Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3065 and GFF3066 are separated by 271 nucleotides GFF3066 and GFF3067 overlap by 4 nucleotides GFF3067 and GFF3068 are separated by 4 nucleotides
GFF3065 - Periplasmic thiol:disulfide interchange protein DsbA, at 145,213 to 145,863
GFF3065
GFF3066 - LptA, protein essential for LPS transport across the periplasm, at 146,135 to 146,608
GFF3066
GFF3067 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 146,605 to 147,402
GFF3067
GFF3068 - RNA polymerase sigma-54 factor RpoN, at 147,407 to 149,035
GFF3068
Position (kb) 146
147
148 Strain fitness (log2 ratio) -1
0
1 at 145.747 kb on + strand, within GFF3065 at 145.747 kb on + strand, within GFF3065 at 145.747 kb on + strand, within GFF3065 at 145.748 kb on - strand, within GFF3065 at 145.789 kb on + strand, within GFF3065 at 145.790 kb on - strand, within GFF3065 at 145.792 kb on + strand, within GFF3065 at 145.792 kb on + strand, within GFF3065 at 145.792 kb on + strand, within GFF3065 at 145.792 kb on + strand, within GFF3065 at 145.792 kb on + strand, within GFF3065 at 145.792 kb on + strand, within GFF3065 at 145.793 kb on - strand, within GFF3065 at 145.793 kb on - strand, within GFF3065 at 145.793 kb on - strand, within GFF3065 at 145.793 kb on - strand, within GFF3065 at 147.962 kb on + strand, within GFF3068 at 147.962 kb on + strand, within GFF3068 at 147.962 kb on + strand, within GFF3068 at 147.963 kb on - strand, within GFF3068 at 147.996 kb on - strand, within GFF3068 at 147.996 kb on - strand, within GFF3068 at 147.996 kb on - strand, within GFF3068
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 145,747 + GFF3065 0.82 +0.3 145,747 + GFF3065 0.82 +0.3 145,747 + GFF3065 0.82 -0.7 145,748 - GFF3065 0.82 +0.5 145,789 + GFF3065 0.88 +0.5 145,790 - GFF3065 0.89 +0.8 145,792 + GFF3065 0.89 +0.8 145,792 + GFF3065 0.89 +0.3 145,792 + GFF3065 0.89 +0.9 145,792 + GFF3065 0.89 +0.5 145,792 + GFF3065 0.89 +0.3 145,792 + GFF3065 0.89 +0.9 145,793 - GFF3065 0.89 +0.4 145,793 - GFF3065 0.89 -1.6 145,793 - GFF3065 0.89 -0.6 145,793 - GFF3065 0.89 +0.1 147,962 + GFF3068 0.34 -0.3 147,962 + GFF3068 0.34 -0.5 147,962 + GFF3068 0.34 -0.1 147,963 - GFF3068 0.34 -0.1 147,996 - GFF3068 0.36 -0.3 147,996 - GFF3068 0.36 -1.0 147,996 - GFF3068 0.36 +0.6
Or see this region's nucleotide sequence