Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF143 and GFF144 are separated by 97 nucleotides GFF144 and GFF145 are separated by 4 nucleotides GFF145 and GFF146 overlap by 1 nucleotides GFF146 and GFF147 are separated by 1 nucleotides
GFF143 - D-glycerate 2-kinase (EC 2.7.1.-), at 158,761 to 160,086
GFF143
GFF144 - hypothetical protein, at 160,184 to 160,816
GFF144
GFF145 - Methyl-accepting chemotaxis protein, at 160,821 to 163,067
GFF145
GFF146 - Flagellar motor protein, at 163,067 to 163,729
GFF146
GFF147 - FIG01210206: hypothetical protein, at 163,731 to 164,264
GFF147
Position (kb)
160
161
162
163
164 Strain fitness (log2 ratio)
-1
0
1
2 at 159.997 kb on + strand at 159.998 kb on - strand at 160.173 kb on - strand at 160.959 kb on + strand at 160.959 kb on + strand at 160.960 kb on - strand at 161.616 kb on + strand, within GFF145 at 161.617 kb on - strand, within GFF145 at 161.823 kb on + strand, within GFF145 at 161.823 kb on + strand, within GFF145 at 162.952 kb on - strand at 162.952 kb on - strand at 162.954 kb on + strand at 162.955 kb on - strand at 162.955 kb on - strand at 163.436 kb on + strand, within GFF146 at 163.436 kb on + strand, within GFF146 at 163.436 kb on + strand, within GFF146 at 163.764 kb on + strand at 163.764 kb on + strand at 163.765 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 159,997 + -0.6 159,998 - -0.2 160,173 - +0.1 160,959 + +2.0 160,959 + -0.2 160,960 - -0.2 161,616 + GFF145 0.35 -0.2 161,617 - GFF145 0.35 -0.5 161,823 + GFF145 0.45 +0.1 161,823 + GFF145 0.45 +0.3 162,952 - +0.1 162,952 - +0.0 162,954 + +1.0 162,955 - +0.3 162,955 - +0.3 163,436 + GFF146 0.56 -1.6 163,436 + GFF146 0.56 -0.5 163,436 + GFF146 0.56 -0.3 163,764 + -1.0 163,764 + -1.0 163,765 - +0.5
Or see this region's nucleotide sequence