Experiment: Yeast Extract 2.5 mg/mL (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS02080 and HMPREF1181_RS02085 overlap by 11 nucleotides HMPREF1181_RS02085 and HMPREF1181_RS02090 overlap by 1 nucleotides HMPREF1181_RS02090 and HMPREF1181_RS02095 are separated by 91 nucleotides
HMPREF1181_RS02080: HMPREF1181_RS02080 - deoxyribose-phosphate aldolase, at 512,289 to 513,191
_RS02080
HMPREF1181_RS02085: HMPREF1181_RS02085 - nucleotide pyrophosphohydrolase, at 513,181 to 513,516
_RS02085
HMPREF1181_RS02090: HMPREF1181_RS02090 - D-tyrosyl-tRNA(Tyr) deacylase, at 513,516 to 513,968
_RS02090
HMPREF1181_RS02095: HMPREF1181_RS02095 - excinuclease ABC subunit UvrC, at 514,060 to 515,880
_RS02095
Position (kb)
513
514 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 512.587 kb on + strand, within HMPREF1181_RS02080 at 512.716 kb on + strand, within HMPREF1181_RS02080 at 512.748 kb on + strand, within HMPREF1181_RS02080 at 512.748 kb on + strand, within HMPREF1181_RS02080 at 512.758 kb on - strand, within HMPREF1181_RS02080 at 512.800 kb on - strand, within HMPREF1181_RS02080 at 512.879 kb on + strand, within HMPREF1181_RS02080 at 512.890 kb on - strand, within HMPREF1181_RS02080 at 512.910 kb on + strand, within HMPREF1181_RS02080 at 512.910 kb on + strand, within HMPREF1181_RS02080 at 512.911 kb on - strand, within HMPREF1181_RS02080 at 513.006 kb on - strand, within HMPREF1181_RS02080 at 513.139 kb on + strand at 513.140 kb on - strand at 513.161 kb on - strand at 513.161 kb on - strand at 513.204 kb on + strand at 513.216 kb on + strand, within HMPREF1181_RS02085 at 513.216 kb on + strand, within HMPREF1181_RS02085 at 513.217 kb on - strand, within HMPREF1181_RS02085 at 513.380 kb on + strand, within HMPREF1181_RS02085 at 513.460 kb on + strand, within HMPREF1181_RS02085 at 513.461 kb on - strand, within HMPREF1181_RS02085 at 513.461 kb on - strand, within HMPREF1181_RS02085 at 513.461 kb on - strand, within HMPREF1181_RS02085 at 513.518 kb on - strand at 513.573 kb on + strand, within HMPREF1181_RS02090 at 513.573 kb on + strand, within HMPREF1181_RS02090 at 513.582 kb on - strand, within HMPREF1181_RS02090 at 513.602 kb on + strand, within HMPREF1181_RS02090 at 513.607 kb on - strand, within HMPREF1181_RS02090 at 513.607 kb on - strand, within HMPREF1181_RS02090 at 513.691 kb on + strand, within HMPREF1181_RS02090 at 513.691 kb on + strand, within HMPREF1181_RS02090 at 513.692 kb on - strand, within HMPREF1181_RS02090 at 513.732 kb on - strand, within HMPREF1181_RS02090 at 513.779 kb on + strand, within HMPREF1181_RS02090 at 513.780 kb on - strand, within HMPREF1181_RS02090 at 513.786 kb on - strand, within HMPREF1181_RS02090 at 513.871 kb on - strand, within HMPREF1181_RS02090 at 513.887 kb on + strand, within HMPREF1181_RS02090 at 513.888 kb on - strand, within HMPREF1181_RS02090 at 513.954 kb on + strand at 513.955 kb on - strand at 513.957 kb on - strand at 513.960 kb on + strand at 513.961 kb on - strand at 513.973 kb on + strand at 514.061 kb on + strand