Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF648 and GFF649 overlap by 4 nucleotides GFF649 and GFF650 overlap by 4 nucleotides GFF650 and GFF651 are separated by 2 nucleotides GFF651 and GFF652 are separated by 4 nucleotides
GFF648 - UPF0301 protein YqgE, at 330,241 to 330,828
GFF648
GFF649 - Putative Holliday junction resolvase YggF, at 330,825 to 331,253
GFF649
GFF650 - Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR, at 331,250 to 331,774
GFF650
GFF651 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 331,777 to 332,736
GFF651
GFF652 - Dihydroorotase (EC 3.5.2.3), at 332,741 to 334,066
GFF652
Position (kb) 331
332 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 330.541 kb on + strand, within GFF648 at 330.541 kb on + strand, within GFF648 at 330.541 kb on + strand, within GFF648 at 330.541 kb on + strand, within GFF648 at 330.542 kb on - strand, within GFF648 at 330.542 kb on - strand, within GFF648 at 330.542 kb on - strand, within GFF648 at 330.542 kb on - strand, within GFF648 at 330.542 kb on - strand, within GFF648 at 330.652 kb on + strand, within GFF648 at 330.653 kb on - strand, within GFF648 at 330.653 kb on - strand, within GFF648 at 331.550 kb on + strand, within GFF650 at 331.550 kb on + strand, within GFF650 at 331.550 kb on + strand, within GFF650 at 331.551 kb on - strand, within GFF650 at 331.595 kb on + strand, within GFF650 at 332.150 kb on - strand, within GFF651 at 332.242 kb on + strand, within GFF651 at 332.242 kb on + strand, within GFF651 at 332.242 kb on + strand, within GFF651 at 332.242 kb on + strand, within GFF651 at 332.242 kb on + strand, within GFF651 at 332.242 kb on + strand, within GFF651 at 332.242 kb on + strand, within GFF651 at 332.243 kb on - strand, within GFF651 at 332.243 kb on - strand, within GFF651 at 332.258 kb on - strand, within GFF651 at 332.530 kb on + strand, within GFF651 at 332.530 kb on + strand, within GFF651 at 332.530 kb on + strand, within GFF651 at 332.531 kb on - strand, within GFF651 at 332.680 kb on + strand at 332.680 kb on + strand at 332.681 kb on - strand at 332.734 kb on + strand at 332.734 kb on + strand at 332.735 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 330,541 + GFF648 0.51 -0.5 330,541 + GFF648 0.51 +0.7 330,541 + GFF648 0.51 -0.3 330,541 + GFF648 0.51 -0.8 330,542 - GFF648 0.51 +0.1 330,542 - GFF648 0.51 +0.8 330,542 - GFF648 0.51 +1.6 330,542 - GFF648 0.51 -0.4 330,542 - GFF648 0.51 +0.2 330,652 + GFF648 0.70 -1.0 330,653 - GFF648 0.70 +0.0 330,653 - GFF648 0.70 +0.0 331,550 + GFF650 0.57 -0.7 331,550 + GFF650 0.57 +1.5 331,550 + GFF650 0.57 -1.4 331,551 - GFF650 0.57 -1.1 331,595 + GFF650 0.66 -0.4 332,150 - GFF651 0.39 -2.8 332,242 + GFF651 0.48 -0.6 332,242 + GFF651 0.48 -1.8 332,242 + GFF651 0.48 -2.3 332,242 + GFF651 0.48 -2.4 332,242 + GFF651 0.48 +0.0 332,242 + GFF651 0.48 -3.9 332,242 + GFF651 0.48 -3.4 332,243 - GFF651 0.49 -4.2 332,243 - GFF651 0.49 -3.7 332,258 - GFF651 0.50 -1.0 332,530 + GFF651 0.78 -3.2 332,530 + GFF651 0.78 -2.2 332,530 + GFF651 0.78 -3.4 332,531 - GFF651 0.79 -1.3 332,680 + -1.8 332,680 + -2.0 332,681 - -1.3 332,734 + -1.6 332,734 + -0.9 332,735 - +0.3
Or see this region's nucleotide sequence