Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF3147

Experiment: Ying_all64 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3145 and GFF3146 overlap by 4 nucleotidesGFF3146 and GFF3147 overlap by 14 nucleotidesGFF3147 and GFF3148 overlap by 4 nucleotidesGFF3148 and GFF3149 overlap by 14 nucleotides GFF3145 - Conjugative transfer protein TraV, at 23,883 to 24,620 GFF3145 GFF3146 - Thiol:disulfide involved in conjugative transfer, at 24,617 to 25,486 GFF3146 GFF3147 - IncF plasmid conjugative transfer pilus assembly protein TraB, at 25,473 to 26,891 GFF3147 GFF3148 - IncF plasmid conjugative transfer pilus assembly protein TraK, at 26,888 to 27,883 GFF3148 GFF3149 - IncF plasmid conjugative transfer pilus assembly protein TraE, at 27,870 to 28,436 GFF3149 Position (kb) 25 26 27Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 24.514 kb on - strand, within GFF3145at 24.564 kb on - strandat 24.842 kb on + strand, within GFF3146at 24.933 kb on + strand, within GFF3146at 25.079 kb on + strand, within GFF3146at 25.228 kb on - strand, within GFF3146at 25.228 kb on - strand, within GFF3146at 25.258 kb on - strand, within GFF3146at 25.258 kb on - strand, within GFF3146at 25.477 kb on + strandat 25.478 kb on - strandat 25.478 kb on - strandat 25.657 kb on + strand, within GFF3147at 25.658 kb on - strand, within GFF3147at 25.660 kb on + strand, within GFF3147at 25.661 kb on - strand, within GFF3147at 25.661 kb on - strand, within GFF3147at 25.768 kb on + strand, within GFF3147at 26.916 kb on + strandat 26.916 kb on + strandat 26.917 kb on - strandat 26.917 kb on - strandat 26.917 kb on - strandat 27.337 kb on - strand, within GFF3148at 27.337 kb on - strand, within GFF3148at 27.402 kb on + strand, within GFF3148at 27.402 kb on + strand, within GFF3148at 27.403 kb on - strand, within GFF3148at 27.403 kb on - strand, within GFF3148

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 3
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24,514 - GFF3145 0.86 -0.1
24,564 - +0.9
24,842 + GFF3146 0.26 +4.1
24,933 + GFF3146 0.36 -1.9
25,079 + GFF3146 0.53 -1.4
25,228 - GFF3146 0.70 +0.7
25,228 - GFF3146 0.70 +1.3
25,258 - GFF3146 0.74 +0.2
25,258 - GFF3146 0.74 -0.2
25,477 + +0.1
25,478 - -0.5
25,478 - +0.7
25,657 + GFF3147 0.13 -0.2
25,658 - GFF3147 0.13 -0.9
25,660 + GFF3147 0.13 +0.4
25,661 - GFF3147 0.13 +1.1
25,661 - GFF3147 0.13 +0.8
25,768 + GFF3147 0.21 +0.5
26,916 + -0.1
26,916 + -1.1
26,917 - +1.0
26,917 - -0.1
26,917 - +0.3
27,337 - GFF3148 0.45 +0.5
27,337 - GFF3148 0.45 -0.7
27,402 + GFF3148 0.52 +0.4
27,402 + GFF3148 0.52 -1.1
27,403 - GFF3148 0.52 -1.7
27,403 - GFF3148 0.52 -0.4

Or see this region's nucleotide sequence