Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF516

Experiment: Ying_sugar7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF514 and GFF515 are separated by 3 nucleotidesGFF515 and GFF516 are separated by 7 nucleotidesGFF516 and GFF517 are separated by 38 nucleotides GFF514 - Esterase/lipase, at 181,816 to 182,778 GFF514 GFF515 - Glutamine amidotransferase class-I (EC 6.3.5.2), at 182,782 to 183,522 GFF515 GFF516 - Acyl-CoA hydrolase (EC 3.1.2.20), at 183,530 to 183,970 GFF516 GFF517 - Lipid A export ATP-binding/permease protein MsbA, at 184,009 to 185,856 GFF517 Position (kb) 183 184Strain fitness (log2 ratio) -2 -1 0 1at 182.562 kb on + strand, within GFF514at 182.639 kb on - strand, within GFF514at 182.651 kb on + strand, within GFF514at 182.652 kb on - strand, within GFF514at 182.652 kb on - strand, within GFF514at 182.921 kb on - strand, within GFF515at 183.205 kb on + strand, within GFF515at 183.205 kb on + strand, within GFF515at 183.206 kb on - strand, within GFF515at 183.380 kb on - strand, within GFF515at 183.380 kb on - strand, within GFF515at 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.607 kb on - strand, within GFF516at 183.607 kb on - strand, within GFF516at 183.607 kb on - strand, within GFF516at 183.636 kb on + strand, within GFF516at 183.636 kb on + strand, within GFF516at 183.804 kb on + strand, within GFF516at 183.804 kb on + strand, within GFF516at 184.124 kb on - strandat 184.135 kb on + strandat 184.136 kb on - strandat 184.504 kb on + strand, within GFF517at 184.505 kb on - strand, within GFF517at 184.514 kb on - strand, within GFF517at 184.586 kb on - strand, within GFF517at 184.618 kb on + strand, within GFF517at 184.618 kb on + strand, within GFF517at 184.618 kb on + strand, within GFF517at 184.619 kb on - strand, within GFF517at 184.619 kb on - strand, within GFF517at 184.619 kb on - strand, within GFF517at 184.726 kb on + strand, within GFF517at 184.726 kb on + strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.741 kb on + strand, within GFF517at 184.741 kb on + strand, within GFF517at 184.741 kb on + strand, within GFF517at 184.742 kb on - strand, within GFF517at 184.742 kb on - strand, within GFF517at 184.969 kb on + strand, within GFF517at 184.969 kb on + strand, within GFF517

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 3
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182,562 + GFF514 0.77 +0.0
182,639 - GFF514 0.85 -0.9
182,651 + GFF514 0.87 +0.2
182,652 - GFF514 0.87 +0.2
182,652 - GFF514 0.87 +0.1
182,921 - GFF515 0.19 +0.8
183,205 + GFF515 0.57 -0.7
183,205 + GFF515 0.57 -1.0
183,206 - GFF515 0.57 -0.2
183,380 - GFF515 0.81 -0.6
183,380 - GFF515 0.81 +0.4
183,487 + +0.4
183,487 + +0.6
183,487 + -0.7
183,487 + -0.2
183,487 + +0.5
183,607 - GFF516 0.17 +0.3
183,607 - GFF516 0.17 -1.4
183,607 - GFF516 0.17 -0.9
183,636 + GFF516 0.24 +1.6
183,636 + GFF516 0.24 -0.2
183,804 + GFF516 0.62 -0.2
183,804 + GFF516 0.62 -2.6
184,124 - -0.2
184,135 + +0.3
184,136 - -0.1
184,504 + GFF517 0.27 +0.4
184,505 - GFF517 0.27 +1.0
184,514 - GFF517 0.27 +0.6
184,586 - GFF517 0.31 +0.2
184,618 + GFF517 0.33 +0.3
184,618 + GFF517 0.33 -0.1
184,618 + GFF517 0.33 +0.3
184,619 - GFF517 0.33 +0.1
184,619 - GFF517 0.33 +0.2
184,619 - GFF517 0.33 +0.1
184,726 + GFF517 0.39 +0.9
184,726 + GFF517 0.39 +0.6
184,727 - GFF517 0.39 -0.3
184,727 - GFF517 0.39 -0.3
184,727 - GFF517 0.39 -0.7
184,727 - GFF517 0.39 +0.5
184,727 - GFF517 0.39 -0.0
184,741 + GFF517 0.40 -0.7
184,741 + GFF517 0.40 +0.8
184,741 + GFF517 0.40 -0.5
184,742 - GFF517 0.40 +0.6
184,742 - GFF517 0.40 +0.4
184,969 + GFF517 0.52 -1.4
184,969 + GFF517 0.52 -0.4

Or see this region's nucleotide sequence