Experiment: HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS04720 and A4249_RS04725 are separated by 4 nucleotides A4249_RS04725 and A4249_RS04730 are separated by 10 nucleotides
A4249_RS04720: A4249_RS04720 - acyl-CoA dehydrogenase family protein, at 981,179 to 982,360
_RS04720
A4249_RS04725: A4249_RS04725 - acyl-CoA dehydrogenase, at 982,365 to 983,483
_RS04725
A4249_RS04730: A4249_RS04730 - Hsp70 family protein, at 983,494 to 984,813
_RS04730
Position (kb)
982
983
984 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 981.377 kb on + strand, within A4249_RS04720 at 981.377 kb on + strand, within A4249_RS04720 at 981.378 kb on - strand, within A4249_RS04720 at 981.572 kb on + strand, within A4249_RS04720 at 981.573 kb on - strand, within A4249_RS04720 at 981.573 kb on - strand, within A4249_RS04720 at 981.674 kb on + strand, within A4249_RS04720 at 981.674 kb on + strand, within A4249_RS04720 at 981.675 kb on - strand, within A4249_RS04720 at 981.834 kb on - strand, within A4249_RS04720 at 981.905 kb on + strand, within A4249_RS04720 at 981.905 kb on + strand, within A4249_RS04720 at 981.905 kb on + strand, within A4249_RS04720 at 981.905 kb on + strand, within A4249_RS04720 at 981.906 kb on - strand, within A4249_RS04720 at 981.906 kb on - strand, within A4249_RS04720 at 981.906 kb on - strand, within A4249_RS04720 at 981.906 kb on - strand, within A4249_RS04720 at 981.906 kb on - strand, within A4249_RS04720 at 981.906 kb on - strand, within A4249_RS04720 at 982.151 kb on + strand, within A4249_RS04720 at 982.151 kb on + strand, within A4249_RS04720 at 982.151 kb on + strand, within A4249_RS04720 at 982.151 kb on + strand, within A4249_RS04720 at 982.151 kb on + strand, within A4249_RS04720 at 982.151 kb on + strand, within A4249_RS04720 at 982.151 kb on + strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.152 kb on - strand, within A4249_RS04720 at 982.154 kb on + strand, within A4249_RS04720 at 982.154 kb on + strand, within A4249_RS04720 at 982.154 kb on + strand, within A4249_RS04720 at 982.155 kb on - strand, within A4249_RS04720 at 982.155 kb on - strand, within A4249_RS04720 at 982.155 kb on - strand, within A4249_RS04720 at 982.155 kb on - strand, within A4249_RS04720 at 982.289 kb on - strand at 982.317 kb on + strand at 982.317 kb on + strand at 982.317 kb on + strand at 982.318 kb on - strand at 982.318 kb on - strand at 982.318 kb on - strand at 982.318 kb on - strand at 982.318 kb on - strand at 982.630 kb on - strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.897 kb on + strand, within A4249_RS04725 at 982.898 kb on - strand, within A4249_RS04725 at 982.898 kb on - strand, within A4249_RS04725 at 982.898 kb on - strand, within A4249_RS04725 at 982.898 kb on - strand, within A4249_RS04725 at 982.898 kb on - strand, within A4249_RS04725 at 982.961 kb on + strand, within A4249_RS04725 at 982.961 kb on + strand, within A4249_RS04725 at 982.961 kb on + strand, within A4249_RS04725 at 982.962 kb on - strand, within A4249_RS04725 at 982.962 kb on - strand, within A4249_RS04725 at 983.130 kb on + strand, within A4249_RS04725 at 983.130 kb on + strand, within A4249_RS04725 at 983.131 kb on - strand, within A4249_RS04725 at 983.131 kb on - strand, within A4249_RS04725 at 983.481 kb on + strand at 983.481 kb on + strand at 983.481 kb on + strand at 983.482 kb on - strand at 983.482 kb on - strand at 983.482 kb on - strand at 983.496 kb on - strand at 983.496 kb on - strand at 983.867 kb on + strand, within A4249_RS04730 at 983.867 kb on + strand, within A4249_RS04730 at 984.017 kb on + strand, within A4249_RS04730 at 984.017 kb on + strand, within A4249_RS04730 at 984.017 kb on + strand, within A4249_RS04730 at 984.017 kb on + strand, within A4249_RS04730 at 984.017 kb on + strand, within A4249_RS04730 at 984.018 kb on - strand, within A4249_RS04730 at 984.018 kb on - strand, within A4249_RS04730 at 984.018 kb on - strand, within A4249_RS04730 at 984.018 kb on - strand, within A4249_RS04730 at 984.018 kb on - strand, within A4249_RS04730 at 984.018 kb on - strand, within A4249_RS04730 at 984.020 kb on + strand, within A4249_RS04730 at 984.033 kb on + strand, within A4249_RS04730 at 984.059 kb on + strand, within A4249_RS04730 at 984.059 kb on + strand, within A4249_RS04730 at 984.059 kb on + strand, within A4249_RS04730 at 984.059 kb on + strand, within A4249_RS04730 at 984.060 kb on - strand, within A4249_RS04730 at 984.060 kb on - strand, within A4249_RS04730 at 984.060 kb on - strand, within A4249_RS04730 at 984.060 kb on - strand, within A4249_RS04730 at 984.060 kb on - strand, within A4249_RS04730 at 984.180 kb on + strand, within A4249_RS04730 at 984.180 kb on + strand, within A4249_RS04730 at 984.180 kb on + strand, within A4249_RS04730 at 984.180 kb on + strand, within A4249_RS04730 at 984.181 kb on - strand, within A4249_RS04730 at 984.181 kb on - strand, within A4249_RS04730 at 984.181 kb on - strand, within A4249_RS04730 at 984.181 kb on - strand, within A4249_RS04730 at 984.181 kb on - strand, within A4249_RS04730 at 984.181 kb on - strand, within A4249_RS04730 at 984.181 kb on - strand, within A4249_RS04730 at 984.255 kb on + strand, within A4249_RS04730 at 984.256 kb on - strand, within A4249_RS04730 at 984.256 kb on - strand, within A4249_RS04730 at 984.272 kb on + strand, within A4249_RS04730 at 984.272 kb on + strand, within A4249_RS04730 at 984.272 kb on + strand, within A4249_RS04730 at 984.272 kb on + strand, within A4249_RS04730 at 984.272 kb on + strand, within A4249_RS04730 at 984.272 kb on + strand, within A4249_RS04730 at 984.272 kb on + strand, within A4249_RS04730 at 984.273 kb on - strand, within A4249_RS04730 at 984.273 kb on - strand, within A4249_RS04730 at 984.303 kb on - strand, within A4249_RS04730 at 984.303 kb on - strand, within A4249_RS04730
Per-strain Table
Position Strand Gene LocusTag Fraction HMW_DNA_from_Pseudomonas_EB271-B4-1 46.9 ppm (C) remove 981,377 + A4249_RS04720 0.17 +0.3 981,377 + A4249_RS04720 0.17 +0.1 981,378 - A4249_RS04720 0.17 -0.3 981,572 + A4249_RS04720 0.33 -0.4 981,573 - A4249_RS04720 0.33 -1.1 981,573 - A4249_RS04720 0.33 +0.3 981,674 + A4249_RS04720 0.42 -0.9 981,674 + A4249_RS04720 0.42 +0.1 981,675 - A4249_RS04720 0.42 -0.5 981,834 - A4249_RS04720 0.55 -0.4 981,905 + A4249_RS04720 0.61 +0.5 981,905 + A4249_RS04720 0.61 -1.2 981,905 + A4249_RS04720 0.61 +1.5 981,905 + A4249_RS04720 0.61 +0.7 981,906 - A4249_RS04720 0.62 -0.6 981,906 - A4249_RS04720 0.62 +0.0 981,906 - A4249_RS04720 0.62 -0.9 981,906 - A4249_RS04720 0.62 -0.7 981,906 - A4249_RS04720 0.62 +0.8 981,906 - A4249_RS04720 0.62 +0.4 982,151 + A4249_RS04720 0.82 +0.6 982,151 + A4249_RS04720 0.82 -0.0 982,151 + A4249_RS04720 0.82 -0.3 982,151 + A4249_RS04720 0.82 -0.1 982,151 + A4249_RS04720 0.82 -0.1 982,151 + A4249_RS04720 0.82 -1.2 982,151 + A4249_RS04720 0.82 -0.3 982,152 - A4249_RS04720 0.82 +0.2 982,152 - A4249_RS04720 0.82 +0.7 982,152 - A4249_RS04720 0.82 -0.5 982,152 - A4249_RS04720 0.82 -2.8 982,152 - A4249_RS04720 0.82 -0.1 982,152 - A4249_RS04720 0.82 +1.1 982,152 - A4249_RS04720 0.82 -0.7 982,152 - A4249_RS04720 0.82 -0.7 982,152 - A4249_RS04720 0.82 +0.2 982,154 + A4249_RS04720 0.82 +1.1 982,154 + A4249_RS04720 0.82 +1.1 982,154 + A4249_RS04720 0.82 -0.1 982,155 - A4249_RS04720 0.83 -0.8 982,155 - A4249_RS04720 0.83 -1.6 982,155 - A4249_RS04720 0.83 +0.7 982,155 - A4249_RS04720 0.83 +0.9 982,289 - -1.9 982,317 + +0.5 982,317 + +0.3 982,317 + +0.3 982,318 - -0.0 982,318 - +0.4 982,318 - +0.0 982,318 - +0.8 982,318 - +0.8 982,630 - A4249_RS04725 0.24 -0.1 982,897 + A4249_RS04725 0.48 -0.2 982,897 + A4249_RS04725 0.48 -0.2 982,897 + A4249_RS04725 0.48 -0.4 982,897 + A4249_RS04725 0.48 -0.3 982,897 + A4249_RS04725 0.48 +0.1 982,897 + A4249_RS04725 0.48 +0.7 982,897 + A4249_RS04725 0.48 +0.0 982,897 + A4249_RS04725 0.48 -0.3 982,897 + A4249_RS04725 0.48 +1.0 982,898 - A4249_RS04725 0.48 -0.4 982,898 - A4249_RS04725 0.48 -0.4 982,898 - A4249_RS04725 0.48 -0.3 982,898 - A4249_RS04725 0.48 +0.6 982,898 - A4249_RS04725 0.48 +1.3 982,961 + A4249_RS04725 0.53 -0.5 982,961 + A4249_RS04725 0.53 -0.8 982,961 + A4249_RS04725 0.53 +0.6 982,962 - A4249_RS04725 0.53 -0.3 982,962 - A4249_RS04725 0.53 +0.4 983,130 + A4249_RS04725 0.68 +1.1 983,130 + A4249_RS04725 0.68 +0.1 983,131 - A4249_RS04725 0.68 +0.1 983,131 - A4249_RS04725 0.68 +0.0 983,481 + +0.1 983,481 + -0.4 983,481 + +0.1 983,482 - -0.5 983,482 - -0.9 983,482 - +0.7 983,496 - -0.2 983,496 - -0.4 983,867 + A4249_RS04730 0.28 -0.0 983,867 + A4249_RS04730 0.28 -0.6 984,017 + A4249_RS04730 0.40 +0.2 984,017 + A4249_RS04730 0.40 -1.4 984,017 + A4249_RS04730 0.40 +0.4 984,017 + A4249_RS04730 0.40 -1.3 984,017 + A4249_RS04730 0.40 -0.3 984,018 - A4249_RS04730 0.40 -0.5 984,018 - A4249_RS04730 0.40 +0.2 984,018 - A4249_RS04730 0.40 +0.4 984,018 - A4249_RS04730 0.40 +0.3 984,018 - A4249_RS04730 0.40 +0.2 984,018 - A4249_RS04730 0.40 -1.0 984,020 + A4249_RS04730 0.40 +0.4 984,033 + A4249_RS04730 0.41 +0.5 984,059 + A4249_RS04730 0.43 -0.2 984,059 + A4249_RS04730 0.43 -0.7 984,059 + A4249_RS04730 0.43 -0.5 984,059 + A4249_RS04730 0.43 -0.4 984,060 - A4249_RS04730 0.43 +0.9 984,060 - A4249_RS04730 0.43 +1.2 984,060 - A4249_RS04730 0.43 +0.3 984,060 - A4249_RS04730 0.43 +2.3 984,060 - A4249_RS04730 0.43 +1.7 984,180 + A4249_RS04730 0.52 -1.1 984,180 + A4249_RS04730 0.52 -0.5 984,180 + A4249_RS04730 0.52 -0.3 984,180 + A4249_RS04730 0.52 +0.7 984,181 - A4249_RS04730 0.52 +0.3 984,181 - A4249_RS04730 0.52 -0.1 984,181 - A4249_RS04730 0.52 -0.4 984,181 - A4249_RS04730 0.52 +0.4 984,181 - A4249_RS04730 0.52 -1.3 984,181 - A4249_RS04730 0.52 +0.5 984,181 - A4249_RS04730 0.52 +0.3 984,255 + A4249_RS04730 0.58 +0.6 984,256 - A4249_RS04730 0.58 +0.4 984,256 - A4249_RS04730 0.58 -1.3 984,272 + A4249_RS04730 0.59 -0.4 984,272 + A4249_RS04730 0.59 -0.7 984,272 + A4249_RS04730 0.59 -0.3 984,272 + A4249_RS04730 0.59 -0.8 984,272 + A4249_RS04730 0.59 +2.1 984,272 + A4249_RS04730 0.59 -1.1 984,272 + A4249_RS04730 0.59 -0.1 984,273 - A4249_RS04730 0.59 +0.7 984,273 - A4249_RS04730 0.59 -0.1 984,303 - A4249_RS04730 0.61 +0.3 984,303 - A4249_RS04730 0.61 -1.4
Or see this region's nucleotide sequence