at 514.228 kb on + strand at 514.229 kb on - strand at 514.279 kb on + strand, within HMPREF1181_RS02095 at 514.279 kb on + strand, within HMPREF1181_RS02095 at 514.292 kb on - strand, within HMPREF1181_RS02095 at 514.479 kb on + strand, within HMPREF1181_RS02095 at 514.479 kb on + strand, within HMPREF1181_RS02095 at 514.534 kb on + strand, within HMPREF1181_RS02095 at 514.534 kb on + strand, within HMPREF1181_RS02095 at 514.535 kb on - strand, within HMPREF1181_RS02095 at 514.588 kb on - strand, within HMPREF1181_RS02095 at 514.589 kb on + strand, within HMPREF1181_RS02095 at 514.589 kb on + strand, within HMPREF1181_RS02095 at 514.589 kb on + strand, within HMPREF1181_RS02095 at 514.799 kb on + strand, within HMPREF1181_RS02095
Per-strain Table
Position Strand Gene LocusTag Fraction Yeast Extract 2.5 mg/mL (C) complex media remove 512,587 + HMPREF1181_RS02080 0.33 -2.1 512,716 + HMPREF1181_RS02080 0.47 -2.9 512,748 + HMPREF1181_RS02080 0.51 -1.6 512,748 + HMPREF1181_RS02080 0.51 -2.0 512,758 - HMPREF1181_RS02080 0.52 -2.5 512,800 - HMPREF1181_RS02080 0.57 +1.0 512,879 + HMPREF1181_RS02080 0.65 -0.6 512,890 - HMPREF1181_RS02080 0.67 -1.8 512,910 + HMPREF1181_RS02080 0.69 -0.6 512,910 + HMPREF1181_RS02080 0.69 -0.2 512,911 - HMPREF1181_RS02080 0.69 +0.5 513,006 - HMPREF1181_RS02080 0.79 -1.3 513,139 + +0.6 513,140 - -0.9 513,161 - -2.0 513,161 - +1.6 513,204 + +0.0 513,216 + HMPREF1181_RS02085 0.10 +0.3 513,216 + HMPREF1181_RS02085 0.10 +0.4 513,217 - HMPREF1181_RS02085 0.11 -0.4 513,380 + HMPREF1181_RS02085 0.59 -0.4 513,460 + HMPREF1181_RS02085 0.83 -0.2 513,461 - HMPREF1181_RS02085 0.83 -0.1 513,461 - HMPREF1181_RS02085 0.83 -2.3 513,461 - HMPREF1181_RS02085 0.83 +0.3 513,518 - +1.3 513,573 + HMPREF1181_RS02090 0.13 +0.2 513,573 + HMPREF1181_RS02090 0.13 +0.6 513,582 - HMPREF1181_RS02090 0.15 -0.8 513,602 + HMPREF1181_RS02090 0.19 +0.7 513,607 - HMPREF1181_RS02090 0.20 +0.2 513,607 - HMPREF1181_RS02090 0.20 +1.1 513,691 + HMPREF1181_RS02090 0.39 -0.9 513,691 + HMPREF1181_RS02090 0.39 -0.6 513,692 - HMPREF1181_RS02090 0.39 +0.9 513,732 - HMPREF1181_RS02090 0.48 +0.6 513,779 + HMPREF1181_RS02090 0.58 -0.1 513,780 - HMPREF1181_RS02090 0.58 +0.0 513,786 - HMPREF1181_RS02090 0.60 +0.9 513,871 - HMPREF1181_RS02090 0.78 +0.9 513,887 + HMPREF1181_RS02090 0.82 -1.1 513,888 - HMPREF1181_RS02090 0.82 +0.4 513,954 + -1.0 513,955 - +0.7 513,957 - -0.3 513,960 + +0.2 513,961 - -0.1 513,973 + +0.4 514,061 + -0.4 514,228 + +0.1 514,229 - -1.1 514,279 + HMPREF1181_RS02095 0.12 -0.6 514,279 + HMPREF1181_RS02095 0.12 -0.6 514,292 - HMPREF1181_RS02095 0.13 +0.3 514,479 + HMPREF1181_RS02095 0.23 -0.9 514,479 + HMPREF1181_RS02095 0.23 -0.2 514,534 + HMPREF1181_RS02095 0.26 -0.1 514,534 + HMPREF1181_RS02095 0.26 -0.4 514,535 - HMPREF1181_RS02095 0.26 +0.8 514,588 - HMPREF1181_RS02095 0.29 +0.6 514,589 + HMPREF1181_RS02095 0.29 +1.0 514,589 + HMPREF1181_RS02095 0.29 +0.7 514,589 + HMPREF1181_RS02095 0.29 -0.1 514,799 + HMPREF1181_RS02095 0.41 -1.7
Or see this region's nucleotide sequence