Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3079 and GFF3080 are separated by 95 nucleotides GFF3080 and GFF3081 are separated by 11 nucleotides GFF3081 and GFF3082 overlap by 14 nucleotides
GFF3079 - Transcriptional regulator, TetR family, at 156,360 to 156,932
GFF3079
GFF3080 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 157,028 to 157,489
GFF3080
GFF3081 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 157,501 to 159,765
GFF3081
GFF3082 - Hydrogen peroxide-inducible genes activator, at 159,752 to 160,735
GFF3082
Position (kb)
157
158
159
160 Strain fitness (log2 ratio)
-1
0
1 at 156.534 kb on + strand, within GFF3079 at 156.534 kb on + strand, within GFF3079 at 156.535 kb on - strand, within GFF3079 at 156.535 kb on - strand, within GFF3079 at 156.681 kb on + strand, within GFF3079 at 156.681 kb on + strand, within GFF3079 at 156.681 kb on + strand, within GFF3079 at 156.681 kb on + strand, within GFF3079 at 156.682 kb on - strand, within GFF3079 at 156.682 kb on - strand, within GFF3079 at 156.684 kb on + strand, within GFF3079 at 156.684 kb on + strand, within GFF3079 at 156.684 kb on + strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.685 kb on - strand, within GFF3079 at 156.969 kb on - strand at 156.995 kb on + strand at 156.996 kb on - strand at 156.996 kb on - strand at 157.002 kb on - strand at 157.002 kb on - strand at 157.439 kb on + strand, within GFF3080 at 157.439 kb on + strand, within GFF3080 at 157.440 kb on - strand, within GFF3080 at 157.440 kb on - strand, within GFF3080 at 157.440 kb on - strand, within GFF3080 at 157.652 kb on - strand at 157.885 kb on + strand, within GFF3081 at 157.885 kb on + strand, within GFF3081 at 157.886 kb on - strand, within GFF3081 at 157.886 kb on - strand, within GFF3081 at 157.945 kb on + strand, within GFF3081 at 157.945 kb on + strand, within GFF3081 at 157.945 kb on + strand, within GFF3081 at 157.945 kb on + strand, within GFF3081 at 157.945 kb on + strand, within GFF3081 at 157.945 kb on + strand, within GFF3081 at 157.945 kb on + strand, within GFF3081 at 157.946 kb on - strand, within GFF3081 at 157.946 kb on - strand, within GFF3081 at 157.946 kb on - strand, within GFF3081 at 158.494 kb on + strand, within GFF3081 at 158.494 kb on + strand, within GFF3081 at 158.495 kb on - strand, within GFF3081 at 158.596 kb on + strand, within GFF3081 at 158.597 kb on - strand, within GFF3081 at 158.917 kb on + strand, within GFF3081 at 158.917 kb on + strand, within GFF3081 at 159.179 kb on - strand, within GFF3081 at 159.179 kb on - strand, within GFF3081 at 159.179 kb on - strand, within GFF3081 at 159.238 kb on + strand, within GFF3081 at 159.239 kb on - strand, within GFF3081 at 159.239 kb on - strand, within GFF3081 at 159.388 kb on + strand, within GFF3081 at 159.388 kb on + strand, within GFF3081 at 159.388 kb on + strand, within GFF3081 at 159.388 kb on + strand, within GFF3081 at 159.388 kb on + strand, within GFF3081 at 159.388 kb on + strand, within