Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF119

Experiment: Ying_OrganicAcid7 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF118 and GFF119 overlap by 1 nucleotidesGFF119 and GFF120 are separated by 102 nucleotides GFF118 - Homolog of E. coli HemX protein, at 129,083 to 130,261 GFF118 GFF119 - Uncharacterized protein EC-HemY, likely associated with heme metabolism based on gene clustering with hemC, hemD in Proteobacteria (unrelated to HemY-type PPO in GramPositives), at 130,261 to 131,565 GFF119 GFF120 - Ribonuclease E (EC 3.1.26.12), at 131,668 to 134,763 GFF120 Position (kb) 130 131 132Strain fitness (log2 ratio) -1 0 1at 129.923 kb on + strand, within GFF118at 130.387 kb on + strandat 130.450 kb on + strand, within GFF119at 130.450 kb on + strand, within GFF119at 130.451 kb on - strand, within GFF119at 130.451 kb on - strand, within GFF119at 131.073 kb on + strand, within GFF119at 131.074 kb on - strand, within GFF119at 131.074 kb on - strand, within GFF119at 131.371 kb on + strand, within GFF119at 131.372 kb on - strand, within GFF119at 131.372 kb on - strand, within GFF119at 131.372 kb on - strand, within GFF119at 132.149 kb on + strand, within GFF120at 132.150 kb on - strand, within GFF120at 132.150 kb on - strand, within GFF120at 132.150 kb on - strand, within GFF120at 132.171 kb on - strand, within GFF120

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 3
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129,923 + GFF118 0.71 -1.2
130,387 + -1.5
130,450 + GFF119 0.14 -1.0
130,450 + GFF119 0.14 -0.2
130,451 - GFF119 0.15 -0.9
130,451 - GFF119 0.15 +0.4
131,073 + GFF119 0.62 -1.3
131,074 - GFF119 0.62 -0.1
131,074 - GFF119 0.62 -1.0
131,371 + GFF119 0.85 -1.2
131,372 - GFF119 0.85 -0.3
131,372 - GFF119 0.85 -1.0
131,372 - GFF119 0.85 -1.2
132,149 + GFF120 0.16 -1.0
132,150 - GFF120 0.16 +1.4
132,150 - GFF120 0.16 -0.1
132,150 - GFF120 0.16 +0.1
132,171 - GFF120 0.16 -0.5

Or see this region's nucleotide sequence