF1181_RS02215 0.36 -0.9 543,833 + HMPREF1181_RS02215 0.36 -1.6 543,834 - HMPREF1181_RS02215 0.36 +0.2 543,834 - HMPREF1181_RS02215 0.36 -1.3 543,868 + HMPREF1181_RS02215 0.40 +0.6 544,272 - HMPREF1181_RS02215 0.90 +1.1 544,322 - +0.4 544,358 + +1.2 544,359 - +1.4 544,366 + -0.4 544,366 + +0.1 544,395 - -0.2 544,504 + -0.7 544,505 - -0.2 544,562 + HMPREF1181_RS17735 0.19 +0.8 544,572 + HMPREF1181_RS17735 0.21 +0.7 544,620 + HMPREF1181_RS17735 0.31 +0.0 544,621 - HMPREF1181_RS17735 0.31 -1.9 544,621 - HMPREF1181_RS17735 0.31 -0.5 544,623 - HMPREF1181_RS17735 0.32 +0.3 544,632 + HMPREF1181_RS17735 0.34 +0.1 544,633 - HMPREF1181_RS17735 0.34 -1.8 544,675 + HMPREF1181_RS17735 0.43 +1.5 544,675 + HMPREF1181_RS17735 0.43 -0.0 544,725 + HMPREF1181_RS17735 0.53 +0.5 544,822 - HMPREF1181_RS17735 0.74 -0.6 544,865 + HMPREF1181_RS17735 0.83 -0.2 544,892 + HMPREF1181_RS17735 0.89 -0.8 544,892 + HMPREF1181_RS17735 0.89 +0.8 544,892 + HMPREF1181_RS17735 0.89 +0.3 544,893 - HMPREF1181_RS17735 0.89 +0.2 544,893 - HMPREF1181_RS17735 0.89 +1.4 544,922 - +0.1 544,923 + -1.4 544,924 - +0.4 545,038 + HMPREF1181_RS17190 0.31 +0.1 545,039 - HMPREF1181_RS17190 0.31 +0.0 545,067 + HMPREF1181_RS17190 0.38 +0.2 545,118 - HMPREF1181_RS17190 0.50 -1.9 545,123 + HMPREF1181_RS17190 0.51 -0.9 545,124 - HMPREF1181_RS17190 0.51 -0.5 545,224 - HMPREF1181_RS17190 0.74 -0.1 545,231 - HMPREF1181_RS17190 0.76 -1.3 545,254 + HMPREF1181_RS17190 0.81 -0.8 545,255 - HMPREF1181_RS17190 0.81 +0.5
Or see this region's nucleotide sequence