Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF571 and GFF572 are separated by 17 nucleotides GFF572 and GFF573 are separated by 10 nucleotides GFF573 and GFF574 are separated by 4 nucleotides GFF574 and GFF575 overlap by 4 nucleotides
GFF571 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 242,619 to 244,172
GFF571
GFF572 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 244,190 to 244,738
GFF572
GFF573 - Phosphatidate cytidylyltransferase (EC 2.7.7.41), at 244,749 to 245,741
GFF573
GFF574 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 245,746 to 246,363
GFF574
GFF575 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 246,360 to 247,082
GFF575
Position (kb)
244
245
246 Strain fitness (log2 ratio)
-2
-1
0
1 at 243.883 kb on + strand, within GFF571 at 243.883 kb on + strand, within GFF571 at 243.884 kb on - strand, within GFF571 at 243.884 kb on - strand, within GFF571 at 244.194 kb on + strand at 244.194 kb on + strand at 244.194 kb on + strand at 244.195 kb on - strand at 244.212 kb on + strand at 244.428 kb on + strand, within GFF572 at 244.429 kb on - strand, within GFF572 at 244.659 kb on + strand, within GFF572 at 244.659 kb on + strand, within GFF572 at 244.659 kb on + strand, within GFF572 at 245.215 kb on + strand, within GFF573 at 245.215 kb on + strand, within GFF573 at 245.318 kb on - strand, within GFF573 at 245.318 kb on - strand, within GFF573 at 245.318 kb on - strand, within GFF573 at 245.371 kb on + strand, within GFF573 at 245.372 kb on - strand, within GFF573 at 245.954 kb on + strand, within GFF574 at 245.954 kb on + strand, within GFF574 at 245.954 kb on + strand, within GFF574 at 245.954 kb on + strand, within GFF574 at 245.955 kb on - strand, within GFF574 at 246.096 kb on - strand, within GFF574 at 246.096 kb on - strand, within GFF574 at 246.152 kb on + strand, within GFF574 at 246.152 kb on + strand, within GFF574 at 246.153 kb on - strand, within GFF574 at 246.251 kb on + strand, within GFF574 at 246.251 kb on + strand, within GFF574 at 246.251 kb on + strand, within GFF574 at 246.252 kb on - strand, within GFF574 at 246.252 kb on - strand, within GFF574 at 246.252 kb on - strand, within GFF574 at 246.252 kb on - strand, within GFF574 at 246.285 kb on - strand, within GFF574 at 246.419 kb on - strand at 246.419 kb on - strand at 246.419 kb on - strand at 246.419 kb on - strand at 246.521 kb on - strand, within GFF575 at 246.580 kb on + strand, within GFF575 at 246.580 kb on + strand, within GFF575 at 246.581 kb on - strand, within GFF575 at 246.581 kb on - strand, within GFF575
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 243,883 + GFF571 0.81 +0.3 243,883 + GFF571 0.81 -0.9 243,884 - GFF571 0.81 -0.2 243,884 - GFF571 0.81 +0.1 244,194 + -0.1 244,194 + -1.7 244,194 + -0.5 244,195 - -1.6 244,212 + -2.2 244,428 + GFF572 0.43 -0.1 244,429 - GFF572 0.44 -1.1 244,659 + GFF572 0.85 -1.4 244,659 + GFF572 0.85 -1.1 244,659 + GFF572 0.85 -0.7 245,215 + GFF573 0.47 -0.7 245,215 + GFF573 0.47 +0.9 245,318 - GFF573 0.57 +0.2 245,318 - GFF573 0.57 -0.2 245,318 - GFF573 0.57 +1.1 245,371 + GFF573 0.63 +1.7 245,372 - GFF573 0.63 -1.1 245,954 + GFF574 0.34 +0.6 245,954 + GFF574 0.34 +0.2 245,954 + GFF574 0.34 +0.6 245,954 + GFF574 0.34 -0.1 245,955 - GFF574 0.34 -0.2 246,096 - GFF574 0.57 +1.3 246,096 - GFF574 0.57 -0.1 246,152 + GFF574 0.66 +0.2 246,152 + GFF574 0.66 +0.5 246,153 - GFF574 0.66 -1.0 246,251 + GFF574 0.82 +0.4 246,251 + GFF574 0.82 -0.3 246,251 + GFF574 0.82 -1.4 246,252 - GFF574 0.82 -0.1 246,252 - GFF574 0.82 -1.4 246,252 - GFF574 0.82 -0.2 246,252 - GFF574 0.82 +0.6 246,285 - GFF574 0.87 -0.5 246,419 - +0.1 246,419 - +1.6 246,419 - -0.9 246,419 - +0.4 246,521 - GFF575 0.22 -0.9 246,580 + GFF575 0.30 -0.1 246,580 + GFF575 0.30 +0.2 246,581 - GFF575 0.31 +0.6 246,581 - GFF575 0.31 -0.9
Or see this region's nucleotide sequence