Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF515

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF514 and GFF515 are separated by 3 nucleotidesGFF515 and GFF516 are separated by 7 nucleotidesGFF516 and GFF517 are separated by 38 nucleotides GFF514 - Esterase/lipase, at 181,816 to 182,778 GFF514 GFF515 - Glutamine amidotransferase class-I (EC 6.3.5.2), at 182,782 to 183,522 GFF515 GFF516 - Acyl-CoA hydrolase (EC 3.1.2.20), at 183,530 to 183,970 GFF516 GFF517 - Lipid A export ATP-binding/permease protein MsbA, at 184,009 to 185,856 GFF517 Position (kb) 182 183 184Strain fitness (log2 ratio) -1 0 1 2at 181.924 kb on + strand, within GFF514at 181.976 kb on - strand, within GFF514at 181.976 kb on - strand, within GFF514at 181.976 kb on - strand, within GFF514at 182.014 kb on + strand, within GFF514at 182.014 kb on + strand, within GFF514at 182.059 kb on + strand, within GFF514at 182.059 kb on + strand, within GFF514at 182.059 kb on + strand, within GFF514at 182.059 kb on + strand, within GFF514at 182.059 kb on + strand, within GFF514at 182.059 kb on + strand, within GFF514at 182.060 kb on - strand, within GFF514at 182.060 kb on - strand, within GFF514at 182.060 kb on - strand, within GFF514at 182.060 kb on - strand, within GFF514at 182.060 kb on - strand, within GFF514at 182.140 kb on + strand, within GFF514at 182.140 kb on + strand, within GFF514at 182.141 kb on - strand, within GFF514at 182.141 kb on - strand, within GFF514at 182.562 kb on + strand, within GFF514at 182.639 kb on - strand, within GFF514at 182.651 kb on + strand, within GFF514at 182.652 kb on - strand, within GFF514at 182.652 kb on - strand, within GFF514at 182.921 kb on - strand, within GFF515at 183.205 kb on + strand, within GFF515at 183.205 kb on + strand, within GFF515at 183.206 kb on - strand, within GFF515at 183.380 kb on - strand, within GFF515at 183.380 kb on - strand, within GFF515at 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.607 kb on - strand, within GFF516at 183.607 kb on - strand, within GFF516at 183.607 kb on - strand, within GFF516at 183.636 kb on + strand, within GFF516at 183.636 kb on + strand, within GFF516at 183.804 kb on + strand, within GFF516at 183.804 kb on + strand, within GFF516at 184.124 kb on - strandat 184.135 kb on + strandat 184.136 kb on - strandat 184.504 kb on + strand, within GFF517at 184.505 kb on - strand, within GFF517at 184.514 kb on - strand, within GFF517

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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181,924 + GFF514 0.11 +0.6
181,976 - GFF514 0.17 -0.8
181,976 - GFF514 0.17 -0.1
181,976 - GFF514 0.17 -0.2
182,014 + GFF514 0.21 -0.4
182,014 + GFF514 0.21 +0.4
182,059 + GFF514 0.25 -1.5
182,059 + GFF514 0.25 -0.1
182,059 + GFF514 0.25 -0.7
182,059 + GFF514 0.25 +0.1
182,059 + GFF514 0.25 -0.2
182,059 + GFF514 0.25 +1.7
182,060 - GFF514 0.25 -0.0
182,060 - GFF514 0.25 +0.1
182,060 - GFF514 0.25 +0.4
182,060 - GFF514 0.25 -1.7
182,060 - GFF514 0.25 +0.2
182,140 + GFF514 0.34 -0.1
182,140 + GFF514 0.34 +0.4
182,141 - GFF514 0.34 -0.6
182,141 - GFF514 0.34 +0.2
182,562 + GFF514 0.77 +0.0
182,639 - GFF514 0.85 +0.3
182,651 + GFF514 0.87 +0.8
182,652 - GFF514 0.87 +0.2
182,652 - GFF514 0.87 +0.1
182,921 - GFF515 0.19 +0.4
183,205 + GFF515 0.57 -0.0
183,205 + GFF515 0.57 -0.2
183,206 - GFF515 0.57 +0.5
183,380 - GFF515 0.81 +0.2
183,380 - GFF515 0.81 +0.6
183,487 + +0.6
183,487 + +0.6
183,487 + -0.2
183,487 + -0.3
183,487 + -0.1
183,607 - GFF516 0.17 -0.5
183,607 - GFF516 0.17 -0.5
183,607 - GFF516 0.17 +0.7
183,636 + GFF516 0.24 +2.0
183,636 + GFF516 0.24 -0.0
183,804 + GFF516 0.62 +0.7
183,804 + GFF516 0.62 +0.2
184,124 - +0.5
184,135 + -0.2
184,136 - +0.2
184,504 + GFF517 0.27 -0.2
184,505 - GFF517 0.27 +0.1
184,514 - GFF517 0.27 -0.9

Or see this region's nucleotide sequence