Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF654

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF652 and GFF653 overlap by 4 nucleotidesGFF653 and GFF654 are separated by 11 nucleotidesGFF654 and GFF655 overlap by 4 nucleotides GFF652 - Dihydroorotase (EC 3.5.2.3), at 332,741 to 334,066 GFF652 GFF653 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 334,063 to 334,800 GFF653 GFF654 - Glyoxalase family protein, at 334,812 to 335,165 GFF654 GFF655 - Sigma-54 activated regulatory protein, at 335,162 to 336,391 GFF655 Position (kb) 334 335 336Strain fitness (log2 ratio) -1 0 1 2at 334.399 kb on + strand, within GFF653at 334.711 kb on + strand, within GFF653at 334.798 kb on + strandat 334.846 kb on - strandat 334.846 kb on - strandat 334.846 kb on - strandat 334.846 kb on - strandat 334.846 kb on - strandat 334.890 kb on + strand, within GFF654at 334.890 kb on + strand, within GFF654at 334.891 kb on - strand, within GFF654at 334.891 kb on - strand, within GFF654at 335.019 kb on + strand, within GFF654at 335.020 kb on - strand, within GFF654at 335.202 kb on + strandat 335.203 kb on - strandat 335.754 kb on + strand, within GFF655at 335.754 kb on + strand, within GFF655at 335.754 kb on + strand, within GFF655at 335.754 kb on + strand, within GFF655at 335.754 kb on + strand, within GFF655at 335.755 kb on - strand, within GFF655

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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334,399 + GFF653 0.46 -0.6
334,711 + GFF653 0.88 +0.5
334,798 + -0.4
334,846 - -0.7
334,846 - -0.9
334,846 - -0.1
334,846 - +0.3
334,846 - +0.8
334,890 + GFF654 0.22 +0.8
334,890 + GFF654 0.22 +0.9
334,891 - GFF654 0.22 +0.6
334,891 - GFF654 0.22 -0.9
335,019 + GFF654 0.58 +0.1
335,020 - GFF654 0.59 -1.3
335,202 + +0.9
335,203 - +2.5
335,754 + GFF655 0.48 +0.1
335,754 + GFF655 0.48 -0.5
335,754 + GFF655 0.48 +0.0
335,754 + GFF655 0.48 +0.7
335,754 + GFF655 0.48 -0.9
335,755 - GFF655 0.48 +0.3

Or see this region's nucleotide sequence