Experiment: Ying_Others16 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF246 and GFF247 are separated by 13 nucleotides GFF247 and GFF248 are separated by 16 nucleotides GFF248 and GFF249 are separated by 23 nucleotides GFF249 and GFF250 are separated by 26 nucleotides
GFF246 - Thiazole biosynthesis protein ThiG, at 263,378 to 264,196
GFF246
GFF247 - Sulfur carrier protein ThiS, at 264,210 to 264,407
GFF247
GFF248 - Glycine oxidase ThiO (EC 1.4.3.19), at 264,424 to 265,506
GFF248
GFF249 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 265,530 to 266,378
GFF249
GFF250 - Predicted hydroxymethylpyrimidine transporter CytX, at 266,405 to 267,712
GFF250
Position (kb) 264
265
266 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 264.533 kb on + strand, within GFF248 at 264.663 kb on - strand, within GFF248 at 264.773 kb on + strand, within GFF248 at 264.774 kb on - strand, within GFF248 at 265.049 kb on + strand, within GFF248 at 265.050 kb on - strand, within GFF248 at 265.560 kb on + strand at 265.560 kb on + strand at 265.560 kb on + strand at 265.560 kb on + strand at 265.560 kb on + strand at 265.560 kb on + strand at 265.843 kb on - strand, within GFF249 at 266.218 kb on - strand, within GFF249 at 266.268 kb on + strand, within GFF249 at 266.269 kb on - strand, within GFF249 at 266.269 kb on - strand, within GFF249 at 266.304 kb on + strand at 266.305 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 3 remove 264,533 + GFF248 0.10 -3.5 264,663 - GFF248 0.22 -1.7 264,773 + GFF248 0.32 -3.0 264,774 - GFF248 0.32 -2.7 265,049 + GFF248 0.58 -0.4 265,050 - GFF248 0.58 -1.9 265,560 + -1.7 265,560 + -3.1 265,560 + -3.1 265,560 + +0.2 265,560 + -3.0 265,560 + -2.2 265,843 - GFF249 0.37 -4.8 266,218 - GFF249 0.81 -4.7 266,268 + GFF249 0.87 -2.5 266,269 - GFF249 0.87 -1.4 266,269 - GFF249 0.87 -3.8 266,304 + -4.8 266,305 - -2.1
Or see this region's nucleotide sequence