Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1821 and GFF1822 are separated by 4 nucleotides GFF1822 and GFF1823 are separated by 12 nucleotides GFF1823 and GFF1824 are separated by 15 nucleotides
GFF1821 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 686,829 to 687,836
GFF1821
GFF1822 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 687,841 to 688,635
GFF1822
GFF1823 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 688,648 to 689,466
GFF1823
GFF1824 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 689,482 to 690,309
GFF1824
Position (kb) 687
688
689 Strain fitness (log2 ratio) -2
-1
0
1
2 at 686.845 kb on + strand at 687.209 kb on - strand, within GFF1821 at 687.209 kb on - strand, within GFF1821 at 687.852 kb on - strand at 687.852 kb on - strand at 688.187 kb on + strand, within GFF1822 at 688.187 kb on + strand, within GFF1822 at 688.188 kb on - strand, within GFF1822 at 688.188 kb on - strand, within GFF1822 at 688.217 kb on + strand, within GFF1822 at 688.218 kb on - strand, within GFF1822 at 688.427 kb on + strand, within GFF1822 at 688.427 kb on + strand, within GFF1822 at 688.427 kb on + strand, within GFF1822 at 688.428 kb on - strand, within GFF1822 at 688.428 kb on - strand, within GFF1822 at 688.428 kb on - strand, within GFF1822 at 688.508 kb on + strand, within GFF1822 at 688.508 kb on + strand, within GFF1822 at 688.508 kb on + strand, within GFF1822 at 688.508 kb on + strand, within GFF1822 at 688.508 kb on + strand, within GFF1822 at 688.508 kb on + strand, within GFF1822 at 688.509 kb on - strand, within GFF1822 at 688.509 kb on - strand, within GFF1822 at 688.509 kb on - strand, within GFF1822 at 688.509 kb on - strand, within GFF1822 at 688.548 kb on - strand, within GFF1822 at 688.649 kb on + strand at 688.649 kb on + strand at 688.650 kb on - strand at 688.650 kb on - strand at 688.652 kb on + strand at 688.652 kb on + strand at 688.652 kb on + strand at 688.653 kb on - strand at 688.653 kb on - strand at 688.653 kb on - strand at 688.653 kb on - strand at 688.653 kb on - strand at 688.785 kb on - strand, within GFF1823 at 689.049 kb on - strand, within GFF1823 at 689.381 kb on + strand, within GFF1823 at 689.381 kb on + strand, within GFF1823 at 689.381 kb on + strand, within GFF1823 at 689.382 kb on - strand, within GFF1823 at 689.382 kb on - strand, within GFF1823
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 686,845 + +0.0 687,209 - GFF1821 0.38 +1.4 687,209 - GFF1821 0.38 -0.2 687,852 - +0.2 687,852 - -0.9 688,187 + GFF1822 0.44 +0.3 688,187 + GFF1822 0.44 +0.1 688,188 - GFF1822 0.44 +1.7 688,188 - GFF1822 0.44 +0.3 688,217 + GFF1822 0.47 +0.3 688,218 - GFF1822 0.47 -1.2 688,427 + GFF1822 0.74 +0.6 688,427 + GFF1822 0.74 -0.5 688,427 + GFF1822 0.74 -1.2 688,428 - GFF1822 0.74 +0.3 688,428 - GFF1822 0.74 -0.2 688,428 - GFF1822 0.74 +1.1 688,508 + GFF1822 0.84 -1.9 688,508 + GFF1822 0.84 -0.4 688,508 + GFF1822 0.84 -0.4 688,508 + GFF1822 0.84 +0.4 688,508 + GFF1822 0.84 +0.4 688,508 + GFF1822 0.84 -0.4 688,509 - GFF1822 0.84 -0.2 688,509 - GFF1822 0.84 -0.3 688,509 - GFF1822 0.84 -0.1 688,509 - GFF1822 0.84 +0.3 688,548 - GFF1822 0.89 -0.2 688,649 + -0.4 688,649 + +1.1 688,650 - -0.2 688,650 - -0.3 688,652 + -0.3 688,652 + +0.1 688,652 + -0.2 688,653 - -0.6 688,653 - +0.4 688,653 - +0.1 688,653 - +1.9 688,653 - +0.8 688,785 - GFF1823 0.17 +1.1 689,049 - GFF1823 0.49 +0.4 689,381 + GFF1823 0.89 +1.1 689,381 + GFF1823 0.89 -0.1 689,381 + GFF1823 0.89 -1.2 689,382 - GFF1823 0.90 -1.0 689,382 - GFF1823 0.90 +0.5
Or see this region's nucleotide sequence