Experiment: Ying_sugar7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF480 and GFF481 are separated by 45 nucleotides GFF481 and GFF482 overlap by 4 nucleotides GFF482 and GFF483 are separated by 5 nucleotides
GFF480 - Geranyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.5), at 143,866 to 145,413
GFF480
GFF481 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 145,459 to 146,631
GFF481
GFF482 - Enoyl-CoA hydratase (EC 4.2.1.17), at 146,628 to 147,428
GFF482
GFF483 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 147,434 to 149,341
GFF483
Position (kb) 145
146
147 Strain fitness (log2 ratio) -2
-1
0
1 at 144.545 kb on - strand, within GFF480 at 144.911 kb on - strand, within GFF480 at 144.931 kb on + strand, within GFF480 at 144.932 kb on - strand, within GFF480 at 144.932 kb on - strand, within GFF480 at 144.932 kb on - strand, within GFF480 at 145.061 kb on - strand, within GFF480 at 145.412 kb on - strand at 145.507 kb on + strand at 145.508 kb on - strand at 145.652 kb on - strand, within GFF481 at 145.652 kb on - strand, within GFF481 at 145.657 kb on + strand, within GFF481 at 145.657 kb on + strand, within GFF481 at 146.111 kb on - strand, within GFF481 at 146.173 kb on + strand, within GFF481 at 146.372 kb on - strand, within GFF481 at 146.390 kb on - strand, within GFF481 at 146.736 kb on + strand, within GFF482
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 3 remove 144,545 - GFF480 0.44 +0.9 144,911 - GFF480 0.68 +0.2 144,931 + GFF480 0.69 -1.8 144,932 - GFF480 0.69 +0.1 144,932 - GFF480 0.69 -0.0 144,932 - GFF480 0.69 +0.1 145,061 - GFF480 0.77 +0.0 145,412 - +0.4 145,507 + +0.2 145,508 - +0.2 145,652 - GFF481 0.16 +0.6 145,652 - GFF481 0.16 +0.4 145,657 + GFF481 0.17 +1.1 145,657 + GFF481 0.17 +1.0 146,111 - GFF481 0.56 +0.6 146,173 + GFF481 0.61 -0.2 146,372 - GFF481 0.78 +0.0 146,390 - GFF481 0.79 -0.0 146,736 + GFF482 0.13 +0.3
Or see this region's nucleotide sequence