GFF3081 at 159.389 kb on - strand, within GFF3081 at 159.389 kb on - strand, within GFF3081 at 159.924 kb on + strand, within GFF3082 at 159.924 kb on + strand, within GFF3082 at 159.924 kb on + strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 159.925 kb on - strand, within GFF3082 at 160.017 kb on + strand, within GFF3082 at 160.101 kb on + strand, within GFF3082 at 160.699 kb on + strand at 160.699 kb on + strand at 160.700 kb on - strand at 160.700 kb on - strand at 160.761 kb on + strand at 160.761 kb on + strand at 160.761 kb on + strand at 160.761 kb on + strand at 160.762 kb on - strand at 160.762 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 156,534 + GFF3079 0.30 -0.8 156,534 + GFF3079 0.30 -0.0 156,535 - GFF3079 0.31 +0.7 156,535 - GFF3079 0.31 -0.1 156,681 + GFF3079 0.56 +0.3 156,681 + GFF3079 0.56 -0.2 156,681 + GFF3079 0.56 -0.7 156,681 + GFF3079 0.56 -1.1 156,682 - GFF3079 0.56 -0.1 156,682 - GFF3079 0.56 -0.4 156,684 + GFF3079 0.57 -0.9 156,684 + GFF3079 0.57 -1.5 156,684 + GFF3079 0.57 -0.3 156,685 - GFF3079 0.57 +0.1 156,685 - GFF3079 0.57 +0.5 156,685 - GFF3079 0.57 -1.4 156,685 - GFF3079 0.57 -0.2 156,685 - GFF3079 0.57 -1.3 156,969 - -0.4 156,995 + +0.2 156,996 - +0.3 156,996 - +0.2 157,002 - -0.7 157,002 - +0.6 157,439 + GFF3080 0.89 -0.7 157,439 + GFF3080 0.89 +0.6 157,440 - GFF3080 0.89 -0.3 157,440 - GFF3080 0.89 +0.1 157,440 - GFF3080 0.89 +0.0 157,652 - +0.3 157,885 + GFF3081 0.17 -1.4 157,885 + GFF3081 0.17 +0.5 157,886 - GFF3081 0.17 -0.5 157,886 - GFF3081 0.17 +0.6 157,945 + GFF3081 0.20 -1.3 157,945 + GFF3081 0.20 -0.1 157,945 + GFF3081 0.20 +1.1 157,945 + GFF3081 0.20 +0.3 157,945 + GFF3081 0.20 +0.2 157,945 + GFF3081 0.20 +0.2 157,945 + GFF3081 0.20 +0.8 157,946 - GFF3081 0.20 -0.4 157,946 - GFF3081 0.20 +0.6 157,946 - GFF3081 0.20 -0.1 158,494 + GFF3081 0.44 +0.2 158,494 + GFF3081 0.44 +0.4 158,495 - GFF3081 0.44 +1.5 158,596 + GFF3081 0.48 -0.1 158,597 - GFF3081 0.48 +0.2 158,917 + GFF3081 0.63 -0.9 158,917 + GFF3081 0.63 +0.1 159,179 - GFF3081 0.74 -0.0 159,179 - GFF3081 0.74 +0.1 159,179 - GFF3081 0.74 +0.5 159,238 + GFF3081 0.77 +0.3 159,239 - GFF3081 0.77 +1.1 159,239 - GFF3081 0.77 +1.6 159,388 + GFF3081 0.83 -0.2 159,388 + GFF3081 0.83 +0.1 159,388 + GFF3081 0.83 -0.4 159,388 + GFF3081 0.83 -0.0 159,388 + GFF3081 0.83 +1.1 159,388 + GFF3081 0.83 -0.5 159,389 - GFF3081 0.83 -0.4 159,389 - GFF3081 0.83 +0.5 159,924 + GFF3082 0.17 +0.1 159,924 + GFF3082 0.17 -0.7 159,924 + GFF3082 0.17 +0.3 159,925 - GFF3082 0.18 +0.1 159,925 - GFF3082 0.18 -0.1 159,925 - GFF3082 0.18 -0.1 159,925 - GFF3082 0.18 -0.4 159,925 - GFF3082 0.18 -0.2 159,925 - GFF3082 0.18 +0.7 159,925 - GFF3082 0.18 +1.2 159,925 - GFF3082 0.18 +0.1 159,925 - GFF3082 0.18 +0.7 160,017 + GFF3082 0.27 +0.8 160,101 + GFF3082 0.35 -0.4 160,699 + -0.2 160,699 + -0.3 160,700 - +1.1 160,700 - -0.0 160,761 + -0.2 160,761 + +0.1 160,761 + +0.1 160,761 + -0.9 160,762 - -0.5 160,762 - +0.9
Or see this region's nucleotide sequence