Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF175 and GFF176 are separated by 8 nucleotides GFF176 and GFF177 are separated by 24 nucleotides GFF177 and GFF178 overlap by 14 nucleotides
GFF175 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 189,129 to 190,142
GFF175
GFF176 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 190,151 to 191,107
GFF176
GFF177 - Demethylmenaquinone methyltransferase, at 191,132 to 191,791
GFF177
GFF178 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 191,778 to 192,737
GFF178
Position (kb) 190
191
192 Strain fitness (log2 ratio) -1
0
1 at 189.225 kb on + strand at 189.334 kb on - strand, within GFF175 at 189.334 kb on - strand, within GFF175 at 189.436 kb on - strand, within GFF175 at 189.515 kb on - strand, within GFF175 at 189.843 kb on + strand, within GFF175 at 189.858 kb on + strand, within GFF175 at 189.858 kb on + strand, within GFF175 at 189.859 kb on - strand, within GFF175 at 190.884 kb on - strand, within GFF176 at 191.006 kb on + strand, within GFF176 at 191.526 kb on - strand, within GFF177 at 191.526 kb on - strand, within GFF177 at 191.778 kb on - strand at 191.884 kb on - strand, within GFF178 at 191.884 kb on - strand, within GFF178 at 191.956 kb on - strand, within GFF178
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 189,225 + +0.0 189,334 - GFF175 0.20 -1.1 189,334 - GFF175 0.20 +1.0 189,436 - GFF175 0.30 +0.1 189,515 - GFF175 0.38 +0.0 189,843 + GFF175 0.70 +0.0 189,858 + GFF175 0.72 -0.8 189,858 + GFF175 0.72 +1.6 189,859 - GFF175 0.72 -0.4 190,884 - GFF176 0.77 +0.1 191,006 + GFF176 0.89 +0.4 191,526 - GFF177 0.60 -0.5 191,526 - GFF177 0.60 -0.1 191,778 - +0.2 191,884 - GFF178 0.11 +0.4 191,884 - GFF178 0.11 +0.0 191,956 - GFF178 0.19 +1.0
Or see this region's nucleotide sequence