Experiment: Ying_sugar7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4298 and GFF4299 are separated by 83 nucleotides GFF4299 and GFF4300 are separated by 24 nucleotides GFF4300 and GFF4301 are separated by 74 nucleotides
GFF4298 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 1,223,232 to 1,224,002
GFF4298
GFF4299 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 1,224,086 to 1,224,580
GFF4299
GFF4300 - N-acetylglutamate synthase (EC 2.3.1.1), at 1,224,605 to 1,225,945
GFF4300
GFF4301 - macromolecule metabolism; macromolecule synthesis, modification; dna - replication, repair, restr./modif., at 1,226,020 to 1,229,898
GFF4301
Position (kb)
1224
1225
1226 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1224.164 kb on + strand, within GFF4299 at 1224.164 kb on + strand, within GFF4299 at 1224.164 kb on + strand, within GFF4299 at 1224.164 kb on + strand, within GFF4299 at 1224.165 kb on - strand, within GFF4299 at 1224.165 kb on - strand, within GFF4299 at 1224.218 kb on + strand, within GFF4299 at 1224.218 kb on + strand, within GFF4299 at 1224.218 kb on + strand, within GFF4299 at 1224.218 kb on + strand, within GFF4299 at 1224.218 kb on + strand, within GFF4299 at 1224.218 kb on + strand, within GFF4299 at 1224.219 kb on - strand, within GFF4299 at 1224.284 kb on + strand, within GFF4299 at 1224.285 kb on - strand, within GFF4299 at 1224.285 kb on - strand, within GFF4299 at 1224.285 kb on - strand, within GFF4299 at 1224.651 kb on + strand at 1224.651 kb on + strand at 1224.652 kb on - strand at 1224.652 kb on - strand at 1224.652 kb on - strand at 1224.652 kb on - strand at 1224.652 kb on - strand at 1224.873 kb on + strand, within GFF4300 at 1224.874 kb on - strand, within GFF4300 at 1224.879 kb on + strand, within GFF4300 at 1224.880 kb on - strand, within GFF4300 at 1224.880 kb on - strand, within GFF4300 at 1224.927 kb on + strand, within GFF4300 at 1224.927 kb on + strand, within GFF4300 at 1224.927 kb on + strand, within GFF4300 at 1224.928 kb on - strand, within GFF4300 at 1224.928 kb on - strand, within GFF4300 at 1225.095 kb on + strand, within GFF4300 at 1225.095 kb on + strand, within GFF4300 at 1225.096 kb on - strand, within GFF4300 at 1225.096 kb on - strand, within GFF4300 at 1225.096 kb on - strand, within GFF4300 at 1225.096 kb on - strand, within GFF4300 at 1225.096 kb on - strand, within GFF4300 at 1225.096 kb on - strand, within GFF4300 at 1225.096 kb on - strand, within GFF4300 at 1225.188 kb on + strand, within GFF4300 at 1225.188 kb on + strand, within GFF4300 at 1225.188 kb on + strand, within GFF4300 at 1225.188 kb on + strand, within GFF4300 at 1225.189 kb on - strand, within GFF4300 at 1225.701 kb on + strand, within GFF4300 at 1225.701 kb on + strand, within GFF4300 at 1225.701 kb on + strand, within GFF4300 at 1225.702 kb on - strand, within GFF4300 at 1225.739 kb on + strand, within GFF4300 at 1225.739 kb on + strand, within GFF4300 at 1225.891 kb on - strand at 1226.320 kb on + strand at 1226.320 kb on + strand at 1226.320 kb on + strand at 1226.321 kb on - strand at 1226.321 kb on - strand at 1226.321 kb on - strand at 1226.425 kb on + strand, within GFF4301 at 1226.425 kb on + strand, within GFF4301 at 1226.488 kb on + strand, within GFF4301 at 1226.489 kb on - strand, within GFF4301 at 1226.573 kb on - strand, within GFF4301 at 1226.671 kb on + strand, within GFF4301 at 1226.672 kb on - strand, within GFF4301 at 1226.672 kb on - strand, within GFF4301
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 3 remove 1,224,164 + GFF4299 0.16 -1.0 1,224,164 + GFF4299 0.16 -0.8 1,224,164 + GFF4299 0.16 -1.4 1,224,164 + GFF4299 0.16 +0.7 1,224,165 - GFF4299 0.16 -0.7 1,224,165 - GFF4299 0.16 -0.9 1,224,218 + GFF4299 0.27 -0.5 1,224,218 + GFF4299 0.27 -0.7 1,224,218 + GFF4299 0.27 -1.8 1,224,218 + GFF4299 0.27 -1.2 1,224,218 + GFF4299 0.27 -1.5 1,224,218 + GFF4299 0.27 -1.3 1,224,219 - GFF4299 0.27 -0.3 1,224,284 + GFF4299 0.40 -0.9 1,224,285 - GFF4299 0.40 -0.8 1,224,285 - GFF4299 0.40 -0.5 1,224,285 - GFF4299 0.40 -1.5 1,224,651 + -3.3 1,224,651 + -4.8 1,224,652 - -2.9 1,224,652 - -3.9 1,224,652 - -3.1 1,224,652 - -3.4 1,224,652 - -2.3 1,224,873 + GFF4300 0.20 -4.0 1,224,874 - GFF4300 0.20 -1.5 1,224,879 + GFF4300 0.20 -1.9 1,224,880 - GFF4300 0.21 -2.3 1,224,880 - GFF4300 0.21 -2.3 1,224,927 + GFF4300 0.24 -3.6 1,224,927 + GFF4300 0.24 -3.6 1,224,927 + GFF4300 0.24 -0.9 1,224,928 - GFF4300 0.24 -5.8 1,224,928 - GFF4300 0.24 -3.5 1,225,095 + GFF4300 0.37 -2.1 1,225,095 + GFF4300 0.37 -1.3 1,225,096 - GFF4300 0.37 -3.8 1,225,096 - GFF4300 0.37 -0.9 1,225,096 - GFF4300 0.37 -2.5 1,225,096 - GFF4300 0.37 -1.3 1,225,096 - GFF4300 0.37 -1.3 1,225,096 - GFF4300 0.37 -2.7 1,225,096 - GFF4300 0.37 -3.2 1,225,188 + GFF4300 0.43 -3.5 1,225,188 + GFF4300 0.43 -3.1 1,225,188 + GFF4300 0.43 -1.3 1,225,188 + GFF4300 0.43 -0.9 1,225,189 - GFF4300 0.44 -2.9 1,225,701 + GFF4300 0.82 -3.5 1,225,701 + GFF4300 0.82 -4.0 1,225,701 + GFF4300 0.82 -4.7 1,225,702 - GFF4300 0.82 -2.1 1,225,739 + GFF4300 0.85 -3.5 1,225,739 + GFF4300 0.85 -2.9 1,225,891 - -1.3 1,226,320 + -0.9 1,226,320 + -0.8 1,226,320 + -0.5 1,226,321 - -0.8 1,226,321 - -0.4 1,226,321 - -0.2 1,226,425 + GFF4301 0.10 -0.3 1,226,425 + GFF4301 0.10 -1.6 1,226,488 + GFF4301 0.12 +0.1 1,226,489 - GFF4301 0.12 -0.6 1,226,573 - GFF4301 0.14 +1.0 1,226,671 + GFF4301 0.17 -2.1 1,226,672 - GFF4301 0.17 +0.3 1,226,672 - GFF4301 0.17 +0.2
Or see this region's nucleotide sequence