Experiment: Ying_sugar7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4860 and GFF4861 are separated by 53 nucleotides GFF4861 and GFF4862 are separated by 91 nucleotides GFF4862 and GFF4863 are separated by 49 nucleotides
GFF4860 - Outer membrane protein YfgL, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and NlpB), at 1,866,731 to 1,867,837
GFF4860
GFF4861 - Mlr7403 protein, at 1,867,891 to 1,868,556
GFF4861
GFF4862 - Histidyl-tRNA synthetase (EC 6.1.1.21), at 1,868,648 to 1,869,952
GFF4862
GFF4863 - 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1), at 1,870,002 to 1,871,288
GFF4863
Position (kb)
1868
1869
1870 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1868.003 kb on + strand, within GFF4861 at 1868.003 kb on + strand, within GFF4861 at 1868.177 kb on + strand, within GFF4861 at 1868.177 kb on + strand, within GFF4861 at 1868.177 kb on + strand, within GFF4861 at 1868.177 kb on + strand, within GFF4861 at 1868.178 kb on - strand, within GFF4861 at 1868.178 kb on - strand, within GFF4861 at 1868.178 kb on - strand, within GFF4861 at 1868.178 kb on - strand, within GFF4861 at 1868.420 kb on + strand, within GFF4861 at 1868.606 kb on + strand at 1868.606 kb on + strand at 1868.607 kb on - strand at 1868.607 kb on - strand at 1868.607 kb on - strand at 1868.607 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3 remove 1,868,003 + GFF4861 0.17 -0.2 1,868,003 + GFF4861 0.17 -1.0 1,868,177 + GFF4861 0.43 -1.7 1,868,177 + GFF4861 0.43 -2.4 1,868,177 + GFF4861 0.43 -0.0 1,868,177 + GFF4861 0.43 -0.4 1,868,178 - GFF4861 0.43 +0.2 1,868,178 - GFF4861 0.43 -1.9 1,868,178 - GFF4861 0.43 -3.7 1,868,178 - GFF4861 0.43 -0.9 1,868,420 + GFF4861 0.79 -1.4 1,868,606 + -0.0 1,868,606 + -0.1 1,868,607 - -2.0 1,868,607 - +0.1 1,868,607 - +0.6 1,868,607 - +1.0
Or see this region's nucleotide sequence