Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF1155

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1153 and GFF1154 are separated by 75 nucleotidesGFF1154 and GFF1155 overlap by 4 nucleotidesGFF1155 and GFF1156 overlap by 4 nucleotidesGFF1156 and GFF1157 are separated by 30 nucleotides GFF1153 - Transcriptional regulator, LysR family, at 9,171 to 10,127 GFF1153 GFF1154 - Enoyl-CoA hydratase (EC 4.2.1.17), at 10,203 to 11,114 GFF1154 GFF1155 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 11,111 to 13,276 GFF1155 GFF1156 - 2-hydroxychromene-2-carboxylate isomerase, at 13,273 to 13,881 GFF1156 GFF1157 - Tricarboxylate transport protein TctC, at 13,912 to 14,916 GFF1157 Position (kb) 11 12 13 14Strain fitness (log2 ratio) -1 0 1at 11.804 kb on + strand, within GFF1155at 11.984 kb on + strand, within GFF1155at 12.459 kb on - strand, within GFF1155at 12.708 kb on - strand, within GFF1155at 13.324 kb on + strandat 13.324 kb on + strandat 13.325 kb on - strandat 14.272 kb on + strand, within GFF1157at 14.273 kb on - strand, within GFF1157

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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11,804 + GFF1155 0.32 +0.7
11,984 + GFF1155 0.40 +0.2
12,459 - GFF1155 0.62 -0.3
12,708 - GFF1155 0.74 +0.3
13,324 + -0.6
13,324 + +0.0
13,325 - +0.4
14,272 + GFF1157 0.36 +0.5
14,273 - GFF1157 0.36 +0.1

Or see this region's nucleotide sequence