Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF2100

Experiment: Ying_OrganicAcid7 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2098 and GFF2099 are separated by 82 nucleotidesGFF2099 and GFF2100 overlap by 4 nucleotidesGFF2100 and GFF2101 are separated by 3 nucleotidesGFF2101 and GFF2102 are separated by 1 nucleotides GFF2098 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 985,618 to 987,507 GFF2098 GFF2099 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 987,590 to 988,366 GFF2099 GFF2100 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 988,363 to 989,079 GFF2100 GFF2101 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 989,083 to 989,958 GFF2101 GFF2102 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 989,960 to 991,042 GFF2102 Position (kb) 988 989 990Strain fitness (log2 ratio) -1 0 1at 987.392 kb on + strandat 987.393 kb on - strandat 987.413 kb on - strandat 987.506 kb on - strandat 987.559 kb on - strandat 988.031 kb on + strand, within GFF2099at 988.347 kb on - strandat 988.675 kb on + strand, within GFF2100at 988.729 kb on + strand, within GFF2100at 988.987 kb on + strand, within GFF2100at 989.260 kb on + strand, within GFF2101at 989.432 kb on - strand, within GFF2101at 989.848 kb on + strand, within GFF2101

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 3
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987,392 + +0.0
987,393 - -0.0
987,413 - -0.5
987,506 - +0.1
987,559 - -0.2
988,031 + GFF2099 0.57 +0.4
988,347 - -1.5
988,675 + GFF2100 0.44 -0.4
988,729 + GFF2100 0.51 -0.0
988,987 + GFF2100 0.87 -0.3
989,260 + GFF2101 0.20 -0.1
989,432 - GFF2101 0.40 -0.6
989,848 + GFF2101 0.87 -0.6

Or see this region's nucleotide sequence