Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF5253

Experiment: Ying_OrganicAcid7 rep A; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5252 and GFF5253 are separated by 160 nucleotidesGFF5253 and GFF5254 overlap by 4 nucleotidesGFF5254 and GFF5255 are separated by 19 nucleotides GFF5252 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 2,267,836 to 2,268,762 GFF5252 GFF5253 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 2,268,923 to 2,270,455 GFF5253 GFF5254 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 2,270,452 to 2,271,390 GFF5254 GFF5255 - ABC transporter substrate-binding protein, at 2,271,410 to 2,272,357 GFF5255 Position (kb) 2268 2269 2270 2271Strain fitness (log2 ratio) -2 -1 0 1at 2268.040 kb on + strand, within GFF5252at 2268.041 kb on - strand, within GFF5252at 2268.041 kb on - strand, within GFF5252at 2268.094 kb on + strand, within GFF5252at 2268.094 kb on + strand, within GFF5252at 2268.337 kb on + strand, within GFF5252at 2268.337 kb on + strand, within GFF5252at 2268.532 kb on + strand, within GFF5252at 2268.533 kb on - strand, within GFF5252at 2268.553 kb on + strand, within GFF5252at 2268.656 kb on - strand, within GFF5252at 2268.807 kb on + strandat 2268.808 kb on - strandat 2268.810 kb on + strandat 2268.824 kb on - strandat 2268.824 kb on - strandat 2268.829 kb on + strandat 2268.829 kb on + strandat 2268.830 kb on - strandat 2269.133 kb on + strand, within GFF5253at 2269.134 kb on + strand, within GFF5253at 2269.202 kb on + strand, within GFF5253at 2269.202 kb on + strand, within GFF5253at 2269.203 kb on - strand, within GFF5253at 2269.203 kb on - strand, within GFF5253at 2269.203 kb on - strand, within GFF5253at 2269.203 kb on - strand, within GFF5253at 2269.203 kb on - strand, within GFF5253at 2269.307 kb on + strand, within GFF5253at 2269.307 kb on + strand, within GFF5253at 2269.308 kb on - strand, within GFF5253at 2269.454 kb on + strand, within GFF5253at 2269.455 kb on - strand, within GFF5253at 2269.499 kb on + strand, within GFF5253at 2269.533 kb on - strand, within GFF5253at 2270.157 kb on - strand, within GFF5253at 2270.384 kb on + strandat 2270.827 kb on + strand, within GFF5254at 2270.828 kb on - strand, within GFF5254at 2270.887 kb on + strand, within GFF5254at 2270.887 kb on + strand, within GFF5254at 2271.131 kb on - strand, within GFF5254at 2271.389 kb on + strandat 2271.389 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3
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2,268,040 + GFF5252 0.22 -0.3
2,268,041 - GFF5252 0.22 -0.1
2,268,041 - GFF5252 0.22 +0.9
2,268,094 + GFF5252 0.28 +0.2
2,268,094 + GFF5252 0.28 -0.2
2,268,337 + GFF5252 0.54 -0.3
2,268,337 + GFF5252 0.54 +1.1
2,268,532 + GFF5252 0.75 -0.2
2,268,533 - GFF5252 0.75 -0.3
2,268,553 + GFF5252 0.77 -1.2
2,268,656 - GFF5252 0.88 +1.2
2,268,807 + +0.1
2,268,808 - -0.5
2,268,810 + -0.3
2,268,824 - -0.3
2,268,824 - +0.1
2,268,829 + -0.3
2,268,829 + +0.4
2,268,830 - +0.6
2,269,133 + GFF5253 0.14 -0.9
2,269,134 + GFF5253 0.14 -0.2
2,269,202 + GFF5253 0.18 -0.2
2,269,202 + GFF5253 0.18 -0.1
2,269,203 - GFF5253 0.18 +0.2
2,269,203 - GFF5253 0.18 +0.2
2,269,203 - GFF5253 0.18 -0.9
2,269,203 - GFF5253 0.18 +0.7
2,269,203 - GFF5253 0.18 +0.7
2,269,307 + GFF5253 0.25 +0.3
2,269,307 + GFF5253 0.25 +0.2
2,269,308 - GFF5253 0.25 +0.7
2,269,454 + GFF5253 0.35 -0.6
2,269,455 - GFF5253 0.35 -0.2
2,269,499 + GFF5253 0.38 -0.6
2,269,533 - GFF5253 0.40 -1.6
2,270,157 - GFF5253 0.80 -0.8
2,270,384 + -2.0
2,270,827 + GFF5254 0.40 +0.4
2,270,828 - GFF5254 0.40 +0.7
2,270,887 + GFF5254 0.46 +0.7
2,270,887 + GFF5254 0.46 -0.3
2,271,131 - GFF5254 0.72 -0.3
2,271,389 + +0.1
2,271,389 - +0.4

Or see this region's nucleotide sequence