Experiment: Beta-Lactose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1181_RS14085 and HMPREF1181_RS14090 overlap by 4 nucleotides HMPREF1181_RS14090 and HMPREF1181_RS14095 are separated by 122 nucleotides HMPREF1181_RS14095 and HMPREF1181_RS14100 are separated by 204 nucleotides
HMPREF1181_RS14085: HMPREF1181_RS14085 - adenylosuccinate synthase, at 341,809 to 343,077
_RS14085
HMPREF1181_RS14090: HMPREF1181_RS14090 - transcriptional repressor, at 343,074 to 343,562
_RS14090
HMPREF1181_RS14095: HMPREF1181_RS14095 - DNA alkylation repair protein, at 343,685 to 344,347
_RS14095
HMPREF1181_RS14100: HMPREF1181_RS14100 - dihydrofolate reductase, at 344,552 to 346,600
_RS14100
Position (kb) 343
344
345 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 342.783 kb on + strand, within HMPREF1181_RS14085 at 342.784 kb on - strand, within HMPREF1181_RS14085 at 342.862 kb on + strand, within HMPREF1181_RS14085 at 342.863 kb on - strand, within HMPREF1181_RS14085 at 342.888 kb on + strand, within HMPREF1181_RS14085 at 343.012 kb on + strand at 343.013 kb on - strand at 343.058 kb on - strand at 343.069 kb on + strand at 343.070 kb on - strand at 343.070 kb on - strand at 343.089 kb on - strand at 343.286 kb on - strand, within HMPREF1181_RS14090 at 343.286 kb on - strand, within HMPREF1181_RS14090 at 343.346 kb on + strand, within HMPREF1181_RS14090 at 343.353 kb on + strand, within HMPREF1181_RS14090 at 343.353 kb on + strand, within HMPREF1181_RS14090 at 343.353 kb on + strand, within HMPREF1181_RS14090 at 343.354 kb on - strand, within HMPREF1181_RS14090 at 343.354 kb on - strand, within HMPREF1181_RS14090 at 343.367 kb on - strand, within HMPREF1181_RS14090 at 343.454 kb on - strand, within HMPREF1181_RS14090 at 343.457 kb on + strand, within HMPREF1181_RS14090 at 343.596 kb on + strand at 343.668 kb on - strand at 343.761 kb on - strand, within HMPREF1181_RS14095 at 343.925 kb on + strand, within HMPREF1181_RS14095 at 343.963 kb on + strand, within HMPREF1181_RS14095 at 344.035 kb on + strand, within HMPREF1181_RS14095 at 344.036 kb on - strand, within HMPREF1181_RS14095 at 344.042 kb on - strand, within HMPREF1181_RS14095 at 344.081 kb on + strand, within HMPREF1181_RS14095 at 344.613 kb on + strand at 344.698 kb on + strand at 344.736 kb on - strand at 344.759 kb on + strand, within HMPREF1181_RS14100 at 344.802 kb on + strand, within HMPREF1181_RS14100 at 344.822 kb on - strand, within HMPREF1181_RS14100 at 344.980 kb on + strand, within HMPREF1181_RS14100 at 344.981 kb on - strand, within HMPREF1181_RS14100 at 345.048 kb on + strand, within HMPREF1181_RS14100 at 345.052 kb on + strand, within HMPREF1181_RS14100 at 345.055 kb on - strand, within HMPREF1181_RS14100 at 345.055 kb on - strand, within HMPREF1181_RS14100 at 345.123 kb on + strand, within HMPREF1181_RS14100 at 345.172 kb on - strand, within HMPREF1181_RS14100 at 345.273 kb on + strand, within HMPREF1181_RS14100 at 345.306 kb on + strand, within HMPREF1181_RS14100 at 345.309 kb on - strand, within HMPREF1181_RS14100
Per-strain Table
Position Strand Gene LocusTag Fraction Beta-Lactose 10 mM (C) remove 342,783 + HMPREF1181_RS14085 0.77 -4.1 342,784 - HMPREF1181_RS14085 0.77 -0.0 342,862 + HMPREF1181_RS14085 0.83 -2.2 342,863 - HMPREF1181_RS14085 0.83 -3.3 342,888 + HMPREF1181_RS14085 0.85 -3.2 343,012 + -2.2 343,013 - -2.7 343,058 - -5.3 343,069 + -0.9 343,070 - -3.4 343,070 - -5.9 343,089 - -0.9 343,286 - HMPREF1181_RS14090 0.43 -0.4 343,286 - HMPREF1181_RS14090 0.43 -0.8 343,346 + HMPREF1181_RS14090 0.56 -3.7 343,353 + HMPREF1181_RS14090 0.57 -3.1 343,353 + HMPREF1181_RS14090 0.57 -1.2 343,353 + HMPREF1181_RS14090 0.57 -5.4 343,354 - HMPREF1181_RS14090 0.57 -0.4 343,354 - HMPREF1181_RS14090 0.57 -0.3 343,367 - HMPREF1181_RS14090 0.60 +0.2 343,454 - HMPREF1181_RS14090 0.78 +0.6 343,457 + HMPREF1181_RS14090 0.78 -2.8 343,596 + -2.1 343,668 - -0.5 343,761 - HMPREF1181_RS14095 0.11 -0.2 343,925 + HMPREF1181_RS14095 0.36 +0.7 343,963 + HMPREF1181_RS14095 0.42 +0.3 344,035 + HMPREF1181_RS14095 0.53 +0.0 344,036 - HMPREF1181_RS14095 0.53 -1.6 344,042 - HMPREF1181_RS14095 0.54 -1.8 344,081 + HMPREF1181_RS14095 0.60 -1.7 344,613 + -0.0 344,698 + -0.4 344,736 - -0.6 344,759 + HMPREF1181_RS14100 0.10 -1.2 344,802 + HMPREF1181_RS14100 0.12 +0.4 344,822 - HMPREF1181_RS14100 0.13 +0.5 344,980 + HMPREF1181_RS14100 0.21 -1.5 344,981 - HMPREF1181_RS14100 0.21 -0.1 345,048 + HMPREF1181_RS14100 0.24 +0.7 345,052 + HMPREF1181_RS14100 0.24 +0.3 345,055 - HMPREF1181_RS14100 0.25 +1.3 345,055 - HMPREF1181_RS14100 0.25 -0.0 345,123 + HMPREF1181_RS14100 0.28 -1.9 345,172 - HMPREF1181_RS14100 0.30 -0.0 345,273 + HMPREF1181_RS14100 0.35 -0.1 345,306 + HMPREF1181_RS14100 0.37 -0.3 345,309 - HMPREF1181_RS14100 0.37 -0.4
Or see this region's nucleotide sequence