Experiment: Ying_sugar7 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF982 and GFF983 are separated by 173 nucleotides GFF983 and GFF984 are separated by 90 nucleotides GFF984 and GFF985 overlap by 15 nucleotides GFF985 and GFF986 are separated by 10 nucleotides GFF986 and GFF987 are separated by 25 nucleotides
GFF982 - Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4), at 722,060 to 723,049
GFF982
GFF983 - hypothetical protein, at 723,223 to 723,336
GFF983
GFF984 - hypothetical protein, at 723,427 to 723,681
GFF984
GFF985 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 723,667 to 725,262
GFF985
GFF986 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 725,273 to 726,070
GFF986
GFF987 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 726,096 to 728,120
GFF987
Position (kb) 723
724
725
726 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 722.748 kb on - strand, within GFF982 at 723.322 kb on - strand, within GFF983 at 723.322 kb on - strand, within GFF983 at 723.326 kb on - strand at 723.373 kb on + strand at 723.374 kb on - strand at 723.374 kb on - strand at 723.374 kb on - strand at 723.387 kb on - strand at 723.501 kb on + strand, within GFF984 at 723.910 kb on + strand, within GFF985 at 723.910 kb on + strand, within GFF985 at 723.910 kb on + strand, within GFF985 at 724.043 kb on + strand, within GFF985 at 724.043 kb on + strand, within GFF985 at 724.043 kb on + strand, within GFF985 at 724.044 kb on - strand, within GFF985 at 724.088 kb on - strand, within GFF985 at 724.514 kb on - strand, within GFF985 at 724.514 kb on - strand, within GFF985 at 724.638 kb on + strand, within GFF985 at 724.638 kb on + strand, within GFF985 at 724.638 kb on + strand, within GFF985 at 724.638 kb on + strand, within GFF985 at 724.639 kb on - strand, within GFF985 at 724.767 kb on - strand, within GFF985 at 724.858 kb on + strand, within GFF985 at 724.859 kb on - strand, within GFF985 at 724.859 kb on - strand, within GFF985 at 724.859 kb on - strand, within GFF985 at 724.859 kb on - strand, within GFF985 at 724.859 kb on - strand, within GFF985 at 724.859 kb on - strand, within GFF985 at 724.927 kb on + strand, within GFF985 at 724.928 kb on - strand, within GFF985 at 724.928 kb on - strand, within GFF985 at 724.928 kb on - strand, within GFF985 at 724.928 kb on - strand, within GFF985 at 725.096 kb on + strand, within GFF985 at 725.107 kb on + strand at 725.131 kb on + strand at 725.132 kb on - strand at 725.132 kb on - strand at 725.252 kb on - strand at 725.359 kb on + strand, within GFF986 at 725.581 kb on + strand, within GFF986 at 725.582 kb on - strand, within GFF986 at 725.582 kb on - strand, within GFF986 at 725.679 kb on - strand, within GFF986 at 725.933 kb on + strand, within GFF986 at 725.933 kb on + strand, within GFF986 at 725.934 kb on - strand, within GFF986 at 725.934 kb on - strand, within GFF986 at 725.934 kb on - strand, within GFF986 at 725.969 kb on + strand, within GFF986 at 725.969 kb on + strand, within GFF986 at 726.102 kb on - strand at 726.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 3 remove 722,748 - GFF982 0.69 +0.8 723,322 - GFF983 0.87 -1.2 723,322 - GFF983 0.87 +0.2 723,326 - +0.4 723,373 + -0.2 723,374 - +0.3 723,374 - +2.8 723,374 - +0.3 723,387 - +0.4 723,501 + GFF984 0.29 -0.2 723,910 + GFF985 0.15 -0.7 723,910 + GFF985 0.15 +0.1 723,910 + GFF985 0.15 -0.2 724,043 + GFF985 0.24 +0.1 724,043 + GFF985 0.24 +0.2 724,043 + GFF985 0.24 -0.5 724,044 - GFF985 0.24 -1.7 724,088 - GFF985 0.26 -1.5 724,514 - GFF985 0.53 -0.8 724,514 - GFF985 0.53 +0.3 724,638 + GFF985 0.61 +0.3 724,638 + GFF985 0.61 -2.0 724,638 + GFF985 0.61 +0.4 724,638 + GFF985 0.61 -0.0 724,639 - GFF985 0.61 -0.4 724,767 - GFF985 0.69 +0.2 724,858 + GFF985 0.75 +0.0 724,859 - GFF985 0.75 +0.1 724,859 - GFF985 0.75 -0.4 724,859 - GFF985 0.75 +0.5 724,859 - GFF985 0.75 +0.7 724,859 - GFF985 0.75 -1.2 724,859 - GFF985 0.75 +0.1 724,927 + GFF985 0.79 -0.5 724,928 - GFF985 0.79 -0.2 724,928 - GFF985 0.79 -0.2 724,928 - GFF985 0.79 -0.4 724,928 - GFF985 0.79 -0.1 725,096 + GFF985 0.90 +0.5 725,107 + +0.0 725,131 + +0.6 725,132 - +0.6 725,132 - -1.7 725,252 - -0.2 725,359 + GFF986 0.11 -1.7 725,581 + GFF986 0.39 +0.2 725,582 - GFF986 0.39 +0.3 725,582 - GFF986 0.39 -0.3 725,679 - GFF986 0.51 +0.8 725,933 + GFF986 0.83 +0.5 725,933 + GFF986 0.83 -0.5 725,934 - GFF986 0.83 -0.5 725,934 - GFF986 0.83 -0.5 725,934 - GFF986 0.83 -0.6 725,969 + GFF986 0.87 -0.4 725,969 + GFF986 0.87 +0.7 726,102 - +1.1 726,102 - +0.1
Or see this region's nucleotide sequence