Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3300

Experiment: Ying_AminoAcid20 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3298 and GFF3299 overlap by 15 nucleotidesGFF3299 and GFF3300 are separated by 41 nucleotidesGFF3300 and GFF3301 are separated by 382 nucleotidesGFF3301 and GFF3302 are separated by 11 nucleotides GFF3298 - Hydroxymethylpyrimidine ABC transporter, substrate-binding component, at 182,804 to 183,838 GFF3298 GFF3299 - hypothetical protein, at 183,824 to 184,078 GFF3299 GFF3300 - Putative Rieske (2Fe-2S) family oxidoreductase, large subunit, at 184,120 to 185,139 GFF3300 GFF3301 - Cytosine deaminase (EC 3.5.4.1), at 185,522 to 185,983 GFF3301 GFF3302 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 185,995 to 186,762 GFF3302 Position (kb) 184 185 186Strain fitness (log2 ratio) -1 0 1at 183.264 kb on + strand, within GFF3298at 183.495 kb on + strand, within GFF3298at 183.495 kb on + strand, within GFF3298at 183.496 kb on - strand, within GFF3298at 183.504 kb on + strand, within GFF3298at 183.504 kb on + strand, within GFF3298at 183.667 kb on - strand, within GFF3298at 183.667 kb on - strand, within GFF3298at 183.903 kb on + strand, within GFF3299at 183.904 kb on - strand, within GFF3299at 183.904 kb on - strand, within GFF3299at 183.904 kb on - strand, within GFF3299at 183.904 kb on - strand, within GFF3299at 183.956 kb on - strand, within GFF3299at 184.033 kb on - strand, within GFF3299at 184.099 kb on + strandat 184.436 kb on - strand, within GFF3300at 184.459 kb on + strand, within GFF3300at 184.666 kb on + strand, within GFF3300at 184.793 kb on - strand, within GFF3300at 184.795 kb on + strand, within GFF3300at 185.794 kb on - strand, within GFF3301at 185.805 kb on + strand, within GFF3301at 186.062 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3
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183,264 + GFF3298 0.44 -1.3
183,495 + GFF3298 0.67 -0.1
183,495 + GFF3298 0.67 +0.8
183,496 - GFF3298 0.67 +0.5
183,504 + GFF3298 0.68 -0.2
183,504 + GFF3298 0.68 -0.7
183,667 - GFF3298 0.83 +0.3
183,667 - GFF3298 0.83 -0.9
183,903 + GFF3299 0.31 -0.2
183,904 - GFF3299 0.31 +0.2
183,904 - GFF3299 0.31 -0.3
183,904 - GFF3299 0.31 -0.1
183,904 - GFF3299 0.31 -0.7
183,956 - GFF3299 0.52 +0.7
184,033 - GFF3299 0.82 -0.1
184,099 + -0.7
184,436 - GFF3300 0.31 +1.3
184,459 + GFF3300 0.33 +0.6
184,666 + GFF3300 0.54 +0.2
184,793 - GFF3300 0.66 +0.1
184,795 + GFF3300 0.66 -0.1
185,794 - GFF3301 0.59 +0.3
185,805 + GFF3301 0.61 +0.0
186,062 + +0.6

Or see this region's nucleotide sequence