Experiment: Ying_AminoAcid20 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1309 and GFF1310 are separated by 8 nucleotides GFF1310 and GFF1311 are separated by 27 nucleotides GFF1311 and GFF1312 are separated by 13 nucleotides GFF1312 and GFF1313 overlap by 4 nucleotides
GFF1309 - Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-), at 160,767 to 161,546
GFF1309
GFF1310 - Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16), at 161,555 to 162,295
GFF1310
GFF1311 - Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-), at 162,323 to 162,985
GFF1311
GFF1312 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 162,999 to 163,619
GFF1312
GFF1313 - Histidinol-phosphate aminotransferase (EC 2.6.1.9), at 163,616 to 164,704
GFF1313
Position (kb) 162
163 Strain fitness (log2 ratio) -1
0
1
2
3 at 163.620 kb on - strand at 163.620 kb on - strand at 163.620 kb on - strand at 163.620 kb on - strand at 163.666 kb on + strand at 163.667 kb on - strand at 163.667 kb on - strand at 163.667 kb on - strand at 163.667 kb on - strand at 163.711 kb on + strand at 163.711 kb on + strand at 163.711 kb on + strand at 163.711 kb on + strand at 163.712 kb on - strand at 163.712 kb on - strand at 163.712 kb on - strand at 163.712 kb on - strand at 163.950 kb on + strand, within GFF1313 at 163.950 kb on + strand, within GFF1313 at 163.951 kb on - strand, within GFF1313 at 163.951 kb on - strand, within GFF1313 at 163.951 kb on - strand, within GFF1313 at 163.951 kb on - strand, within GFF1313 at 163.951 kb on - strand, within GFF1313 at 163.951 kb on - strand, within GFF1313
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 3 remove 163,620 - +3.3 163,620 - -0.4 163,620 - -0.4 163,620 - -1.1 163,666 + -0.0 163,667 - -0.0 163,667 - +0.1 163,667 - +0.7 163,667 - -0.3 163,711 + +0.5 163,711 + +0.6 163,711 + +0.5 163,711 + -1.2 163,712 - -0.0 163,712 - -0.4 163,712 - -0.4 163,712 - -0.0 163,950 + GFF1313 0.31 -0.9 163,950 + GFF1313 0.31 -0.2 163,951 - GFF1313 0.31 +0.4 163,951 - GFF1313 0.31 -0.8 163,951 - GFF1313 0.31 +1.0 163,951 - GFF1313 0.31 -0.2 163,951 - GFF1313 0.31 +0.6 163,951 - GFF1313 0.31 +0.6
Or see this region's nucleotide sequence