Experiment: R2A 1 x (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BACOVA_04395 and BACOVA_04396 are separated by 51 nucleotides BACOVA_04396 and BACOVA_04397 overlap by 4 nucleotides BACOVA_04397 and BACOVA_04398 are separated by 115 nucleotides BACOVA_04398 and BACOVA_04400 are separated by 125 nucleotides BACOVA_04400 and BACOVA_04399 overlap by 67 nucleotides
BACOVA_04395: BACOVA_04395 - pullulanase, type I, at 307,513 to 309,519
_04395
BACOVA_04396: BACOVA_04396 - crossover junction endodeoxyribonuclease RuvC, at 309,571 to 310,137
_04396
BACOVA_04397: BACOVA_04397 - hypothetical protein, at 310,134 to 310,436
_04397
BACOVA_04398: BACOVA_04398 - tRNA-Ala, at 310,552 to 310,625
_04398
BACOVA_04400: BACOVA_04400 - hypothetical protein, at 310,751 to 310,888
_04400
BACOVA_04399: BACOVA_04399 - Sigma-70 region 2, at 310,822 to 311,481
_04399
Position (kb)
310
311 Strain fitness (log2 ratio)
-1
0
1
2 at 309.209 kb on + strand, within BACOVA_04395 at 309.277 kb on + strand, within BACOVA_04395 at 309.314 kb on + strand, within BACOVA_04395 at 309.314 kb on + strand, within BACOVA_04395 at 309.315 kb on - strand, within BACOVA_04395 at 309.328 kb on + strand at 309.328 kb on + strand at 309.393 kb on + strand at 309.434 kb on + strand at 309.434 kb on + strand at 309.434 kb on + strand at 309.435 kb on - strand at 309.435 kb on - strand at 309.464 kb on - strand at 309.490 kb on + strand at 309.500 kb on + strand at 309.501 kb on - strand at 309.504 kb on + strand at 309.504 kb on + strand at 309.532 kb on + strand at 309.547 kb on + strand at 309.547 kb on + strand at 309.548 kb on - strand at 309.548 kb on - strand at 309.573 kb on - strand at 309.601 kb on - strand at 309.615 kb on + strand at 309.619 kb on - strand at 309.619 kb on - strand at 309.702 kb on + strand, within BACOVA_04396 at 309.992 kb on + strand, within BACOVA_04396 at 310.141 kb on - strand at 310.141 kb on - strand at 310.176 kb on - strand, within BACOVA_04397 at 310.176 kb on - strand, within BACOVA_04397 at 310.181 kb on + strand, within BACOVA_04397 at 310.213 kb on - strand, within BACOVA_04397 at 310.277 kb on - strand, within BACOVA_04397 at 310.303 kb on - strand, within BACOVA_04397 at 310.306 kb on - strand, within BACOVA_04397 at 310.359 kb on - strand, within BACOVA_04397 at 310.437 kb on - strand at 310.437 kb on - strand at 310.456 kb on - strand at 310.456 kb on - strand at 310.471 kb on - strand at 310.478 kb on - strand at 310.487 kb on + strand at 310.496 kb on - strand at 310.538 kb on + strand at 310.538 kb on + strand at 310.541 kb on - strand at 310.579 kb on + strand, within BACOVA_04398 at 310.595 kb on - strand, within BACOVA_04398 at 310.647 kb on - strand at 310.729 kb on + strand at 310.730 kb on - strand at 310.899 kb on + strand, within BACOVA_04399 at 310.904 kb on + strand, within BACOVA_04399 at 310.937 kb on + strand, within BACOVA_04399 at 310.938 kb on - strand, within BACOVA_04399 at 310.938 kb on - strand, within BACOVA_04399 at 310.943 kb on + strand, within BACOVA_04399 at 310.944 kb on - strand, within BACOVA_04399 at 310.951 kb on - strand, within BACOVA_04399 at 310.991 kb on - strand, within BACOVA_04399 at 310.991 kb on - strand, within BACOVA_04399 at 310.994 kb on - strand, within BACOVA_04399 at 310.994 kb on - strand, within BACOVA_04399 at 310.994 kb on - strand, within BACOVA_04399 at 310.995 kb on + strand, within BACOVA_04399 at 311.004 kb on - strand, within BACOVA_04399 at 311.007 kb on + strand, within BACOVA_04399 at 311.008 kb on - strand, within BACOVA_04399 at 311.008 kb on - strand, within BACOVA_04399 at 311.011 kb on + strand, within BACOVA_04399 at 311.011 kb on + strand, within BACOVA_04399 at 311.026 kb on - strand, within BACOVA_04399 at 311.095 kb on + strand, within BACOVA_04399 at 311.097 kb on + strand, within BACOVA_04399 at 311.098 kb on - strand, within BACOVA_04399 at 311.106 kb on + strand, within BACOVA_04399 at 311.131 kb on + strand, within BACOVA_04399 at 311.131 kb on + strand, within BACOVA_04399 at 311.132 kb on - strand, within BACOVA_04399 