Experiment: Ying_OrganicAcid7 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF183 and GFF184 are separated by 4 nucleotides GFF184 and GFF185 overlap by 4 nucleotides GFF185 and GFF186 are separated by 40 nucleotides GFF186 and GFF187 overlap by 4 nucleotides
GFF183 - Oligopeptide transport ATP-binding protein OppF (TC 3.A.1.5.1), at 196,059 to 196,973
GFF183
GFF184 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 196,978 to 197,931
GFF184
GFF185 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 197,928 to 198,857
GFF185
GFF186 - hypothetical protein, at 198,898 to 199,665
GFF186
GFF187 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 199,662 to 200,624
GFF187
Position (kb)
197
198
199 Strain fitness (log2 ratio)
-1
0
1
2 at 196.972 kb on - strand at 196.972 kb on - strand at 196.987 kb on + strand at 196.988 kb on - strand at 196.988 kb on - strand at 196.988 kb on - strand at 196.988 kb on - strand at 196.994 kb on + strand at 196.995 kb on - strand at 197.047 kb on + strand at 197.047 kb on + strand at 197.048 kb on - strand at 197.048 kb on - strand at 197.572 kb on + strand, within GFF184 at 197.572 kb on + strand, within GFF184 at 197.573 kb on - strand, within GFF184 at 198.148 kb on - strand, within GFF185 at 198.529 kb on - strand, within GFF185 at 199.180 kb on + strand, within GFF186 at 199.180 kb on + strand, within GFF186 at 199.225 kb on + strand, within GFF186 at 199.225 kb on + strand, within GFF186 at 199.225 kb on + strand, within GFF186 at 199.225 kb on + strand, within GFF186 at 199.226 kb on - strand, within GFF186 at 199.246 kb on + strand, within GFF186 at 199.426 kb on + strand, within GFF186 at 199.687 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 3 remove 196,972 - -0.1 196,972 - +1.4 196,987 + -0.2 196,988 - +0.3 196,988 - -0.4 196,988 - -1.6 196,988 - -1.0 196,994 + +0.1 196,995 - -0.7 197,047 + +1.1 197,047 + +0.3 197,048 - +0.2 197,048 - -0.4 197,572 + GFF184 0.62 +0.1 197,572 + GFF184 0.62 -0.2 197,573 - GFF184 0.62 +2.0 198,148 - GFF185 0.24 +1.2 198,529 - GFF185 0.65 -0.2 199,180 + GFF186 0.37 +0.3 199,180 + GFF186 0.37 +0.3 199,225 + GFF186 0.43 +0.4 199,225 + GFF186 0.43 -0.2 199,225 + GFF186 0.43 +0.5 199,225 + GFF186 0.43 +1.2 199,226 - GFF186 0.43 -0.7 199,246 + GFF186 0.45 -1.1 199,426 + GFF186 0.69 -0.6 199,687 - +0.3
Or see this region's nucleotide sequence