Experiment: Ying_sugar7 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1219 and GFF1220 are separated by 54 nucleotides GFF1220 and GFF1221 are separated by 17 nucleotides GFF1221 and GFF1222 overlap by 4 nucleotides
GFF1219 - putative; ORF located using Glimmer/Genemark, at 84,140 to 84,865
GFF1219
GFF1220 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 84,920 to 85,771
GFF1220
GFF1221 - hypothetical protein, at 85,789 to 86,031
GFF1221
GFF1222 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 86,028 to 87,050
GFF1222
Position (kb) 85
86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 84.809 kb on + strand at 84.809 kb on + strand at 84.809 kb on + strand at 84.809 kb on + strand at 84.810 kb on - strand at 84.810 kb on - strand at 84.810 kb on - strand at 86.030 kb on - strand at 86.179 kb on + strand, within GFF1222 at 86.179 kb on + strand, within GFF1222 at 86.179 kb on + strand, within GFF1222 at 86.180 kb on - strand, within GFF1222 at 86.180 kb on - strand, within GFF1222 at 86.503 kb on + strand, within GFF1222 at 86.503 kb on + strand, within GFF1222 at 86.503 kb on + strand, within GFF1222 at 86.503 kb on + strand, within GFF1222 at 86.899 kb on + strand, within GFF1222 at 86.899 kb on + strand, within GFF1222 at 86.899 kb on + strand, within GFF1222
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep C; time point 3 remove 84,809 + +0.2 84,809 + +0.7 84,809 + -0.0 84,809 + -1.4 84,810 - +0.3 84,810 - +0.5 84,810 - +0.2 86,030 - +1.0 86,179 + GFF1222 0.15 -3.0 86,179 + GFF1222 0.15 -1.6 86,179 + GFF1222 0.15 -1.6 86,180 - GFF1222 0.15 -2.8 86,180 - GFF1222 0.15 -2.2 86,503 + GFF1222 0.46 -2.4 86,503 + GFF1222 0.46 -2.9 86,503 + GFF1222 0.46 -2.2 86,503 + GFF1222 0.46 -3.4 86,899 + GFF1222 0.85 -2.0 86,899 + GFF1222 0.85 -3.6 86,899 + GFF1222 0.85 -2.1
Or see this region's nucleotide sequence