Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF4460

Experiment: Ying_sugar7 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4458 and GFF4459 overlap by 4 nucleotidesGFF4459 and GFF4460 are separated by 15 nucleotidesGFF4460 and GFF4461 overlap by 4 nucleotidesGFF4461 and GFF4462 overlap by 1 nucleotides GFF4458 - Histidine kinase, at 1,386,396 to 1,387,739 GFF4458 GFF4459 - Serine-protein kinase RsbW (EC 2.7.11.1), at 1,387,736 to 1,388,173 GFF4459 GFF4460 - Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV, at 1,388,189 to 1,388,533 GFF4460 GFF4461 - UPF0028 protein YchK, at 1,388,530 to 1,389,417 GFF4461 GFF4462 - Serine phosphatase RsbU, regulator of sigma subunit, at 1,389,417 to 1,390,625 GFF4462 Position (kb) 1388 1389Strain fitness (log2 ratio) -1 0 1at 1387.735 kb on + strandat 1387.735 kb on + strandat 1387.736 kb on - strandat 1388.103 kb on - strand, within GFF4459at 1388.342 kb on + strand, within GFF4460at 1388.343 kb on - strand, within GFF4460at 1388.343 kb on - strand, within GFF4460at 1388.487 kb on - strand, within GFF4460at 1389.058 kb on + strand, within GFF4461at 1389.058 kb on + strand, within GFF4461at 1389.317 kb on - strand, within GFF4461at 1389.317 kb on - strand, within GFF4461at 1389.361 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3
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1,387,735 + -1.3
1,387,735 + -0.3
1,387,736 - +0.5
1,388,103 - GFF4459 0.84 -0.2
1,388,342 + GFF4460 0.44 -0.4
1,388,343 - GFF4460 0.45 -0.8
1,388,343 - GFF4460 0.45 +0.5
1,388,487 - GFF4460 0.86 +0.1
1,389,058 + GFF4461 0.59 -0.3
1,389,058 + GFF4461 0.59 -0.8
1,389,317 - GFF4461 0.89 -0.3
1,389,317 - GFF4461 0.89 -0.3
1,389,361 + +1.0

Or see this region's nucleotide sequence