Experiment: Ying_OrganicAcid7 rep A; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2865 and GFF2866 are separated by 24 nucleotides GFF2866 and GFF2867 overlap by 7 nucleotides GFF2867 and GFF2868 are separated by 137 nucleotides
GFF2865 - Cytosine deaminase (EC 3.5.4.1), at 452,869 to 454,155
GFF2865
GFF2866 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 454,180 to 455,700
GFF2866
GFF2867 - hypothetical protein, at 455,694 to 456,098
GFF2867
GFF2868 - Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-), at 456,236 to 457,279
GFF2868
Position (kb) 454
455
456 Strain fitness (log2 ratio) -1
0
1 at 454.061 kb on + strand at 454.287 kb on - strand at 455.210 kb on + strand, within GFF2866 at 455.210 kb on + strand, within GFF2866 at 455.211 kb on - strand, within GFF2866 at 455.421 kb on - strand, within GFF2866 at 455.421 kb on - strand, within GFF2866 at 455.424 kb on - strand, within GFF2866 at 455.424 kb on - strand, within GFF2866 at 455.505 kb on - strand, within GFF2866 at 455.505 kb on - strand, within GFF2866 at 455.801 kb on - strand, within GFF2867 at 455.801 kb on - strand, within GFF2867 at 456.285 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep A; time point 3 remove 454,061 + -1.0 454,287 - +0.6 455,210 + GFF2866 0.68 -0.7 455,210 + GFF2866 0.68 -1.2 455,211 - GFF2866 0.68 +0.3 455,421 - GFF2866 0.82 +0.3 455,421 - GFF2866 0.82 +0.8 455,424 - GFF2866 0.82 +0.7 455,424 - GFF2866 0.82 +0.1 455,505 - GFF2866 0.87 +0.7 455,505 - GFF2866 0.87 -0.1 455,801 - GFF2867 0.26 +1.0 455,801 - GFF2867 0.26 +0.6 456,285 + +0.6
Or see this region's nucleotide sequence