Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF2113

Experiment: Ying_OrganicAcid7 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2111 and GFF2112 are separated by 7 nucleotidesGFF2112 and GFF2113 overlap by 4 nucleotidesGFF2113 and GFF2114 are separated by 223 nucleotides GFF2111 - Enoyl-CoA hydratase (EC 4.2.1.17), at 1,000,141 to 1,000,956 GFF2111 GFF2112 - 2-keto-4-pentenoate hydratase (EC 4.2.1.80), at 1,000,964 to 1,001,734 GFF2112 GFF2113 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,001,731 to 1,002,627 GFF2113 GFF2114 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 1,002,851 to 1,003,645 GFF2114 Position (kb) 1001 1002 1003Strain fitness (log2 ratio) -1 0 1at 1001.063 kb on + strand, within GFF2112at 1001.063 kb on + strand, within GFF2112at 1001.063 kb on + strand, within GFF2112at 1001.977 kb on + strand, within GFF2113at 1002.625 kb on + strandat 1002.852 kb on + strandat 1002.852 kb on + strandat 1002.852 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 3
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1,001,063 + GFF2112 0.13 -0.5
1,001,063 + GFF2112 0.13 -0.1
1,001,063 + GFF2112 0.13 +0.6
1,001,977 + GFF2113 0.27 -0.9
1,002,625 + +0.1
1,002,852 + +1.3
1,002,852 + -0.3
1,002,852 + -0.6

Or see this region's nucleotide sequence