Experiment: Ying_sugar7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF176 and GFF177 are separated by 24 nucleotides GFF177 and GFF178 overlap by 14 nucleotides GFF178 and GFF179 are separated by 7 nucleotides GFF179 and GFF180 are separated by 85 nucleotides GFF180 and GFF181 overlap by 27 nucleotides
GFF176 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 190,151 to 191,107
GFF176
GFF177 - Demethylmenaquinone methyltransferase, at 191,132 to 191,791
GFF177
GFF178 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 191,778 to 192,737
GFF178
GFF179 - Small-conductance mechanosensitive channel, at 192,745 to 193,320
GFF179
GFF180 - hypothetical protein, at 193,406 to 193,702
GFF180
GFF181 - Putative enzyme of poly-gamma-glutamate biosynthesis (capsule formation), at 193,676 to 195,007
GFF181
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1 at 190.884 kb on - strand, within GFF176 at 191.006 kb on + strand, within GFF176 at 191.526 kb on - strand, within GFF177 at 191.526 kb on - strand, within GFF177 at 191.778 kb on - strand at 191.884 kb on - strand, within GFF178 at 191.884 kb on - strand, within GFF178 at 191.956 kb on - strand, within GFF178 at 192.634 kb on - strand, within GFF178 at 192.876 kb on - strand, within GFF179 at 193.502 kb on + strand, within GFF180 at 193.503 kb on - strand, within GFF180 at 193.503 kb on - strand, within GFF180
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 3 remove 190,884 - GFF176 0.77 -0.2 191,006 + GFF176 0.89 -0.0 191,526 - GFF177 0.60 -0.4 191,526 - GFF177 0.60 +0.0 191,778 - +0.2 191,884 - GFF178 0.11 +0.7 191,884 - GFF178 0.11 +0.1 191,956 - GFF178 0.19 -0.1 192,634 - GFF178 0.89 +0.6 192,876 - GFF179 0.23 -2.1 193,502 + GFF180 0.32 +0.1 193,503 - GFF180 0.33 -0.4 193,503 - GFF180 0.33 +0.3
Or see this region's nucleotide sequence