Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF83

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF81 and GFF82 are separated by 18 nucleotidesGFF82 and GFF83 are separated by 32 nucleotidesGFF83 and GFF84 overlap by 14 nucleotidesGFF84 and GFF85 overlap by 1 nucleotides GFF81 - Biopolymer transport protein ExbD/TolR, at 91,246 to 91,656 GFF81 GFF82 - Ferric siderophore transport system, periplasmic binding protein TonB, at 91,675 to 92,337 GFF82 GFF83 - Outer membrane receptor for ferric coprogen and ferric-rhodotorulic acid, at 92,370 to 93,929 GFF83 GFF84 - FOG: Ankyrin repeat, at 93,916 to 94,773 GFF84 GFF85 - Uncharacterized archaeal coiled-coil protein, at 94,773 to 95,426 GFF85 Position (kb) 92 93 94Strain fitness (log2 ratio) -1 0 1at 91.750 kb on + strand, within GFF82at 93.346 kb on - strand, within GFF83at 93.346 kb on - strand, within GFF83at 93.528 kb on + strand, within GFF83at 93.570 kb on + strand, within GFF83at 93.910 kb on - strandat 93.910 kb on - strandat 94.652 kb on - strand, within GFF84

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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91,750 + GFF82 0.11 -0.2
93,346 - GFF83 0.63 -0.7
93,346 - GFF83 0.63 -0.1
93,528 + GFF83 0.74 -0.8
93,570 + GFF83 0.77 -1.2
93,910 - -0.0
93,910 - +0.3
94,652 - GFF84 0.86 -0.3

Or see this region's nucleotide sequence