Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF2

Experiment: Ying_sugar7 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1 and GFF2 are separated by 6 nucleotidesGFF2 and GFF3 are separated by 347 nucleotides GFF1 - Phenylacetate-coenzyme A ligase (EC 6.2.1.30), at 4 to 183 GFF1 GFF2 - hypothetical protein, at 190 to 966 GFF2 GFF3 - hypothetical protein, at 1,314 to 1,637 GFF3 Position (kb) 0 1Strain fitness (log2 ratio) -1 0 1 2at 0.236 kb on + strandat 0.236 kb on + strandat 0.236 kb on + strandat 0.237 kb on + strandat 0.279 kb on - strand, within GFF2at 0.341 kb on + strand, within GFF2at 0.341 kb on + strand, within GFF2at 0.342 kb on - strand, within GFF2at 0.342 kb on - strand, within GFF2at 0.389 kb on + strand, within GFF2at 0.389 kb on + strand, within GFF2at 0.390 kb on - strand, within GFF2at 0.390 kb on - strand, within GFF2at 0.390 kb on - strand, within GFF2at 0.390 kb on - strand, within GFF2at 0.993 kb on + strandat 0.993 kb on + strandat 0.993 kb on + strandat 0.993 kb on + strandat 0.993 kb on + strandat 0.994 kb on - strandat 0.994 kb on - strandat 0.994 kb on - strandat 0.994 kb on - strandat 1.149 kb on + strandat 1.149 kb on + strandat 1.150 kb on - strandat 1.150 kb on - strandat 1.390 kb on + strand, within GFF3at 1.390 kb on + strand, within GFF3at 1.391 kb on - strand, within GFF3at 1.407 kb on + strand, within GFF3at 1.407 kb on + strand, within GFF3at 1.407 kb on + strand, within GFF3at 1.408 kb on - strand, within GFF3at 1.408 kb on - strand, within GFF3at 1.408 kb on - strand, within GFF3at 1.408 kb on - strand, within GFF3at 1.408 kb on - strand, within GFF3at 1.476 kb on + strand, within GFF3at 1.476 kb on + strand, within GFF3at 1.509 kb on + strand, within GFF3at 1.509 kb on + strand, within GFF3at 1.509 kb on + strand, within GFF3at 1.509 kb on + strand, within GFF3at 1.510 kb on - strand, within GFF3at 1.510 kb on - strand, within GFF3at 1.510 kb on - strand, within GFF3at 1.510 kb on - strand, within GFF3at 1.510 kb on - strand, within GFF3at 1.510 kb on - strand, within GFF3at 1.510 kb on - strand, within GFF3at 1.560 kb on + strand, within GFF3at 1.561 kb on - strand, within GFF3at 1.561 kb on - strand, within GFF3at 1.561 kb on - strand, within GFF3at 1.786 kb on + strandat 1.787 kb on - strandat 1.871 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 3
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236 + -0.5
236 + -0.2
236 + +0.7
237 + -1.0
279 - GFF2 0.11 +0.5
341 + GFF2 0.19 -0.3
341 + GFF2 0.19 +0.0
342 - GFF2 0.20 +0.1
342 - GFF2 0.20 +0.6
389 + GFF2 0.26 +0.3
389 + GFF2 0.26 +0.1
390 - GFF2 0.26 +0.0
390 - GFF2 0.26 -0.6
390 - GFF2 0.26 +0.5
390 - GFF2 0.26 +0.1
993 + +0.6
993 + +0.2
993 + -0.2
993 + -0.2
993 + -0.7
994 - +0.1
994 - +0.9
994 - +0.4
994 - +0.2
1,149 + -0.2
1,149 + +0.9
1,150 - +0.2
1,150 - -0.0
1,390 + GFF3 0.23 +0.2
1,390 + GFF3 0.23 +0.7
1,391 - GFF3 0.24 -0.3
1,407 + GFF3 0.29 -0.5
1,407 + GFF3 0.29 +0.8
1,407 + GFF3 0.29 -0.0
1,408 - GFF3 0.29 -0.2
1,408 - GFF3 0.29 +1.0
1,408 - GFF3 0.29 -0.2
1,408 - GFF3 0.29 +0.3
1,408 - GFF3 0.29 -0.2
1,476 + GFF3 0.50 -0.6
1,476 + GFF3 0.50 -0.6
1,509 + GFF3 0.60 -0.0
1,509 + GFF3 0.60 +1.1
1,509 + GFF3 0.60 -0.6
1,509 + GFF3 0.60 +0.0
1,510 - GFF3 0.60 -0.5
1,510 - GFF3 0.60 -0.4
1,510 - GFF3 0.60 -0.1
1,510 - GFF3 0.60 +0.6
1,510 - GFF3 0.60 -0.4
1,510 - GFF3 0.60 +0.3
1,510 - GFF3 0.60 -0.0
1,560 + GFF3 0.76 +0.0
1,561 - GFF3 0.76 +2.1
1,561 - GFF3 0.76 +0.9
1,561 - GFF3 0.76 +0.0
1,786 + -0.5
1,787 - -0.2
1,871 - +0.3

Or see this region's nucleotide sequence