Experiment: Ying_sugar7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3066 and GFF3067 overlap by 4 nucleotides GFF3067 and GFF3068 are separated by 4 nucleotides GFF3068 and GFF3069 are separated by 85 nucleotides
GFF3066 - LptA, protein essential for LPS transport across the periplasm, at 146,135 to 146,608
GFF3066
GFF3067 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 146,605 to 147,402
GFF3067
GFF3068 - RNA polymerase sigma-54 factor RpoN, at 147,407 to 149,035
GFF3068
GFF3069 - Methyltransferase (EC 2.1.1.-), at 149,121 to 150,449
GFF3069
Position (kb)
147
148
149
150 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 147.962 kb on + strand, within GFF3068 at 147.962 kb on + strand, within GFF3068 at 147.962 kb on + strand, within GFF3068 at 147.963 kb on - strand, within GFF3068 at 147.996 kb on - strand, within GFF3068 at 147.996 kb on - strand, within GFF3068 at 147.996 kb on - strand, within GFF3068 at 148.493 kb on + strand, within GFF3068 at 148.493 kb on + strand, within GFF3068 at 148.493 kb on + strand, within GFF3068 at 148.494 kb on - strand, within GFF3068 at 148.494 kb on - strand, within GFF3068 at 148.769 kb on + strand, within GFF3068 at 148.769 kb on + strand, within GFF3068 at 148.769 kb on + strand, within GFF3068 at 148.769 kb on + strand, within GFF3068 at 148.769 kb on + strand, within GFF3068 at 148.769 kb on + strand, within GFF3068 at 148.769 kb on + strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.770 kb on - strand, within GFF3068 at 148.793 kb on + strand, within GFF3068 at 148.805 kb on + strand, within GFF3068 at 148.805 kb on + strand, within GFF3068 at 148.805 kb on + strand, within GFF3068 at 148.805 kb on + strand, within GFF3068 at 148.806 kb on - strand, within GFF3068 at 148.806 kb on - strand, within GFF3068 at 148.985 kb on + strand at 148.985 kb on + strand at 148.985 kb on + strand at 148.985 kb on + strand at 148.985 kb on + strand at 149.031 kb on + strand at 149.031 kb on + strand at 149.031 kb on + strand at 149.032 kb on - strand at 149.120 kb on + strand at 149.121 kb on - strand at 149.121 kb on - strand at 149.214 kb on + strand at 149.214 kb on + strand at 149.215 kb on - strand at 149.215 kb on - strand at 149.346 kb on + strand, within GFF3069 at 149.347 kb on - strand, within GFF3069 at 149.347 kb on - strand, within GFF3069 at 149.347 kb on - strand, within GFF3069 at 149.547 kb on + strand, within GFF3069 at 149.547 kb on + strand, within GFF3069 at 149.548 kb on - strand, within GFF3069 at 149.548 kb on - strand, within GFF3069 at 149.548 kb on - strand, within GFF3069 at 149.580 kb on + strand, within GFF3069 at 149.580 kb on + strand, within GFF3069 at 149.580 kb on + strand, within GFF3069 at 149.733 kb on + strand, within GFF3069 at 149.734 kb on - strand, within GFF3069
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 3 remove 147,962 + GFF3068 0.34 -7.3 147,962 + GFF3068 0.34 -6.1 147,962 + GFF3068 0.34 -4.8 147,963 - GFF3068 0.34 -5.8 147,996 - GFF3068 0.36 -7.8 147,996 - GFF3068 0.36 -6.9 147,996 - GFF3068 0.36 -2.6 148,493 + GFF3068 0.67 -2.7 148,493 + GFF3068 0.67 -3.5 148,493 + GFF3068 0.67 -4.9 148,494 - GFF3068 0.67 -3.8 148,494 - GFF3068 0.67 -3.6 148,769 + GFF3068 0.84 -3.0 148,769 + GFF3068 0.84 -3.5 148,769 + GFF3068 0.84 -2.1 148,769 + GFF3068 0.84 -5.4 148,769 + GFF3068 0.84 -5.4 148,769 + GFF3068 0.84 -4.9 148,769 + GFF3068 0.84 -4.5 148,770 - GFF3068 0.84 -6.1 148,770 - GFF3068 0.84 -5.3 148,770 - GFF3068 0.84 -5.5 148,770 - GFF3068 0.84 -5.3 148,770 - GFF3068 0.84 -4.7 148,770 - GFF3068 0.84 -5.0 148,770 - GFF3068 0.84 -4.9 148,770 - GFF3068 0.84 -4.5 148,770 - GFF3068 0.84 -4.0 148,770 - GFF3068 0.84 -2.4 148,770 - GFF3068 0.84 -5.1 148,770 - GFF3068 0.84 -3.7 148,793 + GFF3068 0.85 -6.2 148,805 + GFF3068 0.86 -2.3 148,805 + GFF3068 0.86 -4.8 148,805 + GFF3068 0.86 -3.1 148,805 + GFF3068 0.86 -3.3 148,806 - GFF3068 0.86 -6.2 148,806 - GFF3068 0.86 -6.7 148,985 + -2.7 148,985 + -2.0 148,985 + -5.6 148,985 + -5.5 148,985 + -6.1 149,031 + -1.3 149,031 + -1.3 149,031 + -1.1 149,032 - -2.3 149,120 + +0.2 149,121 - -0.3 149,121 - -0.8 149,214 + -0.2 149,214 + -0.3 149,215 - -0.3 149,215 - -0.6 149,346 + GFF3069 0.17 -0.1 149,347 - GFF3069 0.17 -1.3 149,347 - GFF3069 0.17 +0.5 149,347 - GFF3069 0.17 +0.4 149,547 + GFF3069 0.32 +1.0 149,547 + GFF3069 0.32 +0.6 149,548 - GFF3069 0.32 -0.1 149,548 - GFF3069 0.32 -0.4 149,548 - GFF3069 0.32 +0.2 149,580 + GFF3069 0.35 -0.1 149,580 + GFF3069 0.35 -0.1 149,580 + GFF3069 0.35 +0.0 149,733 + GFF3069 0.46 -0.1 149,734 - GFF3069 0.46 -0.3
Or see this region's nucleotide sequence