at 311.184 kb on - strand, within BACOVA_04399 at 311.219 kb on - strand, within BACOVA_04399 at 311.222 kb on + strand, within BACOVA_04399 at 311.222 kb on + strand, within BACOVA_04399 at 311.222 kb on + strand, within BACOVA_04399 at 311.222 kb on + strand, within BACOVA_04399 at 311.224 kb on + strand, within BACOVA_04399 at 311.224 kb on + strand, within BACOVA_04399 at 311.225 kb on - strand, within BACOVA_04399 at 311.225 kb on - strand, within BACOVA_04399 at 311.225 kb on - strand, within BACOVA_04399 at 311.225 kb on - strand, within BACOVA_04399 at 311.226 kb on + strand, within BACOVA_04399 at 311.229 kb on - strand, within BACOVA_04399 at 311.229 kb on - strand, within BACOVA_04399 at 311.324 kb on - strand, within BACOVA_04399 at 311.324 kb on - strand, within BACOVA_04399 at 311.324 kb on - strand, within BACOVA_04399 at 311.376 kb on + strand, within BACOVA_04399 at 311.389 kb on - strand, within BACOVA_04399 at 311.401 kb on + strand, within BACOVA_04399 at 311.402 kb on - strand, within BACOVA_04399 at 311.402 kb on - strand, within BACOVA_04399 at 311.431 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A 1 x (C) complex media remove 309,209 + BACOVA_04395 0.85 +0.2 309,277 + BACOVA_04395 0.88 -0.7 309,314 + BACOVA_04395 0.90 +0.9 309,314 + BACOVA_04395 0.90 -0.7 309,315 - BACOVA_04395 0.90 -0.3 309,328 + -0.7 309,328 + +0.1 309,393 + +0.1 309,434 + +0.3 309,434 + +0.5 309,434 + +1.3 309,435 - +0.5 309,435 - -1.3 309,464 - -0.7 309,490 + +0.9 309,500 + +0.1 309,501 - -0.9 309,504 + -0.1 309,504 + -0.3 309,532 + -0.6 309,547 + +0.9 309,547 + -0.1 309,548 - -0.1 309,548 - +0.5 309,573 - -0.1 309,601 - +0.6 309,615 + -1.1 309,619 - +0.1 309,619 - -0.1 309,702 + BACOVA_04396 0.23 +0.9 309,992 + BACOVA_04396 0.74 -0.1 310,141 - -0.7 310,141 - -0.4 310,176 - BACOVA_04397 0.14 -0.1 310,176 - BACOVA_04397 0.14 +0.9 310,181 + BACOVA_04397 0.16 +1.9 310,213 - BACOVA_04397 0.26 -1.0 310,277 - BACOVA_04397 0.47 -0.5 310,303 - BACOVA_04397 0.56 -0.7 310,306 - BACOVA_04397 0.57 -0.7 310,359 - BACOVA_04397 0.74 -0.1 310,437 - +1.1 310,437 - +0.4 310,456 - +1.1 310,456 - +0.1 310,471 - +0.8 310,478 - -0.9 310,487 + -1.1 310,496 - +1.5 310,538 + -0.4 310,538 + -0.3 310,541 - +1.7 310,579 + BACOVA_04398 0.36 -0.4 310,595 - BACOVA_04398 0.58 -0.3 310,647 - -1.3 310,729 + +0.1 310,730 - +0.3 310,899 + BACOVA_04399 0.12 +0.3 310,904 + BACOVA_04399 0.12 +0.6 310,937 + BACOVA_04399 0.17 -0.4 310,938 - BACOVA_04399 0.18 -1.2 310,938 - BACOVA_04399 0.18 +1.3 310,943 + BACOVA_04399 0.18 +0.5 310,944 - BACOVA_04399 0.18 +0.8 310,951 - BACOVA_04399 0.20 +0.9 310,991 - BACOVA_04399 0.26 +0.4 310,991 - BACOVA_04399 0.26 +0.9 310,994 - BACOVA_04399 0.26 +0.9 310,994 - BACOVA_04399 0.26 -1.4 310,994 - BACOVA_04399 0.26 +0.3 310,995 + BACOVA_04399 0.26 +0.2 311,004 - BACOVA_04399 0.28 -0.0 311,007 + BACOVA_04399 0.28 -0.3 311,008 - BACOVA_04399 0.28 -1.0 311,008 - BACOVA_04399 0.28 +0.2 311,011 + BACOVA_04399 0.29 -0.1 311,011 + BACOVA_04399 0.29 +0.9 311,026 - BACOVA_04399 0.31 -0.2 311,095 + BACOVA_04399 0.41 -1.3 311,097 + BACOVA_04399 0.42 -1.3 311,098 - BACOVA_04399 0.42 +0.6 311,106 + BACOVA_04399 0.43 -1.1 311,131 + BACOVA_04399 0.47 +0.5 311,131 + BACOVA_04399 0.47 -0.6 311,132 - BACOVA_04399 0.47 +0.2 311,184 - BACOVA_04399 0.55 +0.0 311,219 - BACOVA_04399 0.60 -1.7 311,222 + BACOVA_04399 0.61 +0.2 311,222 + BACOVA_04399 0.61 +0.6 311,222 + BACOVA_04399 0.61 +0.3 311,222 + BACOVA_04399 0.61 +1.6 311,224 + BACOVA_04399 0.61 -0.5 311,224 + BACOVA_04399 0.61 -1.0 311,225 - BACOVA_04399 0.61 +0.5 311,225 - BACOVA_04399 0.61 +0.3 311,225 - BACOVA_04399 0.61 -0.1 311,225 - BACOVA_04399 0.61 +1.2 311,226 + BACOVA_04399 0.61 +1.1 311,229 - BACOVA_04399 0.62 -0.2 311,229 - BACOVA_04399 0.62 +0.6 311,324 - BACOVA_04399 0.76 +0.4 311,324 - BACOVA_04399 0.76 +0.4 311,324 - BACOVA_04399 0.76 +0.2 311,376 + BACOVA_04399 0.84 -0.2 311,389 - BACOVA_04399 0.86 +1.9 311,401 + BACOVA_04399 0.88 +0.9 311,402 - BACOVA_04399 0.88 -0.1 311,402 - BACOVA_04399 0.88 -0.8 311,431 - +0.4
Or see this region's nucleotide sequence