Experiment: Ying_sugar7 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3642 and GFF3643 overlap by 10 nucleotides GFF3643 and GFF3644 overlap by 4 nucleotides GFF3644 and GFF3645 overlap by 4 nucleotides
GFF3642 - histidine kinase, at 525,733 to 527,007
GFF3642
GFF3643 - hypothetical protein, at 526,998 to 528,149
GFF3643
GFF3644 - Ribonuclease BN (EC 3.1.-.-), at 528,146 to 529,000
GFF3644
GFF3645 - CBS domain protein, at 528,997 to 529,428
GFF3645
Position (kb) 526
527
528
529 Strain fitness (log2 ratio) -2
-1
0
1
2 at 526.187 kb on - strand, within GFF3642 at 526.876 kb on + strand, within GFF3642 at 526.876 kb on + strand, within GFF3642 at 526.876 kb on + strand, within GFF3642 at 526.876 kb on + strand, within GFF3642 at 526.877 kb on - strand, within GFF3642 at 526.877 kb on - strand, within GFF3642 at 526.877 kb on - strand, within GFF3642 at 527.000 kb on - strand at 527.000 kb on - strand at 527.120 kb on - strand, within GFF3643 at 527.444 kb on - strand, within GFF3643 at 527.546 kb on - strand, within GFF3643 at 528.064 kb on + strand at 528.113 kb on + strand at 528.234 kb on + strand, within GFF3644 at 528.309 kb on + strand, within GFF3644 at 528.310 kb on - strand, within GFF3644 at 528.310 kb on - strand, within GFF3644 at 528.310 kb on - strand, within GFF3644 at 528.410 kb on + strand, within GFF3644 at 528.918 kb on + strand at 528.918 kb on + strand at 528.919 kb on - strand at 528.919 kb on - strand at 528.921 kb on + strand at 528.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 3 remove 526,187 - GFF3642 0.36 +0.4 526,876 + GFF3642 0.90 +0.8 526,876 + GFF3642 0.90 +0.4 526,876 + GFF3642 0.90 +0.0 526,876 + GFF3642 0.90 -0.2 526,877 - GFF3642 0.90 -2.3 526,877 - GFF3642 0.90 -0.2 526,877 - GFF3642 0.90 +0.6 527,000 - +0.0 527,000 - +2.2 527,120 - GFF3643 0.11 -0.3 527,444 - GFF3643 0.39 -0.8 527,546 - GFF3643 0.48 +0.2 528,064 + -0.0 528,113 + +1.1 528,234 + GFF3644 0.10 +0.0 528,309 + GFF3644 0.19 +0.0 528,310 - GFF3644 0.19 -0.1 528,310 - GFF3644 0.19 +0.3 528,310 - GFF3644 0.19 -0.6 528,410 + GFF3644 0.31 -0.5 528,918 + -1.2 528,918 + +0.2 528,919 - -0.8 528,919 - +0.3 528,921 + -0.2 528,922 - +0.8
Or see this region's nucleotide